MTAP W2 (Hematology W10: Lab Evaluation) Flashcards

1
Q

CBC IS ALSO KNOWN AS

A

HEMOGRAM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

INCLUDES
1. ENUMERATION OF CELLULAR ELEMENTS
2. QUANTITATION OF HGB
3. STATISTICAL ANALYSES OF CELL APPEARANCES

A

CBC/HEMOGRAM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

PREVENTS PLATELETS FROM CLUMPING ON GLASS SLIDE MAKING IT MORE ACCURATE DURING FILM EVALUATION

A

EDTA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

SPECIMEN OF CHOUCE FOR EVALUATION OF BLOOD CELL MORPHOLOGY

A

ANTICOAGULANT-FREE BLOOD

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

PERIPHERAL BLOOD FILM

EDTA BLOOD (PURPLE TOP)
MADE WITHIN – OF DRAWING THE SPECIMEN- HIGH QUALITY FILMS

A

2-3 HOURS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

ADVANTAGES
1. MULTIPLE SLIDES
2. PREP AT LATER TIME
3. AVOIDS PLT CLUMPING

A

EDTA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

PREFERRED EDTA TUBE WHEN MAKING PERIPHERAL BLOOD FILM

IT MIXES MORE EASILY WITH BLOOD

A

TRIPOTASSIUM EDTA/LIQUID FORM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

PERIPHERAL BLOOD FILM

EDTA TUBES THAT REMAIN FOR MORE THAN -

BLOOD CELL ARTIFACTS:
1. VACUOLATED NEUTROPHILS
2. ECHINOCYTIC RBCS
3. NECROBIOTIC LEUKOCYTES
4. SPHEROCYTES

A

5 HOURS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

PERIPHERAL BLOOD FILM

EDTA LEFT FOR 5 HOURS

BLOOD CELL ARTIFACTS (4)

A
  1. VACUOLATED NEUTROPHILS
  2. ECHINOCYTIC RBCS
  3. NECROBIOTIC LEUKOCYTES
  4. SPHEROCYTES

VENS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

PERIPHERAL BLOOD FILM

CAUSE PLATELET SATELLITOSIS

A

EDTA SPECIMEN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

PERIPHERAL BLOOD FILM

PLATELETS SURROUND OR ADHERE TO THE NEUTROPHILS

A

PLATELET SATELLITOSIS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

PERIPHERAL BLOOD FILM

PLATELET SATELLITOSIS:

FALSE DECREASE DUE TO ADHERANCE TO NEUTROPHIL

A

PSEUDOTHROMBOCYTOPENIA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

PERIPHERAL BLOOD FILM

PLATELET SATELLITOSIS:

FALSE INCREASE OF WBC, CLUMPS WILL BE READ AS WBC DUE TO ITS SIZE

A

PSEUDOLEUKOCYTOSIS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

PLATELET SPECIFIC AUTOANTIBODIES THAT REACH BEST AT RT ARE ONE OF THE MECHANISMS KNOWN TO CAUSE THIS PHENOMENON

A

PLATELET SATELLITOSIS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

PERIPHERAL BLOOD FILM

PLATELET SATELLITOSIS:

CORRECTION: RECOLLECT BLOOD SAMPLE USING -

A

3.2% NA CITRATE (BLUE TOP)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

PERIPHERAL BLOOD FILM

PLATELET SATELLITOSIS:

