More bacteriology?

Question 1

Listeria

Answer 1

GP rod, motile, non-sprore forming, food-borne transmission, psychotroph, causes diarrhea, CAMP positive, VP positive, catalase positive

Question 2

Listeria with neonates and newborns

Answer 2

High mortality rate, can be acquired in utero, can cause meningitis

Question 3

How to diagnose Listeria

Answer 3

culture of CF, blood, or amniotic fluid

Question 4

Lecithinase test

Answer 4

Uses egg yolk agar, positive will have opaque white diffuse zones, negative will have no white opaque zone

Question 5

Bacteria causing positive lecithinase test

Answer 5

C. prefringens, Listeria monocytogenes, Pseudo aeriginosa, and staph aureus

Question 6

Enterobacteraceae

Answer 6

Glucose fermenting, oxidase negative, catalase positive,

Question 7

E. Coli

Answer 7

common cause of UTIs, form biofilms, can cause neonatal meningitis, beta-hemolytic, GNB, lactose fermenting, indole positive, A/A with gas

Question 8

Identifying listeria

Answer 8

soft beta hemolysis, positive CAMP, GPCB, Catalase positive, umbrella motility, VP positive

Question 9

IMViC test

Answer 9

Indole, Methyl red, VP, citrate

Question 10

Indole

Answer 10

Positive: pink ring at top of tube
Negative: no color change

Question 11

Microdase

Answer 11

Used to differentiate staph from micrococcus, oxidase reagent confirms presence of type c cytochrome, staph has no type c cytochrome

Question 12

OK VIP

Answer 12

O = oxytoca (kleb)
K = koseri (citrobacter)
V = vulgaris (proteus)
IP = indole positive

Question 13

Citrate utilization test

Answer 13

Determines if organism can ferment citrate
Positive = blue
Negative = green

Question 14

Carbohydrate utilization test

Answer 14

reveal presence of acid and/or gas produced

Question 15

TSI

Answer 15

Red slant/red butt = no fermentation
red slant/yellow butt = only glucose fermentation
yellow slant/yellow butt = lactose and/or sucrose fermentation
Dark color = H2S produced