EQUIPMENT USED IN BACTERIOLOGY

Question 1

_____ used for the measurement of mass in the sub- milligram range.

Answer 1

analytical balance

Question 2

are made with a measuring pan enclosed in a transparent covering that prevents smalls particles or air currents from getting collected on the pan.

Answer 2

Analytical balance

Question 3

Measures bacterial culture media components

Always calibrated before every use

Answer 3

Analytical balance

Question 4

An______ is a pressurized chamber
used for the process of sterilization and disinfection by combining three factors: time, pressure and steam

Answer 4

autoclave

Question 5

Autoclave is a pressurized chamber
used for the process of sterilization and disinfection by combining three factors:

Answer 5

time
pressure
steam

Question 6

Autoclave

• Use_____ as their sterilization agent.

Answer 6

steam

Question 7

The basic principle of an_____ is that all the items within it come in direct contact with the steam for a particular period irrespective of the nature of the material- whether it is liquid, plastic ware, or glassware.

Answer 7

autoclave

Question 8

• To be effective, the autoclave must reach and maintain a temperature of__ for at least ____by using saturated steam under at least____ of pressure.

Answer 8

121° C

15 minutes

15 psi

Question 9

a standard tool used in laboratories, named after Robert Bunsen.

Answer 9

Bunsen burners

Question 10

Bunsen burners are named after

Answer 10

Robert Bunsen

Question 11

• It is a gas-fueled single open flame.

Answer 11

Bunsend burner

Question 12

• It is commonly used for processes like sterilization, combustion, and heating.

In medical or microbiology laboratories, it is commonly used for micro-loop sterilization.

Answer 12

Bunsen Burner

Question 13

• For the bacteria incinerator, sterilization is obtained by means of infrared rays.

A complete sterilization is reached between 5/8 seconds at 850°C.

Answer 13

Bunsen Burner

Question 14

• For the_____, sterilization is obtained by means of infrared rays.

A complete sterilization is reached between_____ sec at _____ C

Answer 14

bacteria incinerator

5/8 seconds at 850°C.

Question 15

A laboratory______ is motor-based and allows the rotation of a liquid sample resulting in the separation of the components of the mixture.

Answer 15

centrifuge

Question 16

Separates liquid components by spinning

Answer 16

Centrifuge

Question 17

is used to estimate the density of a liquid culture by counting the number of CFU (colony forming units) on an agar or culture plates.

Answer 17

colony counter

Question 18

Estimates bacterial count

Answer 18

Colony counter

Question 19

is a stand-alone appliance used in microbiology laboratories as a tabletop heating system.

Answer 19

hot plate

Question 20

melting agar for culture media preparation

Answer 20

Hot plate

Question 21

• It is usually used for mixing various liquid components in a mixture in a chemical or microbiology laboratory.

Answer 21

MAGNETIC STIRRER

Question 22

• This device is used in place of other stirrers as it is noise-free and because the size of the stir bar is so tiny, there is less chance of contamination.

Answer 22

Magnetic stirrer

Question 23

Rotating magnetic field to rotate liquid

Answer 23

Magnetic stirrer

Question 24

A______ is an electrical device that is used for sterilization of medical equipment or samples using dry heat.

Answer 24

hot air oven

Question 25

can be used to sterilize materials like glassware, metal equipment, powders, etc.

• It allows for the destruction of microorganisms as well as bacterial spores.

Answer 25

• Hot air oven

Question 26

is a device that is used in the laboratories for the growth and maintenance of microorganisms and cultures.

Answer 26

incubator

Question 27

• provides an optimal temperature, pressure, moisture, among other things required for the
growth of microorganisms.

Answer 27

Incubator

Question 28

• An enclosed, ventilated laboratory workspace for safely › working with materials contaminated with pathogens requiring a defined biosafety level.

Answer 28

BIOLOGICAL SAFETY CABINET

Question 29

Biosafety cabinet

• This device creates a sterile environment with the flow of sterile air through a _______ and _______that sterilizes the workstation.

Answer 29

High-Efficiency Particulate Air (HEPA) filter

shortwave ultraviolet germicidal lamp

Question 30

Ised for initial isolation

Answer 30

Class I Biosafety cabinet

Question 31

E. coli and SAU

Answer 31

Biosafety CLASS I

Question 32

Routinely used biosafety cabinet

Answer 32

Class II

Question 33

Biosafety cabinet for anthrax, smallpox, ebola

Answer 33

Class III

Question 34

• Is a device that encloses a working area in such a way as to protect workers from aerosol exposure to infectious disease agents.

Answer 34

Biological Safety Cabinet (BSC)

Question 35

• Air that contains the infectious material is sterilized, either by______/______ or ________

• The air-handling system of a microbiology laboratory should, move.air.from. _____ to _____risk areas,/never.the reverse.

• Most hospital clinical microbiology laboratory technologists use _____cabinets.

Answer 35

heat/UVlight or by passage through a HEPAfilter - High Efficiency Parliculate Air

lower to higher

class Il

Question 36

• allow room (unsterilized) air to pass into the cabinet and around the area and material within, sterilizing.(HEPA).only. the air.to.be.exhausted.
• are open-fronted with negative pressure (ventilated cabinets).
• use for BSL2 and 3 agents.

Answer 36

Class I Cabinets

Question 37

Class II BSC

types: Class Ila, (_______) and Glass:lIB«(________)

Used for (2) agents

Answer 37

fixed,opening

variable sash,opening

BSL 2 and 3

Question 38

• provide the most protection to the worker - highest level of safety.
• air coming into and, going out of the, cabinet is sterilized (HEPA) and the infectious material within is handled.with.rubbergloves that are attached and sealed to the Cabinet.

Answer 38

Class Ill Cabinets

Question 39

can be used for the preservation of different things used in the laboratories for a very long period of time.

Answer 39

A deep freeze

Question 40

are used in laboratories to store and preserve medical equipment, food items, blood samples, medicines, and injections, etc. for a more extended period of time.

Answer 40

Deep freezers

Question 41

are devices that allow the observer to an exceedingly close view of minute particles.

Answer 41

Microscopes

Question 42

Visualize minute organisms

Observe gran staining morphology

Answer 42

Microscopes