More Scientists and DNA

Question 0

What was Linus Pauling’s contribution?

Answer 0

Discovered the secondary alpha helix structure of proteins

Question 1

What did Linus Pauling work with?

Answer 1

Proteins

Question 2

What scientist did we learn about that focused on X-ray crystallography?

Answer 2

Franklin

Question 3

Who did research with Franklin?

Answer 3

Wilkins

Question 4

Who did work with crick?

Answer 4

Watson

Question 5

_______ broke into the _______ lab and stole x-ray crystallization of DNA

Answer 5

Watson

Wilkins

Question 6

Who published the DNA structure?

Answer 6

Watson and crick

Question 7

What was an “issue” with Watson and crick’s research?

Answer 7

They were able to determine the structure of DNA by stealing information form other people and the public domain

Then provided a logical explanation of DNA transmission from the meshing together of the data

Question 8

Who received the Nobel peace prize for the structure of DNA?

Answer 8

Watson, Crick, Wilkins

Question 9

What was chargaff’s contribution?

Answer 9

number of A = number of T

And

Number of C = number of G

Question 10

Ina simple description, how did Franklin do X-ray crystallography of DNA?

Answer 10

“Spooling” DNA onto a glass rod

Semi-dry piece of DNA formed needle shape

Put on x-ray machine

See diffraction pattern

By hand, calculate the distance of a helical turn, the distance of major and minor grooves, the width

Question 11

What is the distance between bases

Answer 11

0.34nm = 3.4Å

Question 12

How many bases are in a helical turn?

Answer 12

10

Question 13

What is the distance of a helical turn?

Answer 13

3.4nm = 34Å

Question 14

What is the width of a DNA strand?

Answer 14

2nm = 20Å

Question 15

What is the major groove?

Answer 15

The big space

Question 16

What is the minor groove?

Answer 16

The smaller space

Question 17

What is at the 3’ end of DNA helix?

Answer 17

Free -OH group

Question 18

What is the difference between a dinucleotide and a base pair?

Answer 18

Dinucleotides are on the same strand

Base pairs are on opposite strands

Question 19

DNA is anti-parallel. What does that mean?

Answer 19

Arrangement is 5’ -> 3’ on one strand and the complementary strand runs 3’ -> 5’

Question 20

Individual strands of DNA exhibit _____

Answer 20

Individuality

Question 21

How many hydrogen bonds are in AT base pairs?

Answer 21

Two

Question 22

How many hydrogen bonds are in CG base pairs?

Answer 22

Three