CORRECTION: RECOLLECT BLOOD SAMPLE USING 3.2 NA CITRATE

ADJUSTMENT MUST MULTIPLY TO -

A

PLATELET COUNT X 1.1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

PERIPHERAL BLOOD FILM

ADVANTAGE:
1. MADE AT PX SIDE
2. SOME ARTIFACTS MAY BE PREVENTED

A

ANTICOAGULANT-FREE BLOOD

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

METHODS OF BLOOD FILM PREPARATION

EASIEST, MOST CONVENIENT, COMMONLY USED

A

MANUAL/PUSH-WEDGE METHOD

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

METHODS OF BLOOD FILM PREPARATION

MANUAL WEDGE TECHNIQUE aka PUSH-WEDGE METHOD

USES SLIDE AS PUSHER/SPREADER
FILM SLIDE: 3X1 INCH (75 X25 MM)
ANGLE OF PUSHER/SLIDE: -

A

30-45 DEGREE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

METHODS OF BLOOD FILM PREPARATION

MANUAL WEDGE TECHNIQUE aka PUSH-WEDGE METHOD

BLOOD IS DROPPED AT ABOUT - FROM END OR FROSTED

A

0.25/ 1/4 INCH
1 CM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

METHODS OF BLOOD FILM PREPARATION

MANUAL WEDGE TECHNIQUE aka PUSH-WEDGE METHOD

DROP OF BLOOD: -

A

2-3MM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

METHODS OF BLOOD FILM PREPARATION

MANUAL WEDGE TECHNIQUE aka PUSH-WEDGE METHOD

DROP OF BLOOD: 2-3MM
LARGE AMOUNT OF BLOOD= - SMEAR

A

THICKER/LONGER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

METHODS OF BLOOD FILM PREPARATION

MANUAL WEDGE TECHNIQUE aka PUSH-WEDGE METHOD

DROP OF BLOOD: 2-3MM
SMALL AMOUNT OF BLOOD= -SMEAR

A

THINNER/SHORT

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

METHODS OF BLOOD FILM PREPARATION

MANUAL WEDGE TECHNIQUE aka PUSH-WEDGE METHOD

SPEED OF SPREADER:
TOO FAST= - SMEAR

A

THICKER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

METHODS OF BLOOD FILM PREPARATION

MANUAL WEDGE TECHNIQUE aka PUSH-WEDGE METHOD

SPEED OF SPREADER:
TOO SLOW= - SMEAR

A

THINNER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

METHODS OF BLOOD FILM PREPARATION

MANUAL WEDGE TECHNIQUE aka PUSH-WEDGE METHOD

HEMATOCRIT OF PX:
TOO HIGH (>60%) ANGLE SHOULD BE LOWERED AS LOW AS -

A

25 DEGREE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

METHODS OF BLOOD FILM PREPARATION

MANUAL WEDGE TECHNIQUE aka PUSH-WEDGE METHOD

HEMATOCRIT OF PX:
TOO LOW (>45%) ANGLE SHOULD BE RAISED -

A

> 45 DEGREES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

METHODS OF BLOOD FILM PREPARATION

TOO THICK SMEARS
PRESSURE: -

A

LOW

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

METHODS OF BLOOD FILM PREPARATION

TOO THICK SMEARS
ANGLE: -

A

HIGH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

METHODS OF BLOOD FILM PREPARATION

TOO THICK SMEARS
SPEED: -

A

FAST

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

METHODS OF BLOOD FILM PREPARATION

TOO THICK SMEARS
SIZE OF BLOOD: -

A

LARGE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

PERIPHERAL BLOOD FILM

COVERSLIP METHOD OF BLOOD FILM PREPARATION

  • METHOD: GLASS SLIDE + COVERSLIP
A

BEACOM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

PERIPHERAL BLOOD FILM

COVERSLIP METHOD OF BLOOD FILM PREPARATION

  • METHOD: 2 COVER SLIP METHOD
A

EHRLICH METHOD

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

PERIPHERAL BLOOD FILM

FEATURES OF A WELL-MADE WEDGE PBS

FILM IS - LENGTH OF SLIDE

A

2/3-3/4

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

PERIPHERAL BLOOD FILM

FEATURES OF A WELL-MADE WEDGE PBS

THE FILM SHOULD BE - SHAPED NOT BULLET

A

FINGER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

PERIPHERAL BLOOD FILM

FEATURES OF A WELL-MADE WEDGE PBS

SLIDE HELD UP TO THE LIGHT THIN PORTION MUST BE HAVE A - APPEARANCE

A

RAINBOW

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

PERIPHERAL BLOOD FILM

FILM THAT IS - (COLOR) THAN NORMAL MAY INDICATE THAT THE PX HAS

  1. INCREASED BLOOD PROTEINS
  2. PLASMA CELL MYELOMA
  3. ROULEAUX
A

BLUER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

PERIPHERAL BLOOD FILM

A - APPEARANCE TO THE FILM MAY INDICATE RBC AGGLUTINATION FOUND IN COLD HEMAGGLUTIN DISEASES

A

GRAINY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

PERIPHERAL BLOOD FILM

  • ALL OVER THE FILM INDICATES INCREASED LIPID LEVELS
A

HOLES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
40
Q

PERIPHERAL BLOOD FILM

SLIDES STAINED AFTER - OR LONGER TURN OUT TOO BLUE

SHOULD ONLY BE STORED FOR -

A

1 WEEK

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
41
Q

PERIPHERAL BLOOD FILM

FILMS PRODUCED EVERY 30SEC

A

SYSMEX SP10

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
42
Q

PERIPHERAL BLOOD FILM

AUTOMATED SLIDE STAINERS

A

MIDAS AND HEMA TEK

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
43
Q

PERIPHERAL BLOOD FILM

DEMONSTRATES LE CELLS
CONCENTRATES THE NUCLEATED CELLS PRESENT

A

BUFFY COAT SMEAR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
44
Q

PERIPHERAL BLOOD FILM

USES BUFFY COAT SMEAR ON PATIENTS WITH EBC COUNT

A

<1X10^9/L

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
45
Q

PERIPHERAL BLOOD FILM

-BLOOD SMEAR USED FOR SCREENING OF BLOOD PARASIRES

A

THICK SMEARS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
46
Q

PERIPHERAL BLOOD FILM

  • STAIN USED FOR BM AND PBS
A

WRIGHT/ GIEMSA STAIN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
47
Q

PERIPHERAL BLOOD FILM

FIXATIVE: -

A

METHANOL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
48
Q

PERIPHERAL BLOOD FILM

ACTUAL STAINING DOES NOT OCCUR UNTIL - IS ADDED

A

BUFFER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
49
Q

PERIPHERAL BLOOD FILM

-: STAINS ACIDIC PARTS OF CELL SUCH AS RNA

A

METHYLENE BLUE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
50
Q

PERIPHERAL BLOOD FILM

-: STAINS BASIC PART OF CELL

A

EOSIN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
51
Q

PERIPHERAL BLOOD FILM

OXIDIZE METHYLENE BLUE AND EOSIN
STAINS NEUTRAL COMPONENT

A

THIZAINE-EOSINATE COMPLEX

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
52
Q

PERIPHERAL BLOOD FILM

BUFFER IS ADDED TO STAIN SHOULD BE -M

A

0.05M SODIUM PHOSPHATE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
53
Q

PERIPHERAL BLOOD FILM

BUFFER ADDED TO STAIN FOR ATLEAST 24 HOURS

A

AGED DISTILLED WATER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
54
Q

MOST IMPORTANT PART OF STAINING

A

PH 6.4-6.8

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
55
Q

PERIPHERAL BLOOD FILM

PH FOR MALARIAL PARASITES

A

7.2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
56
Q

WELL STAINED PERIPHERAL BLOOD FILM

MACRO: SHOULD BE (COLOR)

A

PINK TO PURPLE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
57
Q

WELL STAINED PERIPHERAL BLOOD FILM

MICRO: RBC SHOULD APPEAR

A

ORANGE-SALMON PINK

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
58
Q

WELL STAINED PERIPHERAL BLOOD FILM

MICRO: WBC SHOULD BE

A

PURPLE TO BLUE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
59
Q

WELL STAINED PERIPHERAL BLOOD FILM

MICRO: CYTOPLASM OF NEUTROPHILS SHOULD BE

A

PINK TO TAN WITH VIOLET GRANULES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
60
Q

WELL STAINED PERIPHERAL BLOOD FILM

MICRO: EOSINOPHILS SHOULD HAVE - GRANULES

A

BRIGHT ORANGE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
61
Q

WELL STAINED PERIPHERAL BLOOD FILM

EXCESSIVELY - (COLOR) STAIN

  1. THICK
  2. PROLONGED STAINING
  3. INADEQUATE WASHING
  4. TOO ALKALINE
A

BLUE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
62
Q

PERIPHERAL BLOOD FILM

STAINING PROBLEM:
FAILURE TO HOLD THE SLIDE -

A

HORIZONTALLY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
63
Q

COMPLETE BLOOD COUNT

PERFORMED USING A - OR -

A

HEMACYTOMETER/ COUNTING CHAMBER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
64
Q

