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Bacte LAB > MORPHOLOGY And SMEAR PREPARATION > Flashcards

MORPHOLOGY And SMEAR PREPARATION Flashcards

1
Q

Bacterial Morphological Types

A

A. Cocci
B. Bacilli
C. Spiral

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2
Q

Bacterial Structures

A

A. Spore
B. Capsules
C. Granule
D. Flagella

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3
Q

Laboratory Preparations

A

A. Wet Mount
B. Hanging Drop
C. Fixed Stained Smear

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4
Q

causative agent for pneumonia

A

Streptococcus pneumoniae

COCCI

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5
Q

-> causative agent for skin infections

A

Staphylococcus aureus

COCCI

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6
Q

Types of COCCI

A

Monococcus
Diplococcus
Staphylococcus
Tetracoccus
Sarcina
Streptococcus

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7
Q

COCCI
•_______ in shape
In GREEK. it means “______”

A

SPHERICAL or ROUND

BERRY

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8
Q

BACILLI
• shape..

In LATIN, it means “_____’

A

Straight, Cigarette-shaped, Sausage, Elongated

LITTLE STICKS

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9
Q

Types of BACILLI

A

Bacillus
Coccobacillus
Palisades
Diplobacillus
Spore-former
Streptobacillus

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10
Q

-› also termed as “Chinese letter”

A

Palisades

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11
Q

causative agent for botulism or food poisoning

A

Bacillus

Clostridium botulinum

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12
Q

-› found in Yakult

A

Coccobacillus

Lactobacillus casei

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13
Q

-› causative agent for diphtheria

A

Palisades

Corynebacterium diphtheria

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14
Q

SPIRAL
• shape

A

CURVED RODS, HELICOIDAL, CORK-SCREW

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15
Q

Curved shapes of bacteria

A

Vibrious
Spirilla
Spirochetes

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16
Q

-> causative agent for cholera

A

Comma

Vibrio cholerae

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17
Q

-› causative agent for syphilis

A

Spirochete

Treponema pallidum

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18
Q

• SPHERICAL or ROUND in shape
In GREEK. it means “BERRY”

A

COCCI

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19
Q

• Straight, Cigarette-shaped, Sausage, Elongated In LATIN, it means “LITTLE STICKS’

A

BACILLI

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20
Q

• CURVED RODS, HELICOIDAL, CORK-SCREW

A

SPIRAL

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21
Q

• Actual body parts of the bacteria
• Makes bacteria unique
• Narrows down the bacteria need to be identified
• Cannot be demonstrated using a routine staining method

A

BACTERIAL STRUCTURES

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22
Q

SPORE
• Example:

A

Bacillus anthracis

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23
Q
  • resting stage of bacteria;

highly resistant to extreme conditions

A

Endospore

24
Q

• The structure that forms inside bacteria

Common or routine stains cannot penetrate it

A

Endospore

25
Q

Endospore

The ability to resist stain may be attributed to its chemical composition:

A

Calcium
dipicolinic acid
peptidoglycan complex

26
Q

• substance making spore highly resistant to physical and chemical antimicrobial agents

A

Calcium, dipicolinic acid, peptidoglycan complex

27
Q

> not synthesized by vegetative cell only synthesized during spore formation

A

• dipicolinic acid

28
Q

• Only one is formed from a single bacterium
• Not a process for multiplication but formed as a defense mechanism

A

Spore

29
Q

Spore found at the tip of bacterium -*

A

terminal

30
Q

Spore found near the tip of bacterium -›

A

subterminal

31
Q

• Spore at the middle of bacterium -›

A

central

32
Q

CAPSULES
• Example:

A

Streptococcus pneumoniae

33
Q

• GLYCOCALYX or SUGAR COAT

A

Capsules

34
Q

-> A general term used for substances surrounding the cell
- a gelatinous substance that the cell secretes externally

A

GLYCOCALYX or SUGAR COAT

35
Q
  • The formation is dependent on where the bacteria grew
  • help in determining treatment for a disease
A

GLYCOCALYX or SUGAR COAT

36
Q

Glycocalyx/ sugar coat
-> made up of_______ or _____ or _____

A

polysaccharide or polypeptide or both

37
Q

2 types of glycocalyx

A

Capsule
Slime layer

38
Q

› firmly attached to the cell wall of bacteria;

responsible for protecting pathogenic bacteria from phagocytosis

A

capsule

39
Q

-› used for adhesion

A

slime layer

40
Q

The background will be____; the capsule appears as a____ zone

A

stained

clear

41
Q

GRANULE
Example:

A

Corynebacterium diphtheria

42
Q

-> store carbohydrates (stain brown with iodine)
- “iogen” granules

A

Granule

43
Q

• Referred to as inclusion bodies

• Demonstrated by staining and other chemical means

A

Granules

44
Q

Granules are formed when

A

abundant nutrients from the environment are being deposited on reserves in the cell -> granules/inclusion podies

45
Q

“Little whips”
• Thin processes usually longer than the bacteria they originate from

A

FLAGELLA

46
Q

› fibers come together to make up
flagella;
It undergo expansions and contractions; contractile (same with the concept of actin and myosin)

A

Flagellim

47
Q

Types of flagella arrangement

A

Monotrichous
Lophotrichous
Amphitrichous
Peritrichous

48
Q

LABORATORY PREPARATION AND EXAMINATION FOR MOTILITY AND MORPHOLOGY

A. WET MOUNT
• Bacteria examined must be___

A

alive

49
Q

Wet mount

Process:
- Bacterial sample (from broth or agar) is placed on a drop on____ or ____ on the slide
-› Cover with a coverslip. Examine under microscope

A

NSS or deionized water

50
Q

HANGING DROP PREPARATION
• Bacteria examined must be___
• Make use of a glass slide with a____ on it (can be made from paraffin wax) or use commercially prepared slides

A

alive

ring

51
Q

Hanging drop preparation

• Process:
- Coverslip is added with a drop of____
-> The glass slide is allowed to stick with the coverslip
- Drop that was placed on the coverslip is “hanging” if glass slide is added and placed under the microscope

A

bacteria

52
Q

FIXED STAINED SMEAR
• There is a need to____ the bacteria
• The____ procedure will render bacteria immobile

A

kill

staining

53
Q

• Bacteria examined must be alive
• Make use of a glass slide with a ring on it (can be made from paraffin wax) or use commercially prepared slides

A

HANGING DROP PREPARATION

54
Q

• There is a need to kill the bacteria
• The staining procedure will render bacteria immobile

A

FIXED STAINED SMEAR

55
Q

-> is a type of preparation that bacteriologists use to start the determination of bacterial identity

A

• Bacterial Smear

56
Q

• Bacterial Smear

  • bacteria is heated to be fixed on the slide; heat fixing must be done___
    ->_____ coagulate which will make visualization on the slide better
    -> cells will become distorted to some extent
A

rapidly

cytoplasmic proteins

Bacte LAB (15 decks)

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