Normal Hemostasis - Coag Cascade Flashcards
Factor 1/ FI
also a?
increased with?
high levels associated with?
dysfucntional w?
AFR
increased w inflammation/pregnancy/smokers
high fibrinogen assoc w bleeding
dysfuctional w thrombosis
FII
prothrombin
Vit K dependent
one of magic 4
FIII - TFIII
cofactor or activation of?
released from injured cells
cofactor for activation of FVII
no known deficiency
FIV
mediates?
low #
in coag screen?
calcium
mediates plt adhesion
low number not a problem in vivo
in coag screen must add calcium to overcome anticoag
FV
can attach?
inactivated by?
LABILE
can attach to receptors
inactivated by protein C
FVI
doesnt exist
FVII
what kind?
activated by?
Stable factor
Vit K dependent
magic 4
activated by FFIII/ Calcium
FVIII
what complex?
deficiency?
unstable when?
what portion?
von willebrand complex
deficiency = hemophilia A
unstable when not bound to VWF
coagulant/clotable portion
FIX
deficiency?
christmas factor
Vit K dependent
magic 4
deficiency Hemophilia B
F X
what complex
magic 4
prothrombin complex
stuart factor
F XI
what kind?
deficiency?
complexes with?
contact factor activated by glass
Def causes hemophilia C
complex with HMWK
F XII
another name?
what kind?
only factor?
hageman
contact factor activated w glass
activates XI w HMWK/PK and kalikrein
ONLY FACTOR DEF THAT DOESNT CAUSE BLEEDING
F XIII
also called
acts by
bonds where?
not? MUST
fibrin stabilizing factor
act by thrombin/calcium
covalent bonds between D domains in fibrin
Not tested for in coag screen, MUST detect a def w UREA CLOT LYSIS
PK
contact factor
does not cause bleeding w defciencies
HMWK
contact factor
doesnt cause bleeding
Bradykinins
increase
contract
dilate
induce
increase vasc perm
contract smooth muscle
dialate BV
induce inflammation
Healthy clot
fibrinogen, XIII, VWF
NOT EDTA
degrades factor V
NOT heparin
inhibits?
inhibits coag factors
No hemolyzed blood
what happens in rbcs?
erythrocetin in rbcs has thromoplastic effect
Handling
glass can activate coag cascade, use PLASTIC
Sample storage
fridge for?
what rt?
freeze at? and then
Fridge for 4hr
PT room temp
freeze at -20c or colder rapidly and thaw rapidly
What can IIa convert?
can convert I - Ia
thrombin
Factor X activation
requires activation of complex
tenase complex
intrinsic and extrinsic
Factor II (prothrombin) activation
requires prothrominase complex
The Magic Four
all located in
all dependent on
all depleated by
II, VII, IX, X
all in liver
all Vit K dependent - adds second carboxyl group to complete factor
All depleated by warfarin therapy (keeps vit K in storage form)
Thrombin is the only factor to
convert fibrinogen I to fibrin Ia
enhances V, VIII
Factor V/VIII
short
not?
able to ?
targets?
activated by?
Labile factors short lived
not enzymes - cofactors
able to particpate in coag inactive form
targets protein C
activated by IIa
Thrombin IIa
acts on fibrinogen I , splits
fibrinopeptides A and B form visible solid clot
w calcium activates XIII
Plasminogen to plasmin
important for fibrinolysis
when Xa is activated - pk converts to kalikrein to plasminogen to plasma
Kalikren converts HMWK to bradykinins
