Citric Acid Cycle Flashcards
Reactant in step 1
Oxaloacetate
Product in step 1
Citrate
Reactant in step 2
Citrate
Product in step 2
Isocitrate
Reactant in step 3
Isocitrate
Product in step 3
alpha-ketoglutarate
Reactant in step 4
alpha-ketoglutarate
Product in step 4
Succinyl-CoA
Reactant in step 5
Succiny-CoA
Product in step 5
Succinate
Reactant in step 6
Succinate
Product in step 6
Fumarate
Reactant in step 7
Fumarate
Product in step 7
L-malate
Reactant in step 8
L-malate
Product in step 8
Oxaloacetate
Enzyme in step 1
Citrate synthase
Enzyme in step 2
Aconitase
Enzyme in step 3
Isocitrate dehydrogenase
Enzyme in step 4
alpha-ketoglutarate dehydrogenase complex and TPP, lipoate, FAD
Enzyme in step 5
Succinyl-CoA synthetase
Enzyme in step 6
Succinate dehydrogenase
Enzyme in step 7
Fumarase
Enzyme in step 8
Malate dehydrogenase
Coenzyme in step 4
- CoA-SH in, CO2 out
- NAD+ -> NADH
Coenzyme in step 5
- GDP + P -> GTP
- CoA-SH released
Coenzyme in step 6
FAD -> FADH2
Coenzyme in step 7
H2O in
Coenzyme in step 8
NAD+ -> NADH and H+
Is step 1 exergonic or endergonic?
Exergonic
What type of reaction is step 2?
Isomerization
What type of reaction is step 3?
Oxidative decarboxylation
What type of reaction is step 4?
Oxidative decarboxylation
What inhibits the alpha-ketoglutarate dehydrogenase complex?
NADH and succinyl-CoA
What type of reaction is step 5?
Substrate level phosphorylation
Are GTP and ATP energetically different or equivalent?
Equivalent
Which enzyme in the CAC is membrane bound in the mitochondria?
Succinate dehydrogenase (enzyme of step 6)
Is step 8 exergonic or endergonic?
Endergonic
Coenzyme in step 1
CH3-C=O-SCoA + H2O -> HSCoA + H+
Coenzyme in step 2
H2O released and H2O in
Coenzyme in step 3
- H+ and NAD+ -> NADH and H+
- CO2 released
