Enzyme Histochemistry

Question 1

Acid phosphatase

Answer 1

-considered a marker enzyme for lysosomes
-stain indicates presence of inflammatory cells a biopsy frozen sections
!Sodium barbital used in procedure is a narcotic, harmful if swallowed, inhaled, or eye contacted!

Question 2

NADH diaphorase

Answer 2

Technique that demonstrates abnormalalities in mitochondria, Z-band material, & sarcoplasmic reticulum on frozen section samples.
-pH critical
-NAD+ may become unstable
-diformazan-protein complexes can be observed/mistaken to mitochondria

Question 3

Succinic dehydrogenase (SDH)

Answer 3

Further ID source of NADH diaphorase activity, because only mitochondria show positive rxn. As SDH is a mitochondrial enzyme and participates in the Kerb cycle, so it’s a useful index of the actvity of the cycle.