OCB03-2008 GLUTAMATE Flashcards
Why is a neuronal output described as an “integrated response”?
Determined by the NUMBER of excitatory and inhibitory synaptic inputs and the STRENGTH of each individual input
How can the timing of neuronal inputs affect the post-synaptic output?
Excitatory inputs close together = summation
Inhibitory inputs can dampen down the excitatory input/depolarisation if they occur simultaneously
What is glutamate and its importance in the nervous system?
Amino acid
Major excitatory neurotransmitter in the CNS
Describe some prominent glutamatergic pathways in the brain.
Cortico-cortical pathways - inflammation processing centres and modulation of neuronal activity
Between thalamus and cortex
Extrapyramidal pathway (cortex and striatum) - regulates involuntary movement
What psychiatric conditions are glutamatergic pathways implicated in? (4)
Depression
Anxiety
Schizophrenia
Drug addiction
What main functions is glutamate involved in? (3)
Memory
Emotion
Cognition
Can glutamate cross the blood-brain barrier?
No
How can glutamate synthesised in the CNS?
Metabolism of glucose (TCA cycle)
From glutamine in astrocytes
What is the concentration of glutamate in neuron cytoplasm?
1mM
What is the concentration of glutamate in presynaptic vesicles?
20-100mM
Describe the process of synaptic transmission in glutamatergic neurons.
AP depolarises neuron causing voltage-gated Ca channels to open = Ca++ influx
Exocytosis of presynaptic glutamate and diffusion across synapse to interact with postsynaptic receptors
Glutamate diffuses out of synaptic cleft and taken up by glutamate transporters on astrocytes
Describe the process of the recycling of glutamate after transmission.
Glutamate taken up into astrocytes by glutamate transporters
Glu –> Gln by glutamine synthase
Gln extruded by glutamine transporters into extracellular space
Gln taken up into neurons by glutamine transporters
Gln –> Glu by glutaminase
What is essential to terminate synaptic transmission via glutamate?
Uptake of Glu by astrocytes via glutamate transporters
What are the two main types of glutamate receptor?
Ionotropic
Metabotropic/7TMGPCR
Describe glutamate ionotropic receptors.
Fast transmission
Na+, K+, Ca+ allowed through (depolarise membrane)
4 sub-units
Post-synaptic