MOST COMMONLY USED HEMACYTOMETER

A

LEVY WITH NEUBAUER RULING

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
65
Q

LEVY WITH NEUBAUER RULING

COMPOSED OF -MMX-MM SQUARE COUNTING AREA SEPARATED BY AN H SHAPED MOAT

A

3X3MM
TOTAL= 9MM2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
66
Q

LEVY WITH NEUBAUER RULING

TOTAL VOLUMR OF ONE ENTIRE GRID OF HEMACYTOMETER IS

A

0.9MM3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
67
Q

LEVY WITH NEUBAUER RULING

  • EACH SECONDARY SQUARE EXCEPT CENTER
A

16 SQUARES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
68
Q

LEVY WITH NEUBAUER RULING

CENTER SECONDARY SQUARES -

A

25 SQUARES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
69
Q

LEVY WITH NEUBAUER RULING

READING OF PLATELETS

A

CENTER SQUARE
25 SECONDARY SQUARES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
70
Q

LEVY WITH NEUBAUER RULING

READING OF RBC

A

CENTER SQUARE
ONLY 4 CORNER AND CENTER OF SECONDARY SQUARES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
71
Q

LEVY WITH NEUBAUER RULING

READING OF WBC

A

4 CORNER SQUARES
16 SECONDARY SQUARES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
72
Q

LEVY WITH NEUBAUER RULING

CORNER SQUARES
16 SECONDARY SQUARES EACH WITH AREA OF -

A

0.0625 MM2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
73
Q

LEVY WITH NEUBAUER RULING

CENTER SQUARE
25 SECONDARY SQUARES EACH WITH AREA OF -

A

0.04MM2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
74
Q

HEMACYTOMETER

DEPTH: -

A

0.1MM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
75
Q

HEMACYTOMETER

DEPTH FACTOR: -

A

1/10 OR 10

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
76
Q

HEMACYTOMETER

DISTANCE BETWEEN THE BOTTOM OF THE COVERSLIP AND SURFACE OF COUNTING AREA

A

DEPTH FACTOR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
77
Q

DILUTING PIPETTES

COMPOSED OF STEM DIVIDED INTO - EQUAL PARTS WITH BEAD THAT AIDS IN MIXING THE DILUENT

A

10 EQUAL PARTS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
78
Q

DILUTING PIPETTES

IN CALCULATING DILUTION, ONLY THE VOLUME CONTAINED IN THE - IS CONSIDERED

A

BULB

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
79
Q

DILUTING PIPETTES

RBC PIPETTE: - MARK/ TOTAL UNITS

A

101 MARK

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
80
Q

DILUTING PIPETTES

WBC PIPETTE: - MARK/ TOTAL UNITS

A

11 MARK

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
81
Q

DILUTING PIPETTES

RBC PIPETTE

BULB: - UNIT

A

100 UNIT

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
82
Q

DILUTING PIPETTES

RBC PIPETTE

STEM: - UNIT

A

1 UNIT

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
83
Q

DILUTING PIPETTES

RBC PIPETTE
BLOOD DRAWN ON 0.5 MARK
DILUTION: -

A

1:200

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
84
Q

DILUTING PIPETTES

RBC PIPETTE
BLOOD DRAWN ON 1.0 MARK
DILUTION: -

A

1:100

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
85
Q

DILUTING PIPETTES

RBC PIPETTE
DILUENT IS DRAWN TO THE - MARK

A

101 MARK

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
86
Q

DILUTING PIPETTES

WBC PIPETTE
BLOOD DRAWN ON 0.5 MARK
DILUTION: -

A

1:20

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
87
Q

DILUTING PIPETTES

WBC PIPETTE
BLOOD DRAWN ON 1.0MARK
DILUTION: -

A

1:10

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
88
Q

DILUTING PIPETTES

WBC PIPETTE
DILUENT IS DRAWN TO THE - MARK

A

11 MARK

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
89
Q

DILUTING PIPETTES

WBC PIPETTE
VOLUME OF THE BULB

A

10

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
90
Q

GENERAL FORMULA FOR MANUAL CELL COUNTS

A

TC= CC X DF/ AREA X 0.1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
91
Q

GENERAL FORMULA FOR MANUAL CELL COUNTS

THE DIFFERENCE BETWEEN THE TOTAL CELLS COUNTED ON EACH SIDE SHOULD BE -

A

<10%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
92
Q

MANUAL CELL COUNT

CONVENTIONAL UNIT

A

CELLS/UL
CELLS/MM3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
93
Q

MANUAL CELL COUNT

SI UNIT

A

CELLS/L

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
94
Q

MANUAL CELL COUNT

REPORTING RBC
CONVENTIONAL UNIT

A

X10^6/MM3
X10^6/UL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
95
Q

MANUAL CELL COUNT

REPORTING WBC/PLPATELETS
CONVENTIONAL UNIT

A

X10^3/MM3
X10^3/UL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
96
Q

MANUAL CELL COUNT

REPORTING RBC
SI UNIT

A

X10^12/L

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
97
Q

MANUAL CELL COUNT

REPORTING WBC/PLATELETS
SI UNIT

A

X10^9/L

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
98
Q

WBC
DILUTION: 1:20
OBJECTIVE: 10X (LPO)
AREA COUNTED: -

A

4MM2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
99
Q

WBC
DILUTION: 1:100
OBJECTIVE:10X (LPO)
AREA COUNTED: -

A

9MM2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
100
Q

RBC
DILUTION: 1:100
OBJECTIVE: 40X (HPO)
AREA COUNTED: -

A

0.2MM2/ 5 SMALL SQAURES OF CENTER SQUARE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
101
Q

PLATELETS
DILUTION: 1:100
OBJECTIVE: 40X PHASE CONTRAST
AREA COUNTED: -

A

1MM2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
102
Q

WBC DILUTING FLUIDS

A
  1. 1% AMMONIUM OXALATE
  2. 1% HYDROCHLORIC ACID
  3. 3% ACETIC ACID
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
103
Q

RBC DILUTING FLUID

A

ISOTONIC SALINE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
104
Q

PLATELETS DILUTING FLUID

A

1% AMMONIUM OXALATE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
105
Q

MANUAL RBC COUNT ARE RARELY PERFORMED

(2) CONCENTRATIONS ARE MORE DESIRABLE WHEN AUTOMATION IS NOT AVAILABLE

A
  1. MICROHEMATOCRIT
  2. HEMOGLOBIN
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
106
Q

