Nucleic acids Flashcards
What does adenine bond with?
thymine
What does guanine bond with?
cytosine
What causes the complimentary base pairings?
size of the nucleotides
hydrogen bonding between bases
What does the number of purines equal?
number of pyrimidines
What are purines?
adenine
guanine
How many rings so purines have?
double ringed
What are pyrimidines?
thymine
cytosine
How many rings do pyrimidines have?
single ring
When does the replication of DNA occur?
S phase of cell cycle interphase
How does DNA replicate?
-The enzyme helicase unzips the DNA (breaks hydrogen bonds)
-SSB (single stranded binging) proteins bind to DNA strands
- DNA polymerase (an enzyme) makes new DNA from deoxyribonucleotides
-Primase makes the primer that tells polymerase where to work
Ligase repairs any breaks in the sugar phosphate backbone
What direction can DNA polymerase work?
5’ (prime) to 3’ (prime)
What is the strand that goes in the 5’ to 3’ direction called?
Leading strand
What is the strand that goes from 3’ to 5’ called?
lagging strand
Why is the lagging strand slow?
Because polymers has to put down more primers
What are Okazaki fragments?
Fragments of synthesized DNA on lagging strand
What are primers made of?
RNA
Why is DNA replication known as semi-conservetive?
Because each new DNA contains one old strand and one new one
What is a gene?
The segment of DNA that specifies a specific amino acid sequence