Chapter 4-7 Biology Flashcards
Nucleic acid monomers
Nucleic acids are made up of nucleotides
DNA has deoxyribonucleotides
RNA has ribonucleotides
Nucleic acid polymers?
DNA and RNA are both nucleic acid polymers
DNA= deoxyribonucleic acid
RNA= ribonucleic acid
Primary structure of DNA
Linear sequence of nucleotides/nitrogenous bases (ATGC) linked together via phosphodiester bonds
Secondary structure of DNA
Double- helix, hydrogen bonding between the bases, antiparallel strands
Tertiary structure of DNA
Supercoils, wrapping around histones
What are all the bases of DNA and how do they pair?
Adenine - Thymine
Guanine - Cytosine
What are all the bases of RNA and how do they pair?
Adenine - Uracil
Guanine - Cytosine
Which bases are pyrimidines and which ones are purines? (Remember shorter name longer structure)
Pyrimidines: cytosine, uracil, and thymine
Purines: guanine, adenine
4 differences between DNA and RNA
- The sugar-phosphate backbone in DNA is deoxyribose and RNA’s sugar is ribose (Primary structure diff.)
- RNA has uracil instead of thymine (Primary structure diff.)
- RNA is single-stranded and DNA is double-stranded (Secondary structure), therefore making RNA less stable
- The OH group on ribose is much more reactive than the H in deoxyribose
Primary structure of RNA
Linear structure of bases, extending from a sugar-phosphate backbone
Secondary structure of RNA
Double helical stem and unpaired loop
Tertiary Structure of RNA
Pseudoknots and more complex, weird loops
Three components of nucleotides
- Phosphate group
- Five carbon sugar
- Nitrogenous base
Between RNA and DNA which has the sugar with a hydroxyl group (OH) on the 2’?
RNA has the OH, DNA just has an H (hence the DEOXY…)
The relation between amino acid and polypeptide is similar to the relationship between
a) phospholipid and plasma membrane
b) nucleotide and nucleic acid
c) glycogen and glucose
d) triglycerides and steroids
b
How do nucleotides polymerize to form nucleic acids? What bonds form between nucleotides?
Condensation reaction; phosphodiester bond, a covalent bond between the sugar of one nucleotide and the phosphate of a second
How is the double helix stabilized?
- Hydrogen bonding between the base pairs
- Hydrophobic interactions (what causes the DNA to twist)
- Van der Waals interactions (what forms base stacking)
Which bases pairs have three hydrogen bonds? (Hint: think about how which base pairs are have a faithful marriage when in DNA and RNA)
Guanine and Cytosine.
Adenine (the cheater) has two bonds with both thymine and uracil.
Which RNA structure will form spontaneously?
Secondary
In RNA’s secondary and tertiary structures, the bases participating in hydrogen bonding are ___.
antiparallel
Carbohydrate monomers
monosaccharides
Oligosaccharides = __ sugars
Few
Polysaccharides = __ sugars
Many
4 ways carbohydrates differ
- Aldose or ketone placement of the carbonyl group
- Number of carbon
- Different arrangement of hydroxyl groups in space
- Linear and alternative ring forms
What is the carbohydrate molecular formula?
(CH2O)n
n= # carbon hydrates
ranges 3=1000+
Where is the carbonyl group located on an aldose?
The end of a carbon chain
Where is the carbonyl on a ketose?
Within the carbon chain
___ can be used as a source of carbon atoms or energy. ___ must be converted to (first blank) first.
Glucose, Galactose, Glucose
Phosphodiester is between __’ phosphate group and __’ hydroxyl group
5, 3
N-terminus vs C-terminus
N has amino
C has carboxyl
Purines will always bind to ___
pyrimidines
Steps in DNA replication
- Strand separation (breaking of hydrogen bonds)
- Complementary base pairing
- Polymerization
True or False: RNA is more diverse in size and shape compared to DNA
True
What is the function of a ribozyme
To catalyze reactions (have active sites like proteins)
Carbohydrates would be considered
a) polar
b) nonpolar
c) hydrophobic
d) hydrophilic
a and d
5 types of carbohydrates
- Starch
- Cellulose
- Glycogen
- Chitin
- Peptidoglycan
OH placement on glucose and galactose
glucose: behind plane
galactose: above plane
The predominant form of glucose dissolved in an aqueous solution is __.
the ring configuration
Aldose and ketone are both __
carbonyls
Pentose is in ___.