NORMAL RBC COUNT
MALE: -/L

A

4.20-6.00
X10^12/L

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
107
Q

NORMAL RBC COUNT
FEMALE: -/L

A

3.80-5.20
X10^12/L

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
108
Q

MANUAL RBC COUNT

AREA OF COUNTING CHAMBER USED: 5 CENTER SQUARES

EACH SQUARE: -

A

0.04MM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
109
Q

MANUAL RBC COUNT

AREA OF COUNTING CHAMBER USED: 5 CENTER SQUARES

5 SQUARES: -

A

0.2MM2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
110
Q

MANUAL RBC COUNT

MICROSCOPE OBJECTIVE: -

A

40X/ HPO

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
111
Q

MANUAL RBC COUNT

USUAL DILUTION: -

A

1:100

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
112
Q

MANUAL RBC COUNT

RBC DILUTING FLUIDS SHOULD BE - TO FACILITATE COUNTING AND PREVENT LYSIS OF RBCS

A

ISOTONIC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
113
Q

MANUAL RBC COUNT

BEST DILUTING FLUID FOR RBC

A

DACIES/FORMOL CITRATE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
114
Q

MANUAL RBC COUNT

RBC DILUTING FLUIDS (7)

A

1.DACIES/FORMOL CITRATE
2.3.8% SODIUM CITRATE
3.NSS
4.BETTHELL’S
5.HAYEM’S
6.TOISSON’S
7.GOWER’S

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
115
Q

RBC PARAMETERS REPORTING

RBC COUNT

A

RBCS
X10^12/L

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
116
Q

RBC PARAMETERS REPORTING

HGB

A

g/dL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
117
Q

RBC PARAMETERS REPORTING

HCT

A

% or L/L

118
Q

RBC PARAMETERS REPORTING

MCV

A

fL

119
Q

RBC PARAMETERS REPORTING

MCH

A

pg

120
Q

RBC PARAMETERS REPORTING

MCHC

A

g/dL

121
Q

RBC PARAMETERS REPORTING

RDW

A

%

122
Q

AN IMPORTANT TOOL TO ASSESS BM’S ABILITY TO INCREASE RBC PRODUCTION IN RESPONSE TO ANEMIA

A

RETICULOCYTE COUNT

123
Q

ERYTHROCYTE MATURATION SERIES

LAST STAGE CAPABLE OF HGB SYNTHESIS

A

RETICULOCYTE

124
Q

YOUNG RBC THAT LACKS NUCLEUS BUT CONTAINS RESIDUAL RNA TO COMPLETE PRODUCTION OF HGB

A

RETICULOCYTE

125
Q

RETICULOCYTE

SPENDS - IN THE BM

A

2 DAYS

126
Q

RETICULOCYTE

SPENDS - IN THE PERIPHERAL BLOOD BEFORE DEVELOPING INTO A MATURE RBC

A

1 DAY

127
Q

RETICULOCYTE COUNT

ADULT: -

A

0.5-1.5%

128
Q

RETICULOCYTE COUNT

NEWBORN: -

A

2-6%

129
Q

RETICULOCYTE

STAINED WITH -

A

SUPRAVITAL STAIN- NEW METHYLENE BLUE

130
Q

RETICULOCYTE COUNT

RATIO OF BLOOD AND STAIN

A

1:1

131
Q

RETICULOCYTE COUNT

INCUBATE AT RT FOR -

A

3-10MIN

132
Q

RETICULOCYTE COUNT

COUNT - RBCS UNDER - LENS

A

1000, OIO

133
Q

RETICULOCYTE COUNT

FOR ACCURACY, HAVE ANOTHER LABORATORIAN TO COUNT SHOULD AGREE WITHIN -

A

20%

134
Q

RETICULOCYTE COUNT

FORMULA

A

RETIC%= NO.OF RETIC X 100/ 1000 (RBCS COUNTED)

135
Q

THE ACTUAL NUMBER OF RETICULOCYTES IN 1 LITER OR 1 MICROLITER OF BLOOD

A

ABSOLUTE RETICULOCYTE COUNT

136
Q

ABSOLUTE RETICULOCYTE COUNT

REFERENCE INTERVAL

A

20X109/L
115X109/L

137
Q

ABSOLUTE RETICULOCYTE COUNT

FORMULA

A

ARC= RETICS X RBC COUNT/100

138
Q

IN SPX WITH LOW HEMATOCRIT, RETICULOCYTES MAY BE FALSELY -

A

ELEVATED

139
Q

CORRECTED RETICULOCYTE COUNT

AVERAGE NORMAL HCT -%

A

45%

140
Q

CORRECTED RETICULOCYTE COUNT

FORMULA

A

CRC= RETIC X (PX HCT/45)

