Nucleic Acids and Proteins Flashcards
Biomacromolecules
large molecules made up of living organisms
Polymer
Made up of macromolecules and repeating subunits
Polymer examples
- polysaccharide
- polypeptide
- nucleic acid
Monomer
molecule that forms the basic unit for polymers
Monomer examples
- monosaccharide
- amino acid
- nucleotide
Condensation polymerisation
Monomer to polymer, releases water
Hydrolysis
polymer to monomer, requires water
Functions of Proteins
speeds up chemical reactions, cell recognition, cell communication, movement
Proteome
Complete set of proteins expressed by the genome of an individual
Proteomics
study of structure, functions and interactions of proteins
Protein Synthesis
- Primary Structure
- Secondary Structure
- Tertiary Structure
- Quaternary Structure
Primary Strucure
Sequence of amino acids. Monomers are joined by anabolic reactions which require energy
Secondary Structure
Folding of primary structures into alpha helices or beta pleated sheets
Tertiary Structure
the 3D shape of the protein determines its function.
hydrogen bonds, ionic bonds, covalent bonds, hydrophobic interactions
Quaternary Structure
2 or more polypeptide chains joined to form a functional protein
Nucleic Acids
responsible for carrying all genetic information of organisms and encodes the information for synthesis of protein in cells
Bases
Nitrogen,
Purines - Adenine and Guanine
Pyrimidines - Cytosine, Thymine, Uracil
DNA - deoxyribonucleic acid
Makes up genetic code, carries instructions for making proteins.
Strands run antiparallel, when nucleotide is added can only be added to 3’ end
RNA - ribonucleic acid
Involved in every stage on gene expression.
transferRNA, ribosomalRNA, messengerRNA
tRNA
Transfers amino acids from the cytosol to a ribosome
rRNA
makes part of the ribosome, the site of translation
mRNA
carries a transcription of genetic sequences to a ribosome for translation
Gene expression
the process of transcribing and translating a genetic sequence into a gene product
Triplet
a sequence of 3 DNA bases
Codon
sequence of 3 mRNA bases
Anticodon
sequence of tRNA bases
Transcription
to copy a template strand of DNA into mRNA to carry instructions for a polypeptide chain to a ribosome
Steps of transcription
DNA template > mRNA > polypeptide
- DNA partially unwinds, Helicase and Gyrase
- RNA primer binds at starting point
- RNA polymerase codes for complimentary strand of RNA
Steps for Translation
mRNA > protein
- mRNA arrives at ribosome
- ribosome heads group of 3 nucleotides
- tRNA has specific anticodon complimentary to codons of mRNA
- peptide bond forms between joining amino acid, tRNA removed to be used again
- polypeptide production stops when “stop” sequence is reached