Oxidation of Glucose

Question 1

Glycolysis information

Answer 1

• cytoplasm (10 steps)

- 2 ATP go in and 4 come out by substrate-level phosphorylation

Question 2

Glycolysis steps

Answer 2

1. glucose -1 ATP
2. G-6-P
3. F-6-P -1 ATP
4. F1, 6-BP
5. DHAP and Glyceraldehyde-3-P +1 NADH
6. 1,3 BPG +1 ATP
7. 3PG
8. 2PG
9. PEP +1 ATP
10. Pyruvate

Question 3

Pyruvate oxidation information

Answer 3

• occurs at inner membrane of mitochondria

- produces 2 NADH

Question 4

Pyruvate oxidation steps

Answer 4

1. 3C pyruvate
   - CO2 leaves (exergonic)
   - +1 NADH (endergonic)
   - CoA is added
2. 2C Acetyl-CoA

Question 5

oxidation of glucose

Answer 5

-biochemical pathway

Question 6

Krebs cycle information

Answer 6

• occurs in matrix of mitochondria (enzyme)
• produces 3 NADH, 1 FADH2, 1 GTP (x2)
• 9 steps, cycles twice

Question 7

Krebs cycle steps

Answer 7

1. 2C Acetyl CoA and 4C OAA -CoA
2. 6C citric acid
3. 6C isocitrate -CO2 +NADH
4. 5C alpha KG -CO2 +NADH +CoA
5. 4C succinyl CoA +GTP/ATP -CoA
6. 4C succinate +FADH2
7. 4C fumerate add H2O
8. 4C malate +NADH
9. 4C OAA

Question 8

Products of oxidation of glucose

Answer 8

glycolysis: 2 ATP and 2 NADH
pyruvate oxidation: 2 NADH
krebs: 6 NADH, 2 FADH2, 2 GTP
ETC: 34 ATP 
-40 total ATP, loses 2 in glycolysis and 2 to nature

Question 9

Electron transport chain information

Answer 9

• occurs in inner mitochondria membrane (embedded)
• as electrons are transferred, energy is lost
• energy is used to pump protons across the membrane from matrix to intermembrane space
• proton gradient is established (used to drive atp synthase)

Question 10

Electron transport chain steps

Answer 10

1. NADH dehydrogenase
2. succinate dehydrogenase
3. BC1 complex
4. cytochrome oxidase complex