Name that Bug! Flashcards
Lacks muramic acid in its cell wall?
Chlamydia
Lacks sterols in its cell membrane?
Mycoplasma
Stained by Giemsa? (5)
Chlamydia, Rickettsiae, Trypanosomes, Plasmodium, Borrelia
Stained by Ziehl-Neelsen? (2)
Nocardia, Mycobacterium (acid fast!)
Stained by India Ink or mucicarmine?
Cryptococcus
Stained by silver?
Fungi (esp. pneumocystis), Legionella, H. pylori
Cultured on chocolate agar including Factors V/X (NAD/hematin)?
H. flu
Cultured on Thayer-Martin (VPN) media?
Neisseria
Cultured on Bordet-Gengou agar?
Bordetella pertussis
Cultured on Tellurite/Loffler medium?
C. Diphtheriae
Cultured on Lowenstein-Jenson agar?
M. tuberculosis
Cultured on Eaton agar with cholesterol?
Mycoplasma
Pink on MacConkey agar?
Lactose fermentors
- Fast - E. coli, Klebsiella, Enterobacter
- Slow - Citrobacter, Serratia, Others
Metallic sheen on EMB agar?
E. coli
Cultured on charcoal yeast extract agar with cysteine/iron?
Legionella
Cultured on Sabouraud agar?
Fungi
Obligate aerobes (4)
Nagging Pests Must Breathe
- Nocardia
- Pseudomonas
- Mycobacteria
- Bacillus
Obligate anaerobes (3)
Can’t Breathe Air
- Clostradium
- Bacteriodes
- Actinomyces
(lack catalase, SOD; resistant to aminoglycosides due to o2-dependent transport)
Facultative Intracellular (8)
Listen Sally Yer Friend Bruce Must Leave Now
Listeria, Salmonella, Yersinia, Francisella, Brucella, Mycobacteria, Legionella, Neisseria
Encapsulated Bacteria (7) - sickle cell, splenectomy
SHiNE SKiS
S. pneumoniae, H. Flu, N. Meningitis, E. coli, Salmonella, Klebsiella, Strep (Group B)
Catalase-positive organisms (7) - CGD
PLACESS for your “cats”
Pseudomonas, Listeria, Aspergillus, Candida, E. coli, S. aureus, Serratia
Urease-positive organisms (7)
PUNCH & KiSS
Proteus, Ureaplasma, Nocardia, Cryptococcus, H. pylori // Klebsiella, S. epidermidis, S. saprophyticus
Yellow sulfur granules
Actinomyces israeli
Yellow pigment
S. aureus
Blue-green pigment
Pseudomonas aeruginosa
Red pigment
Serratia marcescens
Protein A (Fc region of IgG)
S. aureus
M protein
Group A strep
IgA protease
SHiN - S.pneumo, H.flu, Neisseria
Toxin inactivating EF-2 by ADP-ribosylation –| protein synthesis
C. diphtheriae, P. aeruginosa
Toxin inactivating 60S ribosome by removing adenine from 28S rRNA –| protein synthesis
Shigella, EHEC (O157:H7)
Phage-encoded toxins (6)
ABCDES
- Group A Strep, Bordetella pertussis, Cholera, Diphtheria, EHEC (O157:H7), Shigella
Toxins inhibiting protein synthesis (4)
C. diphtheriae, Pseudomonas, Shigella, EHEC (O157:H7)
Toxins increasing fluid secretion (4)
ETEC (x2), B. anthracis, V. cholerae, B. cereus
Toxins inhibiting phagocytic ability (1)
B. pertussis
Toxins inhibiting release of neurotransmitter (2)
Botulinum, Tetanus
Toxins lysing cell membranes (3)
B. cereus, C. perfringens, S. pyogenes
Toxins that serve as superantigens (2)
S. aureus, S. pyogenes (x2)
Toxins that serve as proteases (5)
Botulinum toxin and tetanus toxin
LF (lethal factor) of Bacillus anthracis cleaves MAPK
Exfoliatin of Staphylococcus aureus cleaves desmoglein
Exotoxin B (SpeB) of certain “flesh-eating” strains of Streptococcus pyogenes
Ability for transformation
SHiN organisms
Transfer of plasmid genes only
F+ x F-
Transfer of plasmid and chromosomal genes
Hfr x F-
Generalized transduction
“Packaging” event, lytic phage
Specialized transduction
“Excision” event, lysogenic phage
Pneumonia after flu
S. aureus
Toxic shock syndrome, scalded skin syndrome, rapid-onset food poisoning
S. aureus
pyrrolidonyl arylamidase- gram+
S. aureus
pyrrolidonyl arylamidase+ gram+
S. pyogenes
2nd MCC of UTI in young woman
S. saprophyticus
1st MCC of UTI in young woman
E. coli
MOPS (meningitis, otitis media, pneumonia, sinusitis)
S. pneumoniae
Lancet-shaped gram+ diplococci
S. pneumoniae
“Rusty sputum” pneumonia, sickle cell sepsis
S. pneumoniae
Binds to fibrin-platelet aggregates on exposed subendothelial GAGs via dextrans
S. viridans (S. sanguinis)
Dental caries
S. viridans (S. mutans)
Pyogenic, toxigenic, and immunologic diseases associated
S. pyogenes
M protein (–| alternative complement)
S. pyogenes
+ ASO titer
S. pyogenes
Scarlet rash with strawberry tongue, sandpaper-like texture, circumoral pallor with desquamation after week #1
S. pyogenes (Scarlet Fever)
Severe pain/swelling –> hypotension/fever (sepsis)
S. pyogenes (Necrotizing fasciitis)
Group A Strep Glomerulonephritis
Impetigo > Pharyngitis
Rheumatic Fever
Pharyngitis (GAS)
Pneumonia, meningitis, and sepsis in a baby
GBS
Produces CAMP factor and enlargens area of hemolysis formed by S. aureus
GBS
Hippurate test +
GBS
Intrapartum penicillin prophylaxis
GBS
Subacute endocarditis following GI/GU procedure
Enterococci
Grow in 6.5% NaCl and bile both
Enterococci
VRE
Enterococci (vancomycin-resistant)
Bacteremia and subacute endocarditis in colon cancer patients
S. bovis
Sore throat, lymphadenopathy, myocarditis, arrhythmias
C. diphtheriae
Metachromatic (blue/red) granules
C. diphtheriae
Elek test +
C. diphtheriae
Throat exudate with fibrin, leuks, bacteria, and necrotic epithelium
C. diphtheriae
“Chinese letter” appearance with V/Y chains
C. diphtheriae
Spastic paralysis, trimus, risus sardonicus
Tetanus
Floppy baby syndrome, flaccid paralysis
Botulinum