Nuclear & Cytoplasmic Staining Flashcards
1
Q
Vocab
Auxochrome
A
The chemical group present in a dye that enables the dye to link firmly to tissue. The group is usually charged, so the bond is concidered ionic.
Ex:
-Amino NH2
-Carboxyl COOH
-Sulfonic SO3
-Hydroxyl OH
2
Q
Connections
Why bother using Methyl green-pyronin Y?
A
Purpose for stain method is distinguish DNA vs RNA nucleic acids and indentify plasma cells or immunoblasts in tissues.
3
Q
Results
What should be the staining pattern seen after a methyl green-pyronin Y treatment?
A
DNA= Green to blue-green (methly green)
Nuclei = Green to blue green
RNA= Red to rose (pyronin)
Goblet cells= Mint Green
Immunoblasts & plasma cell cytoplasm= Intense red