141
Q

CORRECTED RETICULOCYTE COUNT

PX WITH HEMATOCRIT OF 35% SHOULD HAVE AN ALEVATED RETICULOCYTE COUNT OF - TO -%

35%=MILD ANEMIA

A

2-3%

142
Q

CORRECTED RETICULOCYTE COUNT

PX WITH HEMATOCRIT OF LESS THAN 25% COUNT SHOULD INCREASE TO - TO -%

<25%= MODERATE ANEMIA

A

3-5%

143
Q

GENERAL INDICATOR OF THE RATE OF ERYTHROCYTE PRODUCTION INCREASE ABOVE NORMAL IN ANEMIAS

A

RETICULOCYTE PRODUCTION INDEX

144
Q

RETICS THAT ARE RELEASE FROM BM PREMATURELY ARE CALLED -

USUALLY TO COMPENSATE ANEMIA

A

SHIFT RETICULOCYTES

145
Q

SHIFT RETICS TAKES - TO - DAYS TO LOSE THEIR RETICULA

A

2-3 DAYS

146
Q

RETICULOCYTE PRODUCTION INDEX

FORMULA

A

RPI= CORRECTED RETIC COUNT/MATURATION TIME

147
Q

THE PX - IS USED TO DETERMINE APPROPRIATE CORRECTION FACTOR

A

HEMATOCRIT

148
Q

RETICULOCYTE PRODUCTION INDEX

PX HEMATOCRIT VALUE %
40-45

CORRECTION FACTOR/MATURATION TIME/DAYS

A

1

149
Q

RETICULOCYTE PRODUCTION INDEX

PX HEMATOCRIT VALUE %
35-39

CORRECTION FACTOR/MATURATION TIME/DAYS

A

1.5

150
Q

RETICULOCYTE PRODUCTION INDEX

PX HEMATOCRIT VALUE %
25-34

CORRECTION FACTOR/MATURATION TIME/DAYS

A

2

151
Q

RETICULOCYTE PRODUCTION INDEX

PX HEMATOCRIT VALUE %
15-24

CORRECTION FACTOR/MATURATION TIME/DAYS

A

2.5

152
Q

RETICULOCYTE PRODUCTION INDEX

PX HEMATOCRIT VALUE %
<15

CORRECTION FACTOR/MATURATION TIME/DAYS

A

3

153
Q

RETICULOCYTE PRODUCTION INDEX

ADEQUATE BM RESPONSE INDICATED BY AN RPI -

A

> 3

154
Q

RETICULOCYTE PRODUCTION INDEX

INADEQUATE ERYTHROPOIETIC RESPONSE IS SEEN WHEN THE RPI -

A

<2

155
Q

RETICULOCYTE COUNT

DESIGNED TO REDUCE LABOR INTESIVE PROCESS

DISC INSERTED INTO THE EYEPIECE OF MICROSCOPE

A

MILLER DISK

156
Q

RETICULOCYTE COUNT

2 SQUARES SMALLER SQUARE 1/8

A

MILLER DISK

157
Q

RETICULOCYTE COUNT
MILLER DISK

  • SQUARE ISWHERE RETICS ARE COUNTED
A

LARGE SQUARE

158
Q

RETICULOCYTE COUNT
MILLER DISK

  • SQUARE IS WHERE RBCS ARE COUNTED
A

SMALL SQUARE 1/9

159
Q

RETICULOCYTE COUNT
MILLER DISK

RBCS

MINIMUM OF - CELLS SHOULD BE COUNTED IN SMALL SQUARE

A

112 CELLS

160
Q

RETICULOCYTE COUNT
MILLER DISK

RBCS

MINIMUM OF 112 CELLS IS EQUIVALENT TO - RED CELLS IN LARGE SQUARE

A

1008

161
Q

RETICULOCYTE COUNT
MILLER DISK

FORMULA

A

RETICS= NO. OF RETICCS IN SQUARE A OR LARGE SQUARE X 100/ NO. OF RBCS IN SQUARE B OR SMALL SQUARE X 9

162
Q
  1. BLOOD LOSS
  2. HEMOLYTIC ANEMIA
  3. TREATMENT TO PERNICIOUS ANEMIA, FOLIC ACID DEFICIENCY OR IRON DEFICIENCY
A

INCREASED RETICULOCYTES

163
Q

MANUAL WBC COUNT

NORMAL WBC COUNT

A

4.0-11.0
X10^9/L

164
Q

MANUAL WBC COUNT

USUAL DILUTION

A

1:20

165
Q

MANUAL WBC COUNT

WBC DILUTING FLUIDS SHOULD BE - TO SWELL AND LYSE RBCS

A

HYPOTONIC

166
Q

MANUAL WBC COUNT

WBC DILUTING FLUIDS (4)

A
  1. 1% AMMONIUM OXALATE
  2. 1% HYDROCHLORIC ACID
  3. 2-3% ACETIC ACID
  4. TURKS DILUTING FLUID
167
Q

MANUAL WBC COUNT

ANTICIPATED WBC: 0.1-0.3
RECOMMENDED DILUTION:-
TYPE OF PIPETTE: -

A

1:10, WBC

168
Q

MANUAL WBC COUNT

ANTICIPATED WBC: 3.1-30.0
RECOMMENDED DILUTION:-
TYPE OF PIPETTE: -

A

1:20, WBC

169
Q

MANUAL WBC COUNT

ANTICIPATED WBC: >30.0
RECOMMENDED DILUTION:-
TYPE OF PIPETTE: -

A

1:100, RBC

170
Q

MANUAL WBC COUNT

ANTICIPATED WBC: >100.0
RECOMMENDED DILUTION:-
TYPE OF PIPETTE: -

A

1:200, RBC

171
Q

MANUAL WBC COUNT

MAKE A 1:20 DILUTION BY PLACING - UL OF BLOOD IN 475 UL OF WBC DILUTING FLUID

A

25UL

172
Q

MANUAL WBC COUNT

MAKE A 1:20 DILUTION BY PLACING 25 UL OF BLOOD IN -UL OF WBC DILUTING FLUID

A

475UL

173
Q

MANUAL WBC COUNT

ALLOW DILUTION TO SIT FOR - TO ENSURE RBC HAVE LYSED

SOLUTION WILL TURN (-COLOR)

