C0t And C-Value Flashcards
What are the units of C0?
Moles of nucleotides per liter
What does the term ‘C0T’ combine?
DNA concentration (C0) and Time in renaturation studies
What are the units of T?
Seconds
What is C0T?
The product of concentration multiplied by time
How is C0T expressed? What does it represent?
.
The C0T at which a DNA sample is half annealed
Define hybridization
When a DNA strand anneals with a complementary RNA strand to form a hybrid duplex
What are C0T curves used for?
As an estimate of DNA complexity
What is the range of DNA size?
A few kilobases to hundreds of megabases
What is a convenient way to estimate the size of small DNA?
Transmission Electron Microscopy (TEM)
Besides size, what other information does TEM provide?
Shows if the DNA is linear, circular, or supercoiled
What is the most common way to estimate DNA size?
Electrophoresis
In general terms, define a paradox
.
What is c-value?
DNA content per haploid cell
True or false: the more DNA an organism has in its cells, the more complex the organism and the more genes it has
False - there is only a rough correlation, and often the correlation falls apart
Do frogs, Lilly’s or humans have more DNA?
Lilly > frogs > humans
