Nitric Oxide : GPCR Flashcards
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The only free radical, that is beneficial____?
Nitric oxide
NO act as a?
Neurotransmitter
Responsible for?
Vasodilation,
Smooth muscle relaxation
Immune response
Also referred as a _____?
Endothelial derived relaxation afctor
NO produce in _____ ?
Epithelial Cells
Recepyor of NO?
receptor guanylate cyclases (RGCs)
RGC presents in
Vascular smooth muscle at heme 1 molecule of catalytic site of RGC
Binding of NO with RGC_______
activates the Guanylyl cyclase activity, which results in production of cGMP.
NO produce By _____ ?
NO synthases
Steps for activating endothelial NO Synthase (eNOS)
Acetylcholine binds at GPCR
Gq
PLC
PILP2
IP3 + DAG
IP3 binds at ER
Release of Ca ion in cytosol
Ca ion increases
Ca ion binds with calmodulin
activate eNOS
eNOS?
an enzyme which is responsible for producing NO from amino acid L-arginine.
eNOS + L-Arginine ——-> ?
NO (gas) + Citrulline
NO can diffuse out of?
Endothelial cells
NO can diffuse into ?
Adjacent smooth cells of Blood vessels
In Smooth muscle cells,NO Binds with?
heme group of soluble Guanylate cyclase (sGC)
sGC get activated
GDP to GMP conversion
cGMP level increases
cGMP activates ?
PKG
PKG promotes?
Decrease in the sensitivity of contractile machinery
Relaxation of smooth muscles by reducing ca ion level
Reducing calcium level leads to ?
Relaxation of Blood vessels (Vasodilation)
Increases Blood flow
Reduces BP
Half life of NO in?
seconds
Inhibitors of PDE5?
Sildenafil citrate(Viagra)
what happens by inhibiting PDE5?
Continuous vasodilation will be observed
Cells that can produce NOS?
Neurons (Neurotransmitter)
Immune cells (Phagocytice cells)
Endothelial cells (Vasodilation)
