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PER 1001 > Oral Microbiome > Flashcards

Oral Microbiome Flashcards

1
Q

Both organims benefit

A

Symbiotic

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2
Q

Organism exsit in the presence of another but without affecting them but can become pathogenic if certian conditions arise

A

Commensals

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3
Q

Commensals in certian niches but can become pathogenic witha high virulance factor.

A

Opportunistic

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4
Q

an ecological community of symbiotic, commensal and pathogenic microorganisms.

A

Oral microbiome

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5
Q

If microorganims become imbalanaced what happnes?

A

Oral health suffers

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6
Q

What characterisitic make an oral niche?

A

pH, salinity, temperature, presense of mineral and nutrients.

Niches changes all throughout life

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7
Q

surface level & accessible what kind of niche?

A

Temporary niche

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8
Q

What kind of niches

microbes form ECM ; Biofilms, less affected and difficult to penetrate

A

Permanent niches

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9
Q

When a baby is born with not teetht their mouth is a ?

A

Sterile space

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10
Q

First essential microbiome of the mouth and gut is Introduced by the mother’s ___ and ___

A

Skin and milk

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11
Q

What are the initial colonizer species in babys?

A

Good guys;
Strep Salivarius

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12
Q

The oral cavity is mainly invaded by ____ in the first year

A

Aerobes

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13
Q

All example of what kind of organims and when do they invade?

  • Streptococcus
  • Lactobacillus
  • Actinomyces
  • Neisseria
  • Veillonella

Study living and no vacation

A

Aerobes!
Initial invaders 1st year of life

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14
Q

Once tooth eruption begns, more ____ conditions can be found, these organisms can colonize on the nonshedding surfaces

A

Anerobic

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15
Q

Development of gingival crevices occurs for the colonization of what kind of microbs?

A

Periodontal microbs

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16
Q

what?

____ is seen at different sites on the tooth such as smooth surfaces and pit and fissure

A

Plaque accumulation

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17
Q

With aging when all teeth are lost, the flora becomes similar to what?

A

That of a child before teeth come in; aerobes.

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18
Q

In the battle of body agaisnt microbs;

What are things the body does to fight

A
  1. Physcial barries; regenerate mucousa
  2. New immune cells
  3. Nutriton & Oxygen (blood supply )
  4. Integrity of depper structures (bones, nerves)
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19
Q

In the battle of body agaisnt microbs;

What are things the microbs does to fight?

A
  • Finding mateirals (ex. iron)
  • Nutrients (ex. sugars)
  • Finding good niches
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20
Q

How to measure when attrition is hapening in the oral microbiom?

A
  • Probe for bone loss
  • Look for inflamammtion
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21
Q

Healthy biofilm on teeth, good micorbs in tisues.

A

Eubiosys

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22
Q

Some acidity, some destruction starting to occure over time. Lots of new niches

But still reversible

A

Dysbiosis

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23
Q

final destruction decay, not reversible, no going back to healthy balance, many niches .

A

Caries

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24
Q

healthy inflammation in tisue, required so immune systemic active.

A

Homeostatic inflammation

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25
Q

Characterisitc of a healthy oral microbiome include:

A
  • intact enamle
  • SUPRA-ginigval eubiotic biofilms
  • SUB-gingival eubiotic biofilms
  • Homeostatic inflammation
  • Inact Bone
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26
Q

Characterisitc of a diseased oral microbiome include:

A
  • Caries
  • Bone loss
  • Supra and sub ginigval dysbiotic biofilms
  • Periodontal pockets/ attachement loss
  • GCF on epithelium
  • Severe destructive inflmamtion
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27
Q

Dysbiosis .
bacteria that produce acid as they ferment sugars from the diet. The acid erodes the enamel of the teeth

A

Dental Cavities

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28
Q

In cavitys the frequency of acid production leads to what?

A

Erosion of the **buffering capacity of saliva **and frequent and sustained reductions in pH.

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29
Q

What 2 species of bacterial

metabolize dietary sugars to produce organic acids, primarily lactic acid.

