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Microbiology Module 1 '15 - '16 > Catalase Positive GPCs (Parts I and II) > Flashcards

Catalase Positive GPCs (Parts I and II) Flashcards

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Biology 101 flashcards
1
Q

What are the three genera of catalase positive GPCs?

A

Staphylococcus, Micrococcus and Rothia

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2
Q

What are the purposes of Mannitol salt and CNA agars?

A

CNA selects GP and used with normal flora, Salt selects Staphylococcus and turns yellow with S. Aureus used for outbreaks

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3
Q

What are small colony variants?

A

Resistant and relapsing infections (may be resistant to antimicrobials.

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4
Q

What organism turns yellow on mannitol salt?

A

Staphylococcus aureus

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5
Q

What organism is large, opaque, white and usually gamma?

A

Coagulase negative Staph

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6
Q

What organism is large opaque, golden and Beta?

A

Staphylococcus Aureus

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7
Q

What type of plate is a catalase preformed on? Why?

A

Trypticase Soy, Blood gives false pos.

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8
Q

What are two types of coagulase test? Their reagents? how are they preformed?

A

Slide then tube, both use rabbit/human plasma but Slide reacts with clumping and Tube looks for staphylocoagulase

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9
Q

What organism can give a false positive to slide coagulase?

A

S. Lugdunensis

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10
Q

How does S. Lugdunensis react on slide and tube coagulase tests?

A

False pos and Neg respectively

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11
Q

How does lysostaphin work?

A

Lyses Staphylococcus

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12
Q

What test identifies S. Aureus?

A

Tube coagulase is Pos

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13
Q

What test identifies S. Epidermis and S. Saprophyticus

A

Novo susceptible is S. Epidermis.; S. Saprophyticus is Novo Resistant.

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14
Q

What bacteria is Sticky yellow colonies and is a strict aerobe (not facultative)?

A

Micrococcus

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15
Q

How does micrococcus react with glucose in OF glucose test?

A

Oxidizer or non-oxidizer of glucose

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16
Q

What are the patient symptoms and conditions that define toxic shock syndrome?

A

Fever, rash, desquamation, shock, DIC

17
Q

What virulent factor is the main factor for S. Aureus?

A

Protein A

18
Q

What is the function on catalase?

A

Inactivates H2O2

19
Q

What is the the prothrombin like clotting substance?

A

Coagulase

20
Q

MRSA, MSSA, VISA, VRSA

A

Methicillin resistant, Penecillin susceptible, Vancomycin intermediate, Vancomycin resistant

21
Q

Compare and contrast hospital acquired MRSA and community acquired MRSA.

A

Healthcare is highly resistant and requires isolation. Community is not typically as bad and is usually just skin infections of otherwise healthy people.

22
Q

What is the process of screening patients for MRSA?

A

Oxacillin screening plate or PCR

23
Q

What is the precess of screening colonies for MRSA?

A

Cefoxitin disk tests predicts Oxacillin resistance.

24
Q

What is the best way to reduced infections?

A

Handwashing

25
Q

What special result does HACO-MRSA have?

A

requires specialized testing parameters, Increased time and NaCl.

26
Q

What is the term for an infection that looks like a spider bite?

A

Carbuncle

27
Q

What bacteria is associated with dog bites?

A

Coagulates + in the tube, staphylococcus intermedius

28
Q

What are coagulate negative staphylococcus?

A

S, Epidermidis and S. Saprophyticus

29
Q

How do you tell the difference of S. Epidermidis and S. Saprophticus? What do they cause?

A

Saptophyticus is resistant to Novobiocin, and causes urinary tract infections in young females. Epidermidis has biofilm production, is slimy and is the leading cause of endocarditis.

30
Q

How are lugdunensis and aurus confused? How do you differentiate?

A

They are both slide positive, however, only Lugdunensis is tube negative.

31
Q

What bacteria is furazolidone resistant and lysotaphin resistant?

A

Micrococcus

32
Q

What bacteria has beta hemolysis and is yellow?

A

Micrococcus

33
Q

What bacteria causes toxic shock syndrome?

A

Aureus

34
Q

What bacteria causes food poisoning?

A

Aureus

35
Q

What toxin leads to scaled skin syndrome? What bacteria is it from?

A

Exfoliatin; Aureus

36
Q

What catalase positive GPC will be oxidizers or non oxidizers when tested in OF glucose media?

A

Micrococcus

37
Q

What disc prevents oxacillin resistance?

A

Cefoxitin

38
Q

Review chart on GPC for test results to differentiate staph and micrococcus.

A

Review

Microbiology Module 1 '15 - '16 (9 decks)

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