Cells RQP- effect of antiseptics or antibiotics on bacterial growth

Question 1

step 1

Answer 1

soak filter paper discs in different types or concentrations of antibiotics

Question 2

step 2

Answer 2

soak a disc in sterile water instead of antibiotic as a control to be sure that the difference in growth of bacteria is due to the antibiotic alone

Question 3

step 3

Answer 3

place the discs on an agar plate that has an even covering of bacteria, leaving space between the discs

Question 4

step 4

Answer 4

leave the plates for 48 hours at 25°C

Question 5

step 5

Answer 5

measure the clear area around the soaked filter paper discs (inhibtion area)
the larger the inihbtion zone, the more effective the antibiotic is against the bacteria