Cytopreparatory Techniques Flashcards
How many dyes and how many solutions make up the Papanicolaou (Pap) stain protocol?
From Self Instruction textbook, notes & etc
Five dyes: Hematoxylin, orange G, light green SF yellowish, eosin Y, & Bismarck bown Y
3 Solutions: Gill (or Hariss) Hematoxylin, OG-6, & EA
The last two solutions have phosphotungstic acid as mordants
EA solutions used in cytologic staining contain:
A. fast green, eosin Y and phosphomolybdic acid
B. Orange G, phosphomolybdic acid, and eosin Y
C. Light green, orange G, and phosphomolybdic acid
D. Light green, eosin Y, and phosphotungstic acid
BOC 2021 HTL Question Pool
D. Light green, eosin Y, and phosphotungstic acid
EA solutions contain light green, eosin Y, and phosphotungstic acid. Some formulas also contain Bismarck brown.
What is the purpose of the Pap stain?
From Self Instruction textbook, notes & etc
To distinguish cellular components by obtaining highly detailed chromatin, differential counterstaining, and cytoplasmic transparency.
The differential counterstaining of cells at diff metabolic states
What cellular components do each of the three solutions in the Pap stain demonstrate?
From Self Instruction textbook, notes & etc
Hematoxylin [Blue]: Crisp & distint nuclear detail
OG-6 [Orange]: tonofilaments of keratin (distinguish keratinizing neoplasms)
EA: Cytoplasm spectrum of color
-Metabolically inactive cells= pinkish
–>Eosin stains superficail squamous cells, nucleoli, erithrocytes, & cilia
-Metabolically active cells= blue-greenish
–>light green stains intermedial squamous, parabasal & columnar cells; histiocytes, leukeocytes, large & small cell undifferentiated carcinomas & adenocarcinomas
Phosphotungstic acid acts as a dye excluder in EA
Troubleshooting
A Papanicolaou stained smear shows only blue-green cytoplasm in all cells. No pink cytoplasm is noted.
From Self Instruction textbook, notes & etc
The cytology prepartory tech should be cautione about:
1. Over-rinsing the PAP slides
2. Staining in EA for too short a time