The sustained production of these acids leads to a **drop in pH **in the oral environment, resulting in the dissolution of tooth mineral (demineralization).

A

Streptococcus mutans and lactobacilli

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30
Q

Main cariogenic bacteria

A

Streptococcus Mutans

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31
Q

What is the entry point for the gut microbiome?

A

The mouth

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32
Q

In a healthy mouth, the envioment is hostile to what kinds of organims?

A

Gram (-)

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33
Q

The acidity of the gut equalizes hostility following transtion from mouth, and becomes equally hostile to what organism?

In duodenum

A

Gram (+)

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34
Q

Beyond the duodenum what kind of organisms remain?

A

Gram (-) that survived the oral mucosa

Colonic bacteria

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35
Q

Is it good to see a lot of gram(–) anaerobes in the mouth?

A

NO. Colonic bacteria is very bad.

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36
Q

Benefits of oral microbiom

A
  • Prevents harmful colonization
  • maintians structural integrity of oral mucosa
  • produces vitamin K and some vit B
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37
Q

The presence of these commensal bacteria creates an environment that is unfavorable for the colonization of harmful pathogens.

A

Colonization Resistance

ensures that harmful bacteria have trouble establishing in the mouth

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38
Q

Every surface in the mouth, from the teeth to the tongue and cheeks, is covered with bacteria.

They occupy ____ for harmful bacteria

A

Binding sites

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39
Q

can create an environment where Opportunistic pathogens, like Candida (a type of fungus) or Staphylococcus aureus (a type of bacteria), can thrive and cause infections.

A

Antimicrobial Disruption

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40
Q

Some beneficial bacteria in the mouth produce substances that inhibit the growth of harmful bacteria.

This means they have ___ ____

A

Antagonistic Properties

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41
Q

This an example of?

Streptococcus salivarius, strain K12 produces a bacteriocin, a type of antimicrobial peptide, that can inhibit certain harmful esp Gram (-) bacteria associated with gum disease and bad breath.

A

Antagonistic Properties

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42
Q

Consumption of dietary nitrates that when consumed are reuduced to nitRITEs by oral bacteria.

Implicates caridovascular health.

A

Nitrate Metabolism

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43
Q

Overuse of H2O2 can do what?

A

Divert patient health, get rid of good bacteira and leave openings for harmful bacteria.

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44
Q

After ingestion, a portion of the dietary nitrate is secreted into the saliva and returned to the mouth. Here, oral bacteria convert it to **nitrite, which then enters the bloodstream**

A

Entero-salivary Circuit

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45
Q

A vasodialator, expands blood vessles, improves blood flow, reduces BP

A

Nitirc Oxide

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46
Q

Oral microbiome plays a crucial role in converting

A

Nitirates to nitrites

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47
Q

Usuing antimicorbial mouth rinses, can distrupt____ in the mouth

A

natural nitrate-reducing bacteria

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48
Q

Agressive oral health care routines can do what?

A
  • Disrupt beneficial bacteria in mouth
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49
Q

Consuming a diet high in sugars and carbohydrates can lead to frequent ____ in the mouth. This acid challenges the mouth’s natural pH balance and can weaken tooth enamel.

A

Acid production= lower pH= weaken tooth enamel

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50
Q

A Consistant acidic enviroment can causes what?

A

A shift in the oral micobiome, favoring bacteria that contribute to tooth decay

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51
Q

What are the 2 most complex microbioms in the body

A
  1. Colon
  2. Oral cavity
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52
Q

The ____ microbiota in the oral cavity plays a pivotal role in upholding oral and systemic health

A

Commensal

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53
Q

What diseases?

They arise from a complex interplay between the commensal microbiota, host susceptibility, and environmental factors.

Not imediate infection, they occur over time.

A

Caries and periodontitis

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54
Q

A lot of __ and ___ results ina microbiome that is able to withstand enviormental challanges

A

Redudancy & Diversity among microbs

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55
Q

Diseaaes that are associate with ____ microbiota are not causes by a single pathogen.

They arise from a combination of factors, including interactions with the commensal microbiota, host factors, and environmental influences.

