Organelle Biogenesis Flashcards
Chaperone protein
protein that assists with the folding or unfolding and assembly and disassembly of other macromolecular structures
Importins
a type of karyopherin that transports protein molecules into the nucleus by binding to specific recognition sequences, called nuclear localization signal (NLS)
karyopherin
Karyopherins are a group of proteins involved in transporting molecules between the cytoplasm and the nucleus of a eukaryotic cell
Nucleoporins
a family of proteins which are the constituent building blocks of the nuclear pore complex (NPC)
What drives directional transport through the nuclear pore?
Ran GTPase
Ran GTPase
Ran is a small 25 kDa protein that is involved in transport into and out of the cell nucleus during interphase and also involved in mitosis
Exportins
a type of karyopherin that transports protein molecules out of the nucleus by binding to specific recognition sequences, called nuclear export signal (NES)
Ran GAP
Ran GTPase activating protein, converts Ran GTP to Ran GDP
Ran GEF
Ran Guanine Exchange factor, converts Ran GDP to Ran GTP
Signal peptidase (protease)
Remove N-terminal of proteins imported into the Mitochondria
How are proteins synthesized in the cytoplasm directed toward the Mitochondria?
Targeting sequence found at the N-terminal of the protein
What are the 4 three protein translocators?
Tom complex, Tim 23 complex, Tim 22 complex, Oxa complex
SAM
sorting and assembly machinery
What does organelle Targeting require?
chaperonins and ATP
Contact sites
site of protein movement through both membranes of the mitochondria
