nucleotides + nucleic acids Flashcards
purine bases
adenine
guanine
pyramidine
cytosine
uracil
thymine
DNA
deoxyribose
A T C G bases - carbon 3
phosphate group - carbon 5
double stranded - antiparallel strands in a helix wound around histones
1 type of DNA
RNA
ribose
A U C G bases - carbon 3
phosphate group - carbon 5
single stranded
3 types - mRNA, tRNA, rRNA
sugar phosphate backbone
between phosphate at carbon 5 of pentode sugar and hydroxyl group on carbon 3 of the pentode sugar of adjacent nucleotide. Nucleotides joined by phosphodiester bonds
semi conservative DNA replication
- DNA helicase breaks H bonds between bases - leaving two single strands of DNA with exposed bases
- Free nucleotides bind to exposed bases by complementary base pairing
- DNA polymerase catalyses formation of phosphodiester bonds between adjacent nucleotides
- DNA ligase seals strand
why DNA replication
- eliminates/ detects mutations
- conserves genetic information accurately through generations
gene
section of DNA which codes for a specific protein
features of genetic code
- triplet
3 bases = 1 codon, every codon codes for an amino acid - degenerate
amino acids have more than 1 codon - universal
all organisms use same code, codons code for same amino acid - non-overlapping
each base part of only 1 codon (read in 3’s)
transcription
- DNA helicase unzips section of DNA containing gene by breaking H bonds
- sequence on antisense strand used to produce mRNA molecule
- free RNA nucleotides join your exposed bases on template strand by complementary base pairing
- RNA polymerase catalysed the formation of phosphodiester bonds between adjacent nucleotides
- Completed mRNA molecules leaves nucleus’s via pores
translation
- mRNA molecules binds to subunit on ribosome
- start codon read, tRNA molecule carrying amino acid with complementary anticodon binds to codon
- repeats
- peptidyl transferase catalyses formation of peptide bonds between adjacent amino acids forming a primary protein structure
- chain folds into secondary and tertiary structure