A

10MINS, CLEAR

174
Q

MANUAL WBC COUNT

CHARGE SIDES OF HEMACYTOMETER AT A -DEGREE ANGLE

A

45

175
Q

MANUAL WBC COUNT

AFTER CHARGING, PLACE IN CHAMBER FOR - TO SETTLE

A

10MINS

176
Q

THE - ARE COUNTED AS WBCS BC THEY ARE INDISTINGUISHABLE WHEN SEEN IN HEMACYTOMETER

A

NRBCS

177
Q

WBC SHOULD BE CORRECTED WHEN NRBCS ARE

ADULT: - NRBC PER 100 WBC

A

> 5

178
Q

WBC SHOULD BE CORRECTED WHEN NRBCS ARE

NEWBORN: - NRBC PER 100 WBC

A

> 10

179
Q

CORRECTED WBC COUNT

FORMULA

A

UWBCC X 100/ NRBCS + 100

180
Q

WBC DIFFERENTIAL COUNT

FOR ROUTINE TESTING, - SMEAR IS THE MOST WIDELY USED

A

WEDGE SMEAR

181
Q

WBC DIFFERENTIAL COUNT

SCANNING / COUNTING METHODS

WBCS ARE COUNTED IN CONSECUTIVE FIELDS AS THE BLOOD FILM IS MOBED FROM SIDE TO SIDE

A

CROSS SECTIONAL/ CRENELLATION

182
Q

WBC DIFFERENTIAL COUNT

SCANNING / COUNTING METHODS

WBCS ARE COUNTED IN CONSECUTIVE FIELDS FROM TAIL TOWARD THE HEAD OF THE SMEAR

A

LONGITUDINAL

183
Q

WBC DIFFERENTIAL COUNT

SCANNING / COUNTING METHODS

BEGGINING NEAR THE TAIL ON A HORIZONTAL EDGE

A

BATTLEMENT/TRACK/BACK AND FORTH SERPENTINE

184
Q

WBC DIFFERENTIAL COUNT

SCANNING / COUNTING METHODS

MOST PREFERRED COUNTING METHOD

A

BATTLEMENT/TRACK/BACK AND FORTH SERPENTINE

185
Q

WBC DIFFERENTIAL COUNT

  • DIFFERENTIAL COUNT

IF PX WBC COUNT IS <1.0X10^9/L
MULTIPLY RESULTS BY 2

A

50

186
Q

WBC DIFFERENTIAL COUNT

  • DIFFERENTIAL COUNT

ROUTINELY USED

A

100

187
Q

WBC DIFFERENTIAL COUNT

  • DIFFERENTIAL COUNT

OVER 10% EOSINOPHILS
OVER 2% BASOPHILS
OVER 11% MONOCYTES
MORE LYMPO THAN NEUTRO EXCEPT IN CHILDREN

A

200

188
Q

WBC DIFFERENTIAL COUNT

  • DIFFERENTIAL COUNT

WHEN WBC COUNT IS HIGHER THAN 40X10^9/L

A

200

189
Q

WBC DIFFERENTIAL COUNT

  • DIFFERENTIAL COUNT

WHEN WBC COUNT IS >100X10^9/L

A

300-400

190
Q

WBC DIFFERENTIAL COUNT

NEUTROPHILS
RELATIVE COUNT: -

A

50-70
1.7-7.5

191
Q

WBC DIFFERENTIAL COUNT

LYMPHOCYTES
RELATIVE COUNT: -

A

18-42%
1.0-3.2

192
Q

WBC DIFFERENTIAL COUNT

MONOCYTES
RELATIVE COUNT: -

A

2-11%
0.1-1.3

193
Q

WBC DIFFERENTIAL COUNT

EOSINOPHILS
RELATIVE COUNT: -

A

1-3%
0-0.3

194
Q

WBC DIFFERENTIAL COUNT

BASOPHILS
RELATIVE COUNT: -

A

0-2%
0-0.2

195
Q

WBC DIFFERENTIAL COUNT

TOTAL WHITE CELL COUNT ESTIMATION

4000-7000 WBC/UL

A

2-5 WBC/HPF

196
Q

WBC DIFFERENTIAL COUNT

TOTAL WHITE CELL COUNT ESTIMATION

7000-10000 WBC/UL

A

4-6 WBC/HPF

197
Q

WBC DIFFERENTIAL COUNT

TOTAL WHITE CELL COUNT ESTIMATION

10000-13000 WBC/UL

A

6-10 WBC/HPF

198
Q

WBC DIFFERENTIAL COUNT

TOTAL WHITE CELL COUNT ESTIMATION

13000-18000 WBC/UL

A

10-20 WBC/HPF

199
Q

WBC DIFFERENTIAL COUNT

TOTAL WHITE CELL COUNT ESTIMATION

WBC PER FIELD X - IF USING HPO

A

2,000

200
Q

WBC DIFFERENTIAL COUNT

TOTAL WHITE CELL COUNT ESTIMATION

WBC PER FIELD X - IF USING OIO

A

3,000

201
Q

WBC DIFFERENTIAL COUNT

PRESENCE OF IMMATURE GRANULOCYTIC CELLS

LEUKEMIA, BACTERIAL INFX

SHIFT TO THE -

A

LEFT

202
Q

LIST OF SOME CONDITIONS ASSOCIATED WITH SPECIFIC INCREASE OF WBC

-APPENDICITIS
-BACTERIAL INFX
-PANCREATITIS

A

NEUTROPHILIA

203
Q

LIST OF SOME CONDITIONS ASSOCIATED WITH SPECIFIC INCREASE OF WBC

-VIRAL INFX
-INFECTIOUS MONONUCLEOSIS

A

LYMPHOCYTOSIS

204
Q

LIST OF SOME CONDITIONS ASSOCIATED WITH SPECIFIC INCREASE OF WBC

-THYPOID
-RICKETSSIAL INFX
-GAUCHERS

A

MONOCYTOSIS

205
Q

LIST OF SOME CONDITIONS ASSOCIATED WITH SPECIFIC INCREASE OF WBC

-ALLERGIES
-PARASITIC INFX

A

EOSINOPHILIA

206
Q

LIST OF SOME CONDITIONS ASSOCIATED WITH SPECIFIC INCREASE OF WBC

-ALLERGIC REACTION
-TYPE 1 HYPERSENSITIVITY

A

BASOPHILIA

207
Q

HEMOGLOBIN DETERMINATION

THE - METHOD FOR HGB DETERMINATION IS THE REFERENCE METHOD

A

CYANMETHEMOGLOBIN

208
Q

TEST USED TO DIAGNOSE AND FOLLOW TREATMENT OF ANEMIA

VARIES WITH
1. AGE
2. SEX

A

HGB DETERMINATION

209
Q

HEMOGLOBIN DETERMINATION

NORMAL VALUES:
WOMEN: -

A

12-15 g/dL

210
Q

HEMOGLOBIN DETERMINATION

NORMAL VALUES:
MEN: -

A

13.5-18 g/dL

211
Q

HGB IS HIGHER IN THE - AND LOWER IN THE -

A

HIGH- MORNING
LOW-EVENING

212
Q

HEMOGLOBIN DETERMINATION

  • HGB
    STRENOUS MUSCULAR ACTIVITY
    SMOKER
    HIGH ALTITUDE
A

INCREASED

213
Q

HEMOGLOBIN DETERMINATION

  • HGB
    ANEMIA
    AFTER 50 YRS OF AGE
    LYING DOWN
    DEHYDRATION
A

DECREASED

214
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
USES HCL

A

ACID HEMATIN, DIRECT VISUAL

215
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
USES NaOH

A

ALKALI HEMATIN, DIRECT VISUAL

216
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
ACID HEMATIN

HGB TURNS TO - HEMATIN

A

BROWN COLORED

217
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
ACID HEMATIN

ACID HEMATIIN + DW MATCH COLOR

A

YELLOW-BROWN

218
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
MOST RELIABLE AND RECOMMENDED METHOD FOR HGB

A

CYANMETHEMOGLOBIN, INDIRECT/PHOTOELECTRIC

219
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
CYANMETHEMOGLOBIN USES - REAGENT

