Nitric Oxide : GPCR

Question 1

The only free radical, that is beneficial____?

Answer 1

Nitric oxide

Question 2

NO act as a?

Answer 2

Neurotransmitter

Question 3

Responsible for?

Answer 3

Vasodilation,
Smooth muscle relaxation
Immune response

Question 4

Also referred as a _____?

Answer 4

Endothelial derived relaxation afctor

Question 5

NO produce in _____ ?

Answer 5

Epithelial Cells

Question 6

Recepyor of NO?

Answer 6

receptor guanylate cyclases (RGCs)

Question 7

RGC presents in

Answer 7

Vascular smooth muscle at heme 1 molecule of catalytic site of RGC

Question 8

Binding of NO with RGC_______

Answer 8

activates the Guanylyl cyclase activity, which results in production of cGMP.

Question 9

NO produce By _____ ?

Answer 9

NO synthases

Question 10

Steps for activating endothelial NO Synthase (eNOS)

Answer 10

Acetylcholine binds at GPCR
Gq
PLC
PILP2
IP3 + DAG
IP3 binds at ER
Release of Ca ion in cytosol
Ca ion increases
Ca ion binds with calmodulin
activate eNOS

Question 11

eNOS?

Answer 11

an enzyme which is responsible for producing NO from amino acid L-arginine.

Question 12

eNOS + L-Arginine ——-> ?

Answer 12

NO (gas) + Citrulline

Question 13

NO can diffuse out of?

Answer 13

Endothelial cells

Question 14

NO can diffuse into ?

Answer 14

Adjacent smooth cells of Blood vessels

Question 15

In Smooth muscle cells,NO Binds with?

Answer 15

heme group of soluble Guanylate cyclase (sGC)

sGC get activated

GDP to GMP conversion

cGMP level increases

Question 16

cGMP activates ?

Answer 16

PKG

Question 17

PKG promotes?

Answer 17

Decrease in the sensitivity of contractile machinery

Relaxation of smooth muscles by reducing ca ion level

Question 18

Reducing calcium level leads to ?

Answer 18

Relaxation of Blood vessels (Vasodilation)

Increases Blood flow

Reduces BP

Question 19

Half life of NO in?

Answer 19

seconds

Question 20

Inhibitors of PDE5?

Answer 20

Sildenafil citrate(Viagra)

Question 21

what happens by inhibiting PDE5?

Answer 21

Continuous vasodilation will be observed

Question 22

Cells that can produce NOS?

Answer 22

Neurons (Neurotransmitter)

Immune cells (Phagocytice cells)

Endothelial cells (Vasodilation)