Nerve

Question 1

What is th purpose of the Bielschowsky-periodic acid-Shiff (PAS) stain?

From Self Instruction textbook, notes & etc

Answer 1

To demonstrate nerve fibers, and the presence of neurofibrillary tangles and senile plaques in Alzheimer disease.

Question 2

What does the Schiff reaction do in the Bielschowsky-periodic acid-Shiff stain?

From Self Instruction textbook, notes & etc

Answer 2

Stains both the basement membranes & amyloid in the plaques.

Question 3

What does sodium thiosulfate remove in the Bielschowsky-periodic acid-Shiff stain protocol?

From Self Instruction textbook, notes & etc

Answer 3

Sodium thiosulfate removes any unreduced silver

Connect: Gram/Lugol idoine & solidum thiosulfate remove mercuric pigment

Question 4

What is the reducing agent used in the Bielschowsky-periodic acid-Shiff stain protocol?

From Self Instruction textbook, notes & etc

Answer 4

Formaldehyde

See the developer reagent solution