new bio Flashcards
The primer that is binding to a DNA sequence is made of ___.
RNA
DNA polymerase can/can’t start without a 3’ OH already there.
can’t
What part of DNA gives it a charge?
Phosphate
What is one molecule that is found in protein but NOT DNA?
Sulfur (disulfide bond in tertiary structure)
In the experiment with radioactive viruses, where was the radioactive DNA found? Where was the radioactive protein?
Radioactive DNA: inside cells, in pellet
—> confirms that genes are made of DNA
Radioactive protein: inside solution
—> confirms capsids are made of protein
Which of the following best describes the composition of DNA monomers?
a) All of the monomers contain ribose connected to one of four nitrogenous bases
b) All of the monomers contain a phosphate group connected to a nitrogenous base
c) All of the monomers contain a phosphate group and a nitrogenous base connected to a deoxyribose.
C
5 prime end is characterized by a ___ and the 3 prime is characterized by an ___ group.
phosphate, OH
In DNA, what type of bond is between the bases?
Van der Waals
DNA replication is semiconservative because replicated DNA molecules are composed of ___.
a) 2 strands of old DNA
b) 2 strands of new DNA
c) 1 strand of old DNA and 1 strand of new DNA
d) 1 strand of new DNA
C
What did the Meselson-Stahl Experiment prove?
DNA replication is semi-conservative
Meselson-Stahl Experiment results proving semi-conservative replication.
After two generations, 1/2 of DNA was low density and the other 1/2 was intermediate density.
DNA polymerase catalyzes DNA ___.
synthesis
Deoxyribonucleotides are only added to the __’ end of a growing DNA chain. Synthesis always from ___ to ___ prime
3’, 5 to 3
DNA synthesis requires energy, making it ___.
endergonic
Why is ATP a good source of energy?
Three phosphates that are all negatively charged want to repel and release a lot of energy.
