Neuroanatomy + Electrophisiology Techniques

Question 0

What is microiontophoresis?

Answer 0

Use of a multi-barrelled electrode to simultaneously record and apply different drugs. (Used in extracellular current-clamp)

Question 1

Current clamp recording measures___?

Answer 1

Voltage (membrane potential)

Question 2

What recording technique is useful for small neurone recordings and where the electrode is sealed to membrane?

Answer 2

Patch-clamp

Question 3

What recording technique is used for large cells?

Answer 3

Two electrode voltage-clamp

Question 4

What does optogenetics involve?

Answer 4

Introduction of foreign LIGHT-ACTIVATED ion channels into a group of neurones

Question 5

What is DAPI stain used for?

Answer 5

Detect DNA in living and fixated neurones

Question 6

What stain identifies degenerating axons?

Answer 6

Nauta Silver stain

Question 7

Advantage of Golgi stain is?

Disadvantage is?

Answer 7

Labels cell body and NEURITES

Only small % of neurones in sample labelled

Question 8

What is the resolution of an electron microscope?

Answer 8

0.1 nm

Question 9

Immunohistichemistry uses _______ to visualise ______?

Answer 9

Antibodies

Proteins (peptide NTs)

Question 10

Fast axoplasmic transport = ___ mm per day.

Slow axoplasmic transport = ___ mm per day.

Answer 10

1000 mm

1-10 mm

Question 11

ANTEROGRADE axoplasmic transport is movement from _____ to _____?

Answer 11

Soma to axon terminals

Question 12

Advantages of transneuronal tracing:

Answer 12

Shows whole neuronal circuit

Tracer can cross synapses