Neisseria and Moraxella Catarrhalis Flashcards
What are the characteristics and gram of Neisseria?
GNC(usually in pairs), aerobic, oxidase and catalase positive
What are the characteristics and gram of Moraxella catarrhalis?
GNC(other Moraxella are rod shaped), graywhite colonies, “hockeypuck” colonies
Where is Neisseria found?
Mucus membranes of man, normal oroparyngeal flora
Where is Moraxella found?
normal oroparyngeal flora
What are the growth requirements for pathogenic species?
fastidious
What is the purpose of the candle jar
CO2 environment
What is positive catarrhalis disk?
green
What is ONPG testing for?
late utilization of glucose
What are the 4 carbohydrates?
Glucose (dextrose), maltose, lactose and sucrose
What is superoxol?
Same as catalase just use 30% instead of 3% H2O2
G+, M-, L-, S-; What is this bacteria?
N. gonorrhoeae
G+, M+, L-, S-; What is this bacteria?
N. meingitidis
G+, M+, L+, S-; What is this bacteria?
N. lactamica
G-, M-, L-, S-; What is this bacteria?
N. catarrhalis
What are the pathogenic (group I) species?
N. gonorrhoeae and N. meningitidis
What are the non-pathogenic species?
N. lacamica, M. catarrhalis, N. cinerea, N. mucosa, N.
sicca, N. subflava, N. flavescens, M. catarrhalis and others
What media will pathogenic species grow on?
Selective
What media will non-pathogenic species grow on?
not selective but some can
Who is GNID on and why not both sexes?
Males it is diagnostic, females cant have it due to normal flora
What does bacteria in joint fluid mean?
significant, should be sterile
What is CSF cultured on?
Chocolate
What are disease associated caused with N. gonorrhoeae?
gonorrhea,eye infections, septicemia, pharyngitis, arthritis
What are disease associated caused with N. meningitides?
maybe normal respiratory flora, meningitis,
septicemia, DIC, Waterhouse Friderichsen syndrome
How is N. gonorrhoeae diagnosis made?
Positive GNID
What is the proper specimen collention and transport for gonorrhea?
Dacron or rayon swabs, urethra in males and endocervix in females
Resistance and treatment trends with N. gonorrhoeae
Widespread, multidrug therapy, also treat co-infection with Chlamydia
What are the advantages and disadvantages of conventional and molecular testing for N. gonorrhoeae?
Advan-rapid, perform on clinical specimen, live organisms not required, chlamydia and tricomanas tested at same time. DISADVAN- not admissible in court, no live organism for susceptibility, expensive
How do most individuals become infected with N. meningitides?
Close contact (young adults) dorms, barracks
What are the distinguishing characteristics of waterhouse-friderichsen syndrome?
hemorrhage into adrenal glands, shock, rapid death
Why is it critical to work under a hood with N, menigitidis and not N. gonorrhoeae?
pilli, Gonorrhoeae loses them and can not infect, Menigitidis can
What is the recommended treatment for at risk N. meningitis people?
Rifampin
Why are those in the US not fully protected against N. meningitids?
type B vaccine just became avaliable
Gray white colonies, hockey puck, rowth on SBA and CHOC, no acid production from carbs, DNase +, butyrase esterase +
Moraxella catarrhalis
What organism is misidentifies as N. gonorrhoeae using carbs?
N. cinerae
Compare and contrast N. cinerae and N. gonorrhoeae
Look similar, N. cinerae is not pathogenic
What are characteristics of less common Neisseria?
Yellow/greenish, dry and wrinkled colonies, N. sicca is G+, M+, L-, S+