A

DRABKIN

220
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
CYANMETHEMOGLOBIN

COLOR INTENSITY: -

A

540 NM

221
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
CYANMETHEMOGLOBIN

ALL CAN BE MEASURED EXCEPT FOR - HEMOGLOBIN

A

SULFHEMOGLOBIN

222
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
CYANMETHEMOGLOBIN

OVERANTICOAGULATION - RESULTS

A

DOES NOT EFFECT

223
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
CYANMETHEMOGLOBIN

TURBIDITY WILL CAUSE - RESULTS

A

FALSELY ELEVATED

224
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
CYANMETHEMOGLOBIN

converts Hemoglobin (w/ ferrous iron) to methemoglobin

A

POTASSIUM FERRICYANIDE

225
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
CYANMETHEMOGLOBIN

converts methemoglobin to cyanmethemoglobin

A

POTASSIUM CYANIDE

226
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
CYANMETHEMOGLOBIN

improves lysis of RBC and decrease turbidity

A

NON IONIC DETERGENT

227
Q

HEMOGLOBIN DETERMINATION

COLORIMETRIC METHODS:
CYANMETHEMOGLOBIN

allows the solution to be read after 3 minutes

A

DIHYDROGEN POTASSIUM PHOSPHATE

228
Q

HEMOGLOBIN DETERMINATION

SPECTROPHOTOMETER: 540NM
SET -% TRANSMITTANCE

A

100%

229
Q

HEMOGLOBIN DETERMINATION

DILUTION

A

1:251

230
Q

HEMOGLOBIN DETERMINATION

STANDARD HGB CURVE
HEMOGLOBIN VALUE (G/DL)

A

X-AXIS
ABSCISSA

231
Q

HEMOGLOBIN DETERMINATION

STANDARD HGB CURVE
OPTICAL DENSITY (ABSORBANCE)

A

Y-AXIS
ORDINATE

232
Q

HEMOGLOBIN DETERMINATION

TURBIDITY=FALSELY ELEVATED DUE TO

A

HIGHER ABSORBANCE

233
Q

HEMOGLOBIN DETERMINATION

REMEDY:
Centrifuge then read the absorbance of supernatant

A

High WBC count (>20x109
/L)
High platelet count (>700 x109
/L)

234
Q

HEMOGLOBIN DETERMINATION

REMEDY:
make a 1:1 dilution with distilled water then multiply result by 2

A

Presence of Hb S or Hb C

235
Q

HEMOGLOBIN DETERMINATION

REMEDY:
Use patient blank (0.01ml patient plasma + 5ml Drabkin’s reagent)

A

Lipemic sample

236
Q

HEMOGLOBIN DETERMINATION

REMEDY:
Increase alkalinity of the hemiglobincyanide reagent by
adding potassium carbonate then repeat determination.

A

Abnormal globulins

237
Q

HEMOGLOBIN DETERMINATION

If the drop sinks, its SG - that of copper sulfate

A

EQUAL/EXCEEDS

238
Q

HEMOGLOBIN DETERMINATION

If the drop rises, its SG - that of copper sulfate

A

LESS

239
Q

HEMOGLOBIN DETERMINATION

SPECIFIC GRAVITY OF COPPER SULFATE

A

1.053

240
Q

HEMOGLOBIN DETERMINATION

ACCEPTABLE DROP OF BLOOD WILL SINK WITHIN 15SEC IF HB CONC IS -

A

> 12.5 G/DL

241
Q

HEMOGLOBIN DETERMINATION

-METHOD
VAN SLYKE OXYGEN CAPACITY METHOD

A

GASOMETRIC METHOD

242
Q

HEMOGLOBIN DETERMINATION

-METHOD
KENNEDY’S , WONG’S

A

CHEMICAL METHOD for IRON CONTENT

243
Q

HEMOGLOBIN DETERMINATION

1 G OF HGB= -OXYGEN

A

1.34 ML

244
Q

HEMOGLOBIN DETERMINATION

1 G OF HGB= -IRON

A

3.47 MG

245
Q
  • IS ABOUT 3X OF HGB VALUE
A

HEMATOCRIT

246
Q

APPLIES ONLY ON NORMOCYTIC NORMOCHROMIC RBCS

DISCREPANCY=
FIRST INDICATION OF ERROR

A

RULE OF THREE

247
Q

RULE OF THREE

HCT

A

HGB X 3

248
Q

RULE OF THREE

HGB

A

RBC X 3

249
Q

HCT/PCV

REPORTED AS -

A

36% OR
0.36 L/L

250
Q

HEMATOCRIT DETERMINATION

ADULT MALES:

A

CU: 40-54%
SI: 0.40-0.54 L/L

251
Q

HEMATOCRIT DETERMINATION

ADULT FEMALES:

A

CU: 35-49%
SI: 0.35-0.49 L/L

252
Q

HEMATOCRIT DETERMINATION

NEWBORN

A

CU: 53-65%
SI: 0.53 TO 0.65 L/L

253
Q

HEMATOCRIT DETERMINATION

-HCT

ANEMIA
HEMODILUTION

A

DECREASED

254
Q

HEMATOCRIT DETERMINATION

  • HCT

POLYCYTHEMIA
HEMOCONCETRATION

A

INCREASED

255
Q

HEMATOCRIT DETERMINATION
DIRECT METHOD (MACROMETHOD)

METHOD: WINTROBE AND LANDSBERG
ANTICOAGULANT: -

A

DOUBLE OXALATE

256
Q

HEMATOCRIT DETERMINATION
DIRECT METHOD (MACROMETHOD)

METHOD: VAN ALLEN, HADEN, SANFORD
ANTICOAGULANT: -

A

SODIUM OXALATE

257
Q

HEMATOCRIT DETERMINATION
DIRECT METHOD (MACROMETHOD)

