Organisation RQP- effect of pH on rate of reaction of amylase

Question 1

step 1

Answer 1

put a drop of iodine solution into every well of a spotting tile

Question 2

step 2

Answer 2

place bunsen burner on heatproof mat, add a tripod and gauze over the bunsen burner and heat a beaker of water on top of the tripod until it is 35°C (measure with thermometer)

Question 3

step 3

Answer 3

use a syringe to add 1cm3 of amylase solution and 1cm3 of pH 5 buffer solution to a boiling tube and put in the beaker with a test tube holder for 5mins

Question 4

step 4

Answer 4

use another syringe to add 5cm3 of starch solution to the boiling tube

Question 5

step 5

Answer 5

immediately mix the contents of boiling tube and start a stopwatch

Question 6

step 6

Answer 6

record how long it takes for the amylase to break down all of the starch by using a pipette to take a fresh sample from the tube every 30secs and put a drop into a well- when iodine soltion stays browny-orange, starch is no longer present

Question 7

step 7

Answer 7

repeat whole experiment with different pH buffer solutions, controlling things such as concentration and volume of amylase solution to make it a fair test

Question 8

rate of reaction formula

Answer 8

• 1000/ time(s)
• rate measures in cm3/s