A

Commensal Microbiota

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56
Q

a comprehensive resource that offers descriptions of oral bacterial taxa, a 16S rRNA identification tool, and a repository of oral bacterial genome sequences.

All bacteria have rRNA, useful tool for ID!

A

Human Oral Microbiome Database (HOMD)

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57
Q

In a healthy states, the microbiome is in a state of equillibrium.
Inbalances can result in an overgrowth of ?

A

Cariogenic Bacteria

58
Q

a type of biofilm, plays a crucial role in caries development.
Protects organims deep within the biofilm from antimicrobial agens.

Allow for accumulation of acid= demineralization

A

Dental plaque

59
Q

Allow for accumulation of acid in the mouth results in?

A

Demineralization of the enamel.

60
Q

WHat are some things that can result in further effects of cariogenic bacetria?

A
  • Frequent consumption of fermentable carbs
  • Reduce salivary flow; no neutalization of acids
61
Q

Regular oral hygiene practices, including brushing and flossing, can help

A

Remove dental plaque and # of cariogenic bacteria.

62
Q

What can lack of flossing and brushing result in?

Does it help to pick up healthy practices AFTER damage is done?

A

buildup of gram (-) anerobic bacteria that will form a niche and biofilm.

No.. Once biofilm is formed, then the water pick/brushing will not remove the plaque or decay.

63
Q

Prefers oxgen but can live without it

A

Facultative aerobes

64
Q

Requires oxygen to live

A

Obligative Aerobes

65
Q

Can only live in oxygen free enviroment, it cannot accept oxygen as its final electron acceptor.

A

Strict anerobes.

66
Q

In the layering of the microbes in the mouth, where do aerobes and anerobes sit?

A

Aerobes lay on top, oxygen rich

Anerobes lay deep below and populate the area ONLY after alll aerobes are down!

67
Q

Once Streptococcus Mutans enters the oral microbiome what will occur?

A

All other bacteria will be required to produce glucans, caries formation

68
Q

What is periodontal disease?

A

are infections of the structures around the teeth, which include the gums, periodontal ligament, and alveolar bone.

69
Q

What is the early stage of periodontal disease where the infection only affects the gums?

A

Gingivitis

70
Q

What is the primary cause of periodontal disease?

A

Accumulation of bacterial plaque on teeth and gums.

Smoking or chewing tobacco
Poor oral hygiene
Hormonal changes (e.g., pregnancy, menopause)
Diabetes
Medications that reduce saliva flow
Genetic susceptibility

71
Q

Symptoms of periodontal disease?

A

Red, swollen, or tender gums
Bleeding while brushing or flossing
Gums that pull away from the teeth
Persistent bad breath= anerobic bacteria
Loose or separating teeth
Changes in the way teeth fit together when biting
Formation of deep pockets between teeth and gums

72
Q

What is genetic susceptibility that live together and have the same diet that causes one person to have dental caries and the other not?

A
  • Epigenetics, gene for recessive or dominant trait differ in genes for how much enamel is produced over time others
  • differences in immune system function
  • microbiome makeup
73
Q

The most common form, characterized by inflammation within the supporting tissues of the teeth and progressive attachment and bone loss.

A

Chronic Periodontitis

74
Q

Occurs in patients who are otherwise clinically healthy and is characterized by rapid attachment loss and bone destruction.

A

Aggressive Periodontitis

75
Q

An infection characterized by the death of gingival tissues, periodontal ligament, and alveolar bone.

A

Necrotizing Periodontal Disease

76
Q

How do you treat Periodontitis?

A
  • SRP (deep cleaning)
  • Mediaction (antimicrobial rinsies, chips, gels)
  • Surgical tx (open flap, BG, tissue grafts)

Main goal = CONTROL INFECTION

77
Q

What are complications of periodontal disease

A
  • tooth loss
  • Increase risk of stroke, heart attack, etc.
78
Q

occur within the dental pulp and root canal system. They can lead to pain, abscess formation, and tooth loss.

A

Endodontic Infections

79
Q

Associated with what kind of infection?