METHOD: BRAY
ANTICOAGULANT: -

A

HEPARIN

258
Q

HEMATOCRIT DETERMINATION
DIRECT METHOD (MACROMETHOD)

SPEED OF CENTRIFUGATION

A

2000-2300G FOR 30MINS

259
Q

HEMATOCRIT DETERMINATION
DIRECT METHOD (MACROMETHOD)

WINTROBE TUBE: RED USED FOR -
- AT TOP

A

ESR, 0 AT TOP

260
Q

HEMATOCRIT DETERMINATION
DIRECT METHOD (MACROMETHOD)

WINTROBE TUBE: WHITE USED FOR -
- AT TOP

A

HEMATOCRIT, 10 AT TOP

261
Q

HEMATOCRIT DETERMINATION
DIRECT METHOD (MACROMETHOD)

WINTROBE TUBE
LENGTH: -

A

11.5 CM/115MM

262
Q

HEMATOCRIT DETERMINATION
DIRECT METHOD (MACROMETHOD)

WINTROBE TUBE
BORE: -

A

3.0 MM

263
Q

HEMATOCRIT DETERMINATION
DIRECT METHOD : -

SPX WHOLE BLOOD USING K2 EDTA OR CAPILLARY BLOOD

A

ADAM

264
Q

HEMATOCRIT DETERMINATION
DIRECT METHOD: ADAM

  • THOUGHT TO CAUSE 2-3% DECREASE IN HCT
A

K3 EDTA

265
Q

HEMATOCRIT DETERMINATION
CAPILLARY TUBE/MICROHEMATOCRIT TUBE

LENGTH: -

A

7-7.5CM
70-75MM

266
Q

HEMATOCRIT DETERMINATION
CAPILLARY TUBE/MICROHEMATOCRIT TUBE

BORE

A

1-1.2MM IN DIAMETER

267
Q

HEMATOCRIT DETERMINATION
CAPILLARY TUBE/MICROHEMATOCRIT TUBE

CAN HOLF UP TO - OF BLOOD

A

0.05ML

268
Q

HEMATOCRIT DETERMINATION
CAPILLARY TUBE/MICROHEMATOCRIT TUBE

PLUG: -

A

4-6MM

269
Q

HEMATOCRIT DETERMINATION
CAPILLARY TUBE/MICROHEMATOCRIT TUBE

TYPE
WITH - BAND= WITH ANTICOAGULANT HEPARIN

A

RED

270
Q

HEMATOCRIT DETERMINATION
CAPILLARY TUBE/MICROHEMATOCRIT TUBE

TYPE
WITH - BAND= WITHOUT ANTICOAGULANT

A

BLUE

271
Q

HEMATOCRIT DETERMINATION
CAPILLARY TUBE/MICROHEMATOCRIT TUBE

CENTRIFUGE FOR - AT RCF 10,000-15,000XG

A

5 MINUTES

272
Q

HEMATOCRIT DETERMINATION
CAPILLARY TUBE/MICROHEMATOCRIT TUBE

CENTRIFUGE FOR 5 MINS AT RCF -

A

10,000-15,000 XG

273
Q

HEMATOCRIT DETERMINATION
CAPILLARY TUBE/MICROHEMATOCRIT TUBE

AFTER CENTRIFUGATION HCT MUST BE READ WITHIN -

A

10MINS

274
Q

LAYERS OF SPUN HEMATOCRIT

Normall, layer is barely visible
In the presence of lipidemia, layer
several mm thick

A

TOP LAYER
FATTY LAYER

275
Q

LAYERS OF SPUN HEMATOCRIT

Normally pale yellow and fairly clear
Excessive hemolysis results in cherry red
color
Jaundice produces a deep yellow color

A

SECOND LAYER
PLASMA

276
Q

LAYERS OF SPUN HEMATOCRIT

Normally less than 1 mm thick

Thick layer when white cell count exceeds
10,000 cu/mm
Packed platelets are found in upper part
layer

A

THIRD LAYER
BUFFY COAT

277
Q

LAYERS OF SPUN HEMATOCRIT

Volume (packed) is read as hematocrit

A

FOURTH LAYER
PACKED RED CELL

278
Q

ERRORS IN MICROHEMATOCRIT RESULTS

FALSE - HCT

HEMOCONCENTRATION
DEHYDRATION
INSUFFICIENT CENTRIFUGATION TIME
BUFFY COAT IS READ
ALLOWING TUBE TO STAND LONGER

A

FALSE INCREASE

279
Q

HEMATOCRIT DETERMINATION

TRAPPED PLASMA MAY CAUSE HCT TO FALSELY INCREASE AS MUCH AS -%

A

1-3%

280
Q

THESE ARE CALCULATED TO DETERMINE AVERAGE VOLUME AND HGB CONTENT AND CONCENTRATION OF RBC IN SAMPLE

A

RBC INDICES

281
Q

SERVES AS QUALITY CONTROL CHECK

USED FOR INITIAL CLASSIFICATION OF ANEMIA

A

RBC INDICES

282
Q

RBC/ WINTROBE INDICES

32-36 G/DL

A

NORMOCHROMIC

283
Q

RBC/ WINTROBE INDICES

<32

A

HYPOCHROMIC

284
Q

RBC/ WINTROBE INDICES

> 36

A

HYPERCHROMIC

285
Q

RBC/ WINTROBE INDICES

AN MCHC BETWEEN 36-38 G/DL SHOULD BE CHECKED FOR -

A

SPHEROCYTES

286
Q

RBC/ WINTROBE INDICES

MCHC >38 CAUSED BY -

A

COLD AGGLUTININ

287
Q

RBC/ WINTROBE INDICES

MCHC >38= COLD AGGLUTININ
INCUBATE SPX AT

A

37C, 15MINS

288
Q

RBC/ WINTROBE INDICES

MCV: <80
MCHC: <32

A

MICROCYTIC, HYPOCHROMIC

289
Q

RBC/ WINTROBE INDICES

MCV: 80-100
MCHC: 32-36

A

NORMOCYTIC, NORMOCHROMIC

290
Q

RBC/ WINTROBE INDICES

MCV: >100
MCHC: 32-36

A

MACROCYTIC, NORMOCHROMIC