OTHER BLOOD GROUP SYSTEMS Flashcards
M and N antigens are sensitive to these forms of red cell treatment except:
A. a-chymotrypsin
B. Papain
C. Ficin
D. Trypsin
E. None of the above
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A. a-chymotrypsin
M and N are partially reduced by this
The U antigen is sensitive to which form of enzyme treatment?
A. a-chymotrypsin
B. Papain
C. Ficin
D. Trypsin
E. None of the above
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E. None of the above
U antigen is resistant to all of thes
Which of the ff red cell treatment is the Lutheran antigens sensitive to?
A. Papain
B. DTT
C. Trypsin
D. Ficin
E. None of the above
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C. Trypsin
Sensitive (destrroyed) with trypsin and a-chymotrypsin
Resistant to no effect : papain, ficin, AET, DTT
Which of the ff Kell phenotypes is most confined in African ancestry? A. Js (a+b-) B. Js (a+b+) C. Kp (a-b+) D. Kp (a+b+)
B. Js (a+b+)
19% of blacks are Js (a+b+), 1% are Js (a+b-)
Js (a+) is confined to people of African ancestry
Kell is RESISTANT to which red cell treatment?
A. EDTA-glycine
B. trypsin
C. AET
D. DTT
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B. Trypsin
Kell is sensitive / destroyed by the rest
True / false: suppression of erythropoeisis is associated in anti-K HDFN since Kell glycoproteins appear much earlier in fetal development compared to Rh antigen
True
Which of the ff enzymes is the Duffy antigens RESISTANT to?
A. Trypsin
B. Bromelin
C. Ficin
D. a-chymotrypsin
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A. Trypsin
True / false: in people of African ethnicity, the phenotype Fy (a-b-) is most common; The Duffy antigens, also found on many cells of the body, is also lacking in individuals with this phenotype, thus, the chances of making Anti-Fy(b), -Fy3, and -Fy5 are very high.
False
The Fy(a-b-) phenotype in African ancestry is caused by the silencing of the homozygous FY*02N.01 allele (GATA-1 mutation?) = prevents Fy(b) expression in erythroid (blood cell) tissues. So in this Fy(a-b-) pheno, Fy(b) is lacking only in the red cells, but present in many cells throughout the body so they very rarely make the antibodies.
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Which blood group antigens can be found on the glycoprotein atypical chemokine receptor 1 (ACKR1) of the red cell surface?
A. Kell
B. MNS
C. Kidd
D. Duffy
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D. Duffy
Which of the Duffy antigens is absent in not only in Fy(a-b-) phenotype but also in Rh null phenotype?
A. Fy3
B. Fy5
C. Fy6
D. Fyb
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B. Fy5
Which of these red cell antigens or proteins is used as a receptor for merozoites like P. vivax (malaria)?
A. Band 3
B. HUT ( urea transporter)l
D. ACKR1 (atypical chemokine receptor)
D. ERMAP (erythroblast membrane associated protein)
C. ACKR1
This glycoprotein is present in many organs; but function is unknown
Fy(a-b-) are resistant to malaria; they are NOT necessarily lacking the ACKR1
FY*02N.01 allele mutation may give selective advantage to endemic areas
Band 3 - Diego system
SLC14A1 - kidd system
Which of these red cell antigens or proteins are the Diego antigens found? A. Band 3 B. SLC14A1 D. ACKR1 D. ERMAP
A. Band 3
Has functions in CO2 transport and attachment of RBC membrane to cytoskeleton
Which of the ff is associated with he Yt antigens? A. Fucosylase B. Urea transporter C. Acetylcholinesterase D. Aquaphorins
C. Acetylcholinesterase
Yt antigens can be found here
Glycophorin A (GPA), where M and N antigens are found, is associated with the expression of which antigen?
A. Yt(a)
B. Jk(b)
C. Knopps
D. Wr(b)
D. Wr(b)
Lack of GPA in RBC membrane recults in failure to express Wr(b)
Which of the ff Diego antibodies reflect dosage?
A. Anti-Wr(b)
B. Anti - Yt(b)
C. Anti - ELO
D. Anti - Di(b)
D. Anti - Di(b)
Which of the ff Diego antibodies has shown to have specificity in patients with AIHA?
A. Anti-Wr(b)
B. Anti - Yt(b)
C. Anti - ELO
D. Anti - Di(b)
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A. Anti-Wr(b)
Which of the ff antibodies is produced by the Dombrock null phenotype?
