Haemophilus and Bordetella Flashcards
What are the gram characteristics of Haemophilus?
Tiny GNR (can look coccobacillary), can be long filamentous rods
What are the characteristics of Haemophilus?
Very fastidious, Capnophiliic, mousy smell, oxidase AND catalase positive most are opportunistic and adapted the respiratory tract. Require growth factors (i.e. X, V or Both)
What is X factor, where is it found?
Hemin. It is found in chocolate and is HEAT STABLE
What is V factor, where is it found?
NAD, in RBS but inhibited by enzymes in humans. Also present in yeast extract (chocolate agar)
What species require V factor?
All BUT Aggregaatibacter aphrophilus and Haemphilus ducreyi
What are the two methods for identifying X and V factor?
Staph streak or disks
What factors are in T-Soy plates?
None
What factors are in SBA
Only X
What factors are released by stapylcoccus aureus?
V
What factors are in chocolate agar?
X and V
What factors are in Rabbit blood Agar?
X and V
What factors are in Fildes Enrichment agar?
X and V
What characteristic does a rabbit blood plate help to identify? What part of the bacteria name indicates this?
BETA hemolysis, haemolyticus
What is an alternative form of determining X factor? What does it eliminate? What is its basis?
Porphyrin, Eliminates Hemin carryover and it fluroeses
What is the clinical significance of H. flu?
It is a secondary invader with Pneumonia and in noninvasive NTHi (nontypable)