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Bacte LAB > COLONIAL MORPHOLOGY > Flashcards

COLONIAL MORPHOLOGY Flashcards

1
Q

IMPORTANCE OF COLONIAL MORPHOLOGY

A

• Provide a presumptive identification to the physician

• Enhances the quality of patient care through rapid reporting of results and by increasing the cost-effectiveness of laboratory testing.

• Play a significant role in quality control, especially of automated procedures and other commercially available identification procedures.

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2
Q

Lactose-fermenting, gram-negative rods producing_____ colonies on MacConkey (MAC) agar.

A

pink

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3
Q

Nonlactose-fermenting, gram-negative rods producing____ colonies on MAC agar.

A

colorless

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4
Q

Lactose-fermenting Escherichia/Citrobacter-like organisms growing on MacConkey (MAC) agar.

Notice the____ appearance of the colony and the____ precipitate of bile salts extending beyond the periphery of the colonies.

A

dry

pink

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5
Q

Klebsiella/Enterobacter-like lactose fermenters growing on MacConkey (MAC) agar.

Notice the ___, ___, _____appearance.

A

pink, umbonate, mucoid

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6
Q

GROSS COLONY CHARACTERISTICS

A

Hemolytic pattern
Form/ shape
Margin
Elevation
Density/ Opacity
Size
Consistency
Color
Pigment

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7
Q

ALPHA HEMOLYSIS
•______ hemolysis
Characterized by _____around the colonies
• Example:_____

A

GREENISH ZONES

Incomplete

Streptococcus pneumoniae

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8
Q

BETA HEMOLYSIS
•_____ lysis
Characterized by ______around the colonies
Example:

A

Complete

CLEAR ZONES

Streptococcus pyogenes

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9
Q

group A B-hemolytic streptococci (Streptococcus pyogenes), produce a __________of B-hemolysis,

• whereas others, such as group B B-hemolytic streptococci (Streptococcus agalactiae) and Listeria monocytogenes (a gram- positive rod) produce a_____, diffuse zone of B-hemolysis close to the colony.

A

wide, deep, clear zone

narrow

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10
Q

• Found in Clostridium perfringens
• Characterized by BETA HEMOLYTIC_____ zone of hemolysis due to production of theta toxin and in
ALPHA HEMOLYTIC_____ zone of hemolysis due to the presence of alpha toxin a.k.a lecithinase

A

DOUBLE ZONE OF HEMOLYSIS

inner

outer

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11
Q

Shape of bacillus

A

MEDUSA HEAD COLONIES;
LION FACE

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12
Q

SWARMING OF COLONIES
______- swarms on nonselective agar, such as blood or chocolate agar plate.

A

Proteus mirabilis

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13
Q

Diphtheroid

A

Rough

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14
Q

STARS OR COLONIES WITH FEET OR PEDICLES

A

Yeast

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15
Q

Pseudomonas aeruginosa

A

serrated

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16
Q

Actinomyces israelli

A

MOLAR TOOTH COLONIES

17
Q

Form/ shape/ margin can be described as

A

smooth, filamentous, rough or rhizoid or irregular

18
Q

Serrated

A

Pseudomonas aeruginosa

19
Q

– MOLAR TOOTH COLONIES

A

Actinomyces israelli

20
Q

SIZE

SIZE
Colonies can be described as

A

LARGE, MEDIUM, SMALL

21
Q

SIZE

Streptococcus pyogenes have
COLONIES

Staphylococcus aureus have COLONIES

A

PINPOINT

PINHEAD

22
Q

CONSISTENCY/TEXTURE
• Determined by…

A

touching the colony with a sterile loop

23
Q

Consistency

• Canne described as:
• = Nocardia spp.
• = Staphylococcus aureus
• = some Neisseria, Klebsiella
• = Diphtheriods

A

Brittle, crumbly or wrinkled

Creamy

Sticky

Dry or Waxy

24
Q

Color

Enterococcus are usually ______
• Diphtheroid are_____

A

Gray

buff colored

25
Q

= blue green (pyocyanin – pyoverdin)

A

Pseudomonas aeruginosa

26
Q

• = red (prodigiosin)

A

Serratia marcescens

27
Q

= blue

A

Kluyvera

28
Q

= violet (violacein)

A

• Chromobacterium violaceum

29
Q

= brown - black

A

Provotella malaninogenica

30
Q

DOUBLE ZONE OF HEMOLYSIS
• Found in…
• Characterized by BETA HEMOLYTIC____ zone of hemolysis (due to the production of theta toxin) and an ALPHA HEMOLYTIC___ zone of hemolysis (due to the presenc

A

Clostridium

inner

outer

31
Q

ELEVATION
• Determined by tilting the plate and looking at the side of the colony
• Described as

A

flat, circular, raised, convex, umbilicate/crateriform, umbonate

32
Q

Elevation

• Streptococcus pneumoniae are usually______
• Viridans are_____
• Beta hemolytic streptococci are generally____

A

umbilicate

umbonate

flat

33
Q

DENSITY/OPTICAL PROPERTY

• Colonies are_____ if they allow all the light to pass through

• Colonies are_____ if they allow some of the light to pass through; Streptococcus agalactiae

Colonies are______ if they do not allow the light to pass through; Staphylococcus aureus

A

TRANSPARENT

TRANSLUCENT

OPAQUE

Bacte LAB (15 decks)

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