cell components Flashcards
light microscope
magnification
resolution
mag - x2000
res - 200nm
electron microscope
magnification
resolution
mag - x500,000
res - 0.3-10 nm
scanning electron microscope
image
magnification
resolution
3D image
mag- x200,000
res - 3-10nm
transmission electron microscope
image
magnification
resolution
2D image black and white
mag - x2,000,000
res - 0.5nm
resolution
ability to distinguish between 2 points and view individual objects as separate entities
magnification
how much bigger an object appears compared to the original object
fluorescence
the absorption and re-radiation of light
sample preparation method
depends on…
1)
2)
1) nature of specimen
2) resolution
why differential staining?
- Creates contrast
- Highlights specific features in cell
- Highlights structures/ cells
- Produces a better/ clearer image
cells
basic unit of living matter
ultrastructure
fine structure within a cell, viewed at high magnifications