More Scientists and DNA Flashcards
What was Linus Pauling’s contribution?
Discovered the secondary alpha helix structure of proteins
What did Linus Pauling work with?
Proteins
What scientist did we learn about that focused on X-ray crystallography?
Franklin
Who did research with Franklin?
Wilkins
Who did work with crick?
Watson
_______ broke into the _______ lab and stole x-ray crystallization of DNA
Watson
Wilkins
Who published the DNA structure?
Watson and crick
What was an “issue” with Watson and crick’s research?
They were able to determine the structure of DNA by stealing information form other people and the public domain
Then provided a logical explanation of DNA transmission from the meshing together of the data
Who received the Nobel peace prize for the structure of DNA?
Watson, Crick, Wilkins
What was chargaff’s contribution?
number of A = number of T
And
Number of C = number of G
Ina simple description, how did Franklin do X-ray crystallography of DNA?
“Spooling” DNA onto a glass rod
Semi-dry piece of DNA formed needle shape
Put on x-ray machine
See diffraction pattern
By hand, calculate the distance of a helical turn, the distance of major and minor grooves, the width
What is the distance between bases
0.34nm = 3.4Å
How many bases are in a helical turn?
10
What is the distance of a helical turn?
3.4nm = 34Å
What is the width of a DNA strand?
2nm = 20Å
What is the major groove?
The big space
What is the minor groove?
The smaller space
What is at the 3’ end of DNA helix?
Free -OH group
What is the difference between a dinucleotide and a base pair?
Dinucleotides are on the same strand
Base pairs are on opposite strands
DNA is anti-parallel. What does that mean?
Arrangement is 5’ -> 3’ on one strand and the complementary strand runs 3’ -> 5’
Individual strands of DNA exhibit _____
Individuality
How many hydrogen bonds are in AT base pairs?
Two
How many hydrogen bonds are in CG base pairs?
Three
