Other Important Stuff Flashcards

1
Q

Define denaturation

A

Conversion of double-stranded DNA to single-stranded DNA

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2
Q

Define annealing

A

Conversion of single-stranded DNA to double-stranded DNA

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3
Q

What is the concentration of K+ in cytosol?

A

140 mM

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4
Q

What is the concentration of Na+ in cytosol?

A

12 mM

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5
Q

What is the concentration of K+ in extracellular fluid?

A

4 mM

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6
Q

What is the concentration of Na+ in extracellular fluid?

A

145 mM

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7
Q

What does the Na+K+ATPase transport?

A

3 Na+ outside and 2 K+ into the cell

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8
Q

Describe acetic acid

A

2 carbons

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9
Q

Describe capric acid

A

10 carbons

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10
Q

Describe lauric acid

A

12 carbons

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11
Q

Describe palmitic acid

A

16 carbons

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12
Q

Describe stearic acid

A

18 carbons

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13
Q

Describe palmitoleic acid

A

16 carbons with a double bond at C9

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14
Q

Describe oleic acid

A

18 carbons with a double bond at C9

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15
Q

Describe linoleic acid

A

18 carbons with double bonds at C9 and C12

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16
Q

Describe gamma-linoleic acid

A

18 carbons with double bonds at C6, C9, and C12

17
Q

Describe alpha-linoleic acid

A

18 carbons with double bonds at C9, C12, and C15

18
Q

What can inhibit the proton pump?

A

Cyanide (CN-)

19
Q

What does adding cyanide to E. coli cause?

A

Proton pump will be inhibited, so lactose transport will stop

20
Q

What is the sodium-potassium pump used for?

A

Electrical signalling in neurons

21
Q

What type of system is the chloride-bicarbonate exchanger?

A

Obligatory co-transport

22
Q

What type of process occurs in the chloride-bicarbonate exchanger?

A

Antiport

23
Q

What is the difference between a nucleotide and nucleoside backbone?

A

Nucleotide has a phosphate group attached to the CH2, while nucleoside does not

24
Q

What does it mean when a nucleobase has “ylate” at the end of its name?

A

It has a nucleotide backbone attached to it

25
Q

What does it mean when a nucleobase has “sine” at the end of its name?

A

It has a nucleoside backbone attached to it

26
Q

What is the difference between a nucleotide base and deoxynucleotide base?

A

Deoxynucleotide has an H instead of an OH on the bottom right carbon

27
Q

What types of backbones can adenine be attached to?

A
  • Nucleotide
  • Nucleoside
  • Deoxynucleotide
  • Deoxynucleoside
28
Q

What types of backbones can guanine be attached to?

A
  • Nucleotide
  • Nucleoside
  • Deoxynucleotide
  • Deoxynucleoside
29
Q

What types of backbones can cytosine be attached to?

A
  • Nucleotide
  • Nucleoside
  • Deoxynucleotide
  • Deoxynucleoside
30
Q

What types of backbones can thymine be attached to?

A
  • Deoxynucleotide

- Deoxynucleoside

31
Q

What types of backbones can uracil be attached to?

A
  • Nucleotide

- Nucleoside

32
Q

What does trypsin cleave after?

A

Lysine and arginine

33
Q

What does chymotrpysin cleave after?

A

Phenylalanine, tyrosine, or tryptophan

34
Q

What does cyanogen bromide cleave after?

A

Methionine