DNA
What are two things you can look at to distinguish carbohydrates?
- # of carbon atoms
- OH orientation
Monosaccharides polymerize via ___ reaction and form ___ linkages
condensation, glycosidic
2 most common glycosidic linkages
- alpha 1-4
- beta 1-4
1 and 4 refers to the C-1 and c-4
A glycosidic linkage is analogous to __ in proteins and ___ in nucleic acids
Peptide, phosphodiester
5 types of polysaccharides
- Starch
- Glycogen
- Cellulose
- Chitin
- Peptidoglycan
Starch is energy storage in ___ cells. and has __ glucose monomers, forming an ___ helix
plant, alpha, alpha
Amylose has an alpha-_ glycosidic linkage and amylopectin has an alpha 1-_ glycosidic linkage. Which one has a branching structure?
4, 6, amylopectin
Glycogen is energy storage in __ and it’s stored in the __ and __.
animals, liver, muscle
Glycogen can be broken into __ monomers for energy and has a ___ alpha glucose polymer (1-6).
glucose, branched
Cellulose is a ___ polymer and has a __ 1-4 glycosidic linkage. It has __ bonds between adjacent parallel strands.
structural, beta, hydrogen
Cellulose is a major component of the __ __ in plant cells.
cell wall
True or false: Cellulose is not a linear molecule
False, it is linear and looks like a ladder (hydrogen bonds are the rungs)
Similar to cellulose, chitin is a ___ polymer.
structural
Chitin is a major component of the cell wall of __ and the exoskeleton for __ and __.
fungi, insects, crustaceans
Chitin has a beta __ glycosidic linkage and it has hydrogen bonds between ___ parallel strands.
1-4, adjacent
Peptidoglycan is found in __ cell walls.
bacterial
All of the structural carbohydrates have __ structures.
linear
Peptidoglycan has a __ 1-4 glycosidic linkage and ___ bonds between adjacent strands (make it a bit stronger).
beta, peptide
Glycosidic bonds form between what groups?
hydroxyl
Why can animals easily degrade starch, but not cellulose?
Animals can degrade alpha 1-4 but not beta 1-4
Cellulose, chitin, and peptidoglycan form short/long strands.
long
4 functions of carbohydrates
- Precursors for many different types of molecules
- Structural support
- Cell identity
- Storage of chemical energy
Two reasons why beta 1-4 glycosidic linkages do NOT hydrolyze easily
- Most organisms lack the enzymes to hydrolyze them
- Fibers exclude water, making hydrolysis difficult
2 structures important for cell identity function
- Glycoproteins- protein+carb
- Glycolipids- lipid+carb
What is cell-to-cell recognition?
When cells can identify themselves
Cell-cell signaling
communication b/w cells
C-O bonds are held tightly and have high/low potential energy.
C-H/C-C bonds are weaker and have high/low potential energy.
low, high
3 types of lipids
- Fats
- Steroids
- Phospholipids
All three types of lipids found in cells are insoluble in water because they all possess __
a) fatty acids
b) many ester linkages
c) lots of hydrocarbons
c
Why is a plasma membrane necessary?
- keeps all organelles inside the cell
- facilitates what goes in and out the cell
- important in chemical reactions
- creates different concentration gradients
What are key parts of lipid structure?
- Carbon containing compounds
- HYDROCARBONS
- Nonpolar C-C and C-H
VERY hydrophobic
Fatty acids are a hydrocarbon chain with around __ to __ carbon atoms and a ___ functional group.
14, 20, carboxyl
What are the two types of fatty acids, which one has the double bond?
- Unsaturated, has the double bond
- Saturated
What can the double bond create in unsaturated fatty acids?
Kinks, meaning unsaturated fatty acids can be polyunsaturated
Three S’s: Saturated, ___, and ___
single (bonds), solid (because long tails are stiff)
Which ones has the max amount of Hydrogens, unsaturated or saturated?
Saturated
Unsaturated bonds have long or short bonds?
short
Steroids have a bulky __ __ structure.
four-ring