Enterococcus faecalis + Fusobacterium nucleatum

A

Endodontic Infection

80
Q

How to prevent + treat endodontic infections?

A
  • RCT
  • Tx of caries
81
Q

characterized by an unpleasant odor from the mouth. It can be transient or chronic.

A

Halitosis (bad breath)

82
Q

Associated with what kind of infection?

Volatile sulfur compounds produced by bacteria, such as Porphyromonas gingivalis and Prevotella intermedia

A

Halitosis

83
Q

cancer can develop in any part of the oral cavity, including the lips, tongue, cheeks, and floor of the mouth

A

Oral Cancer

84
Q

How to avoid oral cancer?

A
  • Avoid tabacco & alcohol
  • regular dental check ups for early dectection
85
Q

What comesal bacteria?

Gram-positive bacterium.
Role in Health: Competes with S. mutans for colonization, thus potentially reducing the risk of cavities.
Distribution: Commonly found on tooth surfaces and in saliva.
Interactions: Can inhibit the growth of S. mutans by producing hydrogen peroxide.

A

Streptococcus sanguinis

Thank u next!

86
Q

What comesal bacteria?

Gram-positive bacterium.
Role in Health: Plays a role in the initial colonization of tooth surfaces, paving the way for other commensal bacteria.
Distribution: Found on tooth surfaces and in saliva.
Interactions: Forms biofilms with other oral bacteria, promoting oral health.

may bring bad bacteria later

A

Streptococcus gordonii

gordita gets to the buffet first

87
Q

What Pathogenic Bacteria?

Gram-negative bacterium.
Role in Disease: Associated with aggressive forms of periodontitis, especially in younger individuals.
Virulence Factors: Produces leukotoxin, which can damage white blood cells. Inhibit B cell growth.
Distribution: Found in the deep pockets (anerobic) of the gums in individuals with aggressive periodontitis

Juvinile periodontitis

A

Aggregatibacter actinomycetemcomitan

Star of the show!
Actino-my-see-com-it-tans

88
Q

What Pathogenic Bacteria?

Gram-positive bacterium.
Role in Disease: Along with S. mutans, it plays a significant role in dental caries.
Distribution: Found on tooth surfaces, especially in individuals with high cavity rates.

A

Streptococcus sobrinus

Need a straw cause theyre always drinking sodas with high cavity rates

89
Q

What Opportunistic Pathogen?

Gram-negative, anaerobicbacterium.
Role in Disease: Can contribute to periodontal disease, especially in pregnant women and smokers.
Distribution: Found in dental plaque and periodontal pockets.

weaker immune systems

A

Prevotella intermedia

Fake AF. Two faced. Play nice then strike when immune system is weaker

90
Q

What Opportunistic Pathogen?

Gram-negative bacterium.

Role in Disease: While typically harmless, it can cause infections in immunocompromised individuals.

Distribution: Commonly found in the oral cavity, especially in the presence of gum disease

A

Capnocytophaga

Nice, but They will fight you no cap! Doesnt care that youre weak.

91
Q

What bacteria has been found in elevated levels in oral cancer tissue?

promotes inflammtion + tissue damage

A

Peptostreptococcus anaerobius

92
Q

What does the mutans group of streptococcus produce?

A

They produce acid rapidly, which produces a more cariogenic enviroment.

Mutans, sorbinus, criceti

93
Q

What group of bacteria?

Found on mucosal surfaces, including the tongue.:

Streptococcus salivarius,
Streptococcus vestibularis

A

Salivarius group

94
Q

What bacteria from salivarius group?

Produces an extracellular unusual fructan but is not considered a significant opportunistic bacterium

A

Streptococcus salivarius

95
Q

What bacteria from salivarius group?

Generates urease, which produces ammonia, raising the local pH. Also produces hydrogen peroxide, contributing to the sialoperoxidase system.

Good bacteria. Combats mutans.

A

Streptococcus vestibularis

96
Q

Includes S. sanguinis, Streptococcus gordonii, Streptococcus mitis, and Streptococcus oralis.

Except for S. mitis, they may become opportunistic pathogens causinginfective endocarditis

A

Mitis Group

97
Q

WHat group?

