Nonenzymatic Protein Function and Protein Analysis Flashcards
characteristic trihelical fiber and makes up most of the extracellular matrix of connective tissue
Collagen
repetitive organization of secondary structural elements together
motif
important component of ECM of connective tissue, stretch and recoil like a spring
elastin
intermediate filament proteins found in epithelial cells
Keratins
protein that makes up microfilaments and the thin filaments in myofibrils
Actin
protein that makes up microtubules
Tubulin
primary motor protein that interacts with actin, thick filament in myofibrils and involved in cellular transport
Myosin
motor proteins associated with microtubules
Kinesin and dyneins
proteins found on the surface of most cells and aid in the binding the cell to the ECM other cells
Cell Adhesion Molecules CAMs
glycoproteins that mediate Ca-dependent cell adhesion
Cadherins
proteins that have 2 membrane spanning chains alpha and beta. Important for binding and communicating with ECM. Play very important role in cell signaling
Integrins
bind to carbohydrate molecules that project from other cell surfaces
selectins
process in which cells receive and act on signals
biosignaling
have no gates and are therefore unregulated
ungated channels
gate is regulated by the membrane potential change near the channel
voltage-gated channels
binding of a specific substance or ligand to the channel causes it to open or close
ligand-gated channels
anchors the receptor in the cell membrane
membrane- spanning domain
stimulated by the appropriate ligand and induces a conformational change
ligand-binding domain
activated by the conformational change. Often results in the initiation of a second messenger cascade
catalytic domain
large family of integral membrane proteins involves in signal transduction
G protein-coupled receptors (GPCR)
separates proteins on the basis of mass alone
SDS-PAGE
separating proteins on the basis of their isoelectric points
Isoelectric focusing
uses physical and chemical properties to separate and identify compounds from a complex mixture
Chromatography