A. Anti -Hy
B. Anti - Gy(a)
C. Anti - Do(b)
D. Anti - Jo(a)
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B. Anti - Gy(a)
Which antigens are found on the red cell´s water transporter, aquaporin-1
A. Do
B. Co
C. Yt
D. Sc
B. Co
Colton antigens
True / false - Fya expression in infants usually reach adult expression at 12 weeks after birth
True
Which ethnicity is Fya antigen most likely expressed in?
A. Caucasian
B. Blacks
C. Asian
D. Hispanic
C. Asian
True / false - Anti-Fyb is less immunogenic than anti - Fya
True
The Jk null phenotype, Jk(a-b-) can potentially produce which antibody?
Anti-Jk3
Which ethnicity is the phenotype Jk(a-b-) most commonly found?
A. Hispanics
B. Hmong (asians)
C. Polynesians
D. Siberians
C. Polynesians
Which red cell protein or antigens are the Kidd antigens located at?
A. Band 3
B. SLC14A1
C. ACKR1
D. ERMAP
B. SLC14A1
Red cell urea transporter
Which method is used to effectively screen donors for the Jk(a-b-) phenotype?
A. Cell lysis with 2M urea
B. Test for agglutination with Rh null red cells
C. RBC inhibition activity with hydatid cyst fluid
D. Donath-Landsteiner test
A. Cell lysis with 2M urea
Normal RBCs are lysed by 2M urea (becomes hypertonic then bursts). Jk(a-b-) doesn´t have the SLC14A1 urea transporter so cells do not hemolyze with 2M urea
RBC inhibition activity with hydatid cyst fluid - antisera for P1 inhibition
Donath-Landsteiner test - test for auto anti-P
How do you differentiate Anti-LW from anti-D?
A. Test plasma with ficin treated cells; anti-LW will react, anti-D will not
B. Test plasma with trypsin treated cells; anti-LW is non reactive, anti-D will react
C. Test plasma with pronase treated cells; anti-LW will react, anti-D will not
D. Test plasma with DTT treated cells; anti-LW is non reactive, anti-D will react
D. Test plasma with DTT treated cells; anti-LW is non reactive, anti-D will react
Anti-LW non reactive and Anti-D is reactive with DTT and pronase treated cells
(Remember anti-D is reactive with DTT)
S and s antigens are sensitive to these forms of red cell treatment except: A. a-chymotrypsin B. Bromelin C. Pronase D. Trypsin E. None of the above
D. Trypsin
Which of the ff high prevalence antigens serves as a receptor for Haemophilus influenza on red cells?
A. Vel
B. ABTI
C. AnWj
D. MAM
C. AnWj
Anti-Tja can be made by which individuals?
A. P+ individuals
B. P- individuals
C. P null individuals
D. Pk individuals
C. P null individuals
Anti- Tja is now renamed Anti-PP1Pk
Which of the ff does NOT apply to PP1Pk antibodies?
A. Can cause severe HTR and HDFN
B. Can efficiently bind complement
C. Are made by p individuals
D. All of the above
E. None of the above
E. None of the above
Choices A-C applies to PP1Pk
Which test can demonstrate the reactivity of autoanti-P?
A. Donath-Landsteiner
B. Neutralization with hydatid cyst fluid
C. Neutralization with saliva
D. Kleihauer-Betke
A. Donath-Landsteiner
Demonstrates the biphasic hemolysin characteristic of autoanti-P
A rare gene deletion at the GPYA locus produces which phenotype?
M-, N-, En(a-)
No GPA is produced = so no M and N antigens produced
An immunized individual with the Ko phenotype ( Kell null) can make which antibody?
Ku
single specificity; the antibody recognizes the universal (Ku) Kell antigen
The M^k M^k phenotype is the null phenotype of which blood group system?
MNS system
this phenotype is produced by the rare silent Mk gene
RBCs type as M-N-S-s-U-En(a-), Wr(a-,b-)
Individuals who have the McLeod phenotype typically also show which RBC deformity?
Acanthocytes
Fy^x is the weak form of which Duffy antigen?
Fyb
there is no anti-Fy^x
Which associated phenoty?pe differentiates Fy5 from Fy3
Fy5 is Rh null, Fy(a-,b-)
Fy5 appears to be a result of the interaction between Rh complexes and Duffy glycoproteins