Found in dental plaque and mucosal surfaces. Involved in various infections. Includes:
* Streptococcus anginosus
* Streptococcus intermedius
* and Streptococcus constellatus.

A

Anginosus Group

98
Q

An early adjacent colonizer of teeth. Shows “satellitism,” where growth is seen around colonies of other bacteria producing growth factors.

A

Granulicatella adiacens

99
Q

Includes
* Peptostreptococcus stomatis
* Parvimonas micra
* Finegoldia magna.

Often found in dental abscesses carious dentin, infected pulp chambers, root canals, and advanced periodontal disease.

A

Anaerobic Cocci

100
Q

Isolated from infected root canals and periodontal pockets resistant to therapy. Can survive bio-acids in the duodenum (gram +). HORRIBLE.

A

Enterococcus faecalis

101
Q

What Gram - cocci

Help in plaque formation by consuming oxygen, enabling obligate anaerobes to flourish.

A

Neisseria species

102
Q

What Gram - cocci?

Anaerobic Gram-negative cocci mostly found in plaque. They metabolize lactic acid.

A

Veillonella specie

103
Q

What gram - bacilli ?

Carbon dioxide-dependent, associated with destructive periodontal disease

A

Capnocytophaga:

104
Q

What gram - bacilli ?

Found in dental plaque, associated with various oral conditions.

A

Eikenella corrodens:

105
Q

What gram - bacilli ?

Strict anaerobes with a slender shape. Involved in inflammatory periodontal disease.

A

Fusobacterium species

106
Q

Smallest free-growing cells without a peptidoglycan layer. Found in saliva and oral mucosa. May play a role in salivary gland hypofunction and periodontal disease.

A

Mycoplasma Species

107
Q

anaerobic Dark oxygen deprived rods (colonic)

A

Gram Negative Rods

75% or more

108
Q

sunny aerobic cocci

A

Gram + Cocci

109
Q

viewed as harmless saprophytes. Their association with oral diseases is believed to be nutritional. Poor oral hygiene can lead to increased food debris and bacteria, which serve as food sources for these protozoa.

A

Protozoa in the oral microbiome

Entamoeba gingivalis and Trichomonas tenax

110
Q

What fungi?

are commonly found in the oral cavity. They are present in approximately half of the population and can exist without causing any symptoms.

Increase with age.

Albicans in main player

A

Candida Species
Cladosporium, Aureobasidium, Saccharomycetales, Aspergillus, Fusarium, and Cryptococcus.

111
Q

A healthy oral microbiome has what?

A

A lot of organims diversity

112
Q

When where is an overpowering amout of a certain bacteria in the mouth what does this mean?

A

Unhealthy microbiome

113
Q

4 characertistic of gingivitis

A
  1. Plaque accumulation
  2. Increased inflammation
  3. High GCF, low redox potential
  4. Mostly Gram (-) obligate anerobes
114
Q

WHat are the 7 key periodontopathogens?

A
  1. Porphyromonas gingivalis (red)
  2. Prevotella intermedia (orange)
  3. Tannerella forsythia (red)
  4. Aggregatibacter actinomycetemcomitans
  5. Fusobacterium nucleatum (orange)
  6. Capnocytophaga species
  7. Treponema denticola. (red)

Pigs Go Playing In The Fango And Act Funny Not Courteous So They dance

115
Q

In the sokransky tabel, green, purple, and yellow bacteria are what?

A

Host compatible and associated with healthy tissue sites in the mouth

116
Q

In the sokransky light & dark orange bacteria are what?

A

Are assocated with increased pocket depth and with higher amounts they leave to colonization of red complex bacteria

117
Q

In the sokransky red bacteria are what?

A

Found frequently in deep periodontal pockets,

118
Q

WHat 3 bacteria are found in red complex; which are found most frequently In deep periodontal pockets.

A
  • P gingivalis
  • T Forythia
  • T Denticola
119
Q

WHat 3 bacteria of the orane orange complex; which are found most frequently In deep periodontal pockets.

