GNC, GNDC, and GNCB Flashcards
Neisseria gonorrhoeae
Only grows on chocolate
GNDC
Oxidase positive
Doesn’t ferment maltose
Neisseria Meningitidis
Only grows on chocolate
GNDC
Oxidase positive
Ferments maltose
Moraxella
Grows on blood and chocolate
GNC/GNCB
Oxidase and Catalase positive
Nitrate reduced
DNase positive
Acinetobacter
White colonies on blood
Pink on MAC
GNCB
Oxidase negative
Oxidize glucose and lactose
Which is associated with ICU infection?
Acinetobacter
Species of Acinetobacter we see
A. baumannii and A. lwoffii
Neisseria growth requirements
Need cysteine and iron, enriched complex media and CO2
N. meningitidis virulence factors
LPS and Capsule
N. gonorrhoeae virulence factors
Pili, OPA proteins, OMPs
Virulence factors not seen in N. gonorrhoeae
Capsule and hemolysin
N. gonorrhoeae in adults
urinary discharge, STD
N. gonorrhoeae in babies
Gonococcal ophthalmia neonatorum
NAATs
Sensitive but expensive, PCR or SDA
PCR advantages
Specific, simple, fast, inexpensive, sensitive
PCR limitations
Contamination risk
Disease associated with N. gonorrhoeae
Septic arthritis
N. Meningitidis treatment
vaccine for all serotypes
Bleeding into limbs is indicative of
disseminated meningitis
Strep Bovis
AKA strep gallolyticus or group D strep
What bacteria is a hockey puck?
moraxella
DNase
DNase test agar with methyl green
Francisella
GNCB, poorly staining
Extremely infective
Just 10 organisms can cause infection
Can humans transmit Francisella to other humans?
no