A
  • P. Intermedia
  • F nucleatum
  • Peptostreptococcus micros
120
Q

WHat 4 bacteria are found in green complex; Gram +

A
  • E corrodens
  • C gingivalis
  • C sputigene
  • C orchacea
  • C concisus
  • A actino a
121
Q

Agressive periodontitis is strongly associated with the presence of what tipping point bacteria that is good for prognostic measurements?

A

A actinomycetemcomitans

Amount is what matters, its can be in healthy sites. but high levels= ba

122
Q

Infection with this bacterium causes neutrophil abnormalities, including signal transduction, reduced chemotaxis, and phagocytosis, and increased superoxide production. Agresive pathogen. Escape adaptive immunity. Hard to kills.

A

A actinomycetemcomitans

123
Q

By A actinomycetemcomitans

Production of Collagenase and lipopolysaccharide(enzyme that destroy local tissue= make hard for adaptive repsosne) results in what effet?

A

activates the alternative complement pathway and stimulates** bone resorption.**

123
Q

A specific, anaerobic, polymicrobial infection of gingivaltissue by oral spirochetes (Treponema species) and a range of “fusiform” bacteria (Fusobacterium species), which together form a **“fusospirochetal complex”
MOST AGGRESSIVE. **

A

Nectrotizing Ulcerative Gingivitis

124
Q

is one of the red complex bacteria.

It is a Gram-negative, nonmotile, obligately anaerobic bacillus.

Use a broad range antibiotic

It **requires hemin **(which carries iron and protoporphyrin) and vitamin Κ for growth.

A

P gingivalis

125
Q

Once necrosis sets in what?

A

No antibiotics will help, you have clean the tissue and remove it.

126
Q

It is a Gram-negative, pleomorphic bacillus.

It is a strict anaerobe and requires vitamin K and hemin for growth.

It is associated with chronic periodontitis and dentoalveolar abscesses. Its virulence factors include phospholipase A, immunoglobulin (Ig) A/IgG proteases (more likely to stick, more likely to cause ilnesss) , mercaptans, and hydrogen sulfide.

A

P Intermedia

Orange Complex

127
Q

What virulence factor

revents opsinization Ex. Strep pneumonia

A

Capsul

128
Q

What virulence factor

Destroys neutrophils

A

Leukotoxins

129
Q

What virulence factor

destroy opsinization & Membrane attack complex

A

Complement degrading protease

130
Q

Explain plaque hypothesis

A

disease can be prevented by by interfering with inflammation, and altering the redox potential of the periodontal pocket to prevent the growth of obligate anaerobes.

131
Q

are pus-producing (pyogenic) infections associated with the teeth and surrounding supporting structures, such as the periodontium or alveolar bone, developes as extension of inital caries lesion

A

Dentaalveolar infection

132
Q

cocci arranged in chains or pairs, catalase negative

A

Strep Gram +

133
Q

Catalase postive

A

staphylococci

134
Q

streptococci form a **green zone **around their colonies as a result of incomplete lysis of red blood cells in the agar.

Partial destruction

A

Alpha Hemolysis

135
Q

streptococci form a clear zone around their colonies because complete lysis of the red cells occurs

Agressive

A

Beta Hemolytic

136
Q

zero hemolysis of RBC

A

Gamma Hemolytic

137
Q

the leading bacterial cause of pharyngitis and cellulitis (soft tissue infection

A

group A streptococcus (S. pyogenes)

138
Q

Partial hemolysis (alpha), green color.
Main cause of dental caries as mentioned before and are a major cause of endocarditis (once it reaches blood)

A

Viridans Group

S. pyogenes and S. agalactiae), S. pneumoniae and enterococci.
Strep.

139
Q

Treated with Penicillling G or Amoxicillian

A

Group A streptococcal infections

140
Q

Treated with either penicillin G or ampicillin

A

group B streptococcal infections

141
Q

In patients with damaged heart valves who undergo invasive dental procedures, endocarditis caused by viridans streptococci can be prevented by using what and when?

A

Amoxicillian PRE-OPERATIVLEY

PER 1001 (9 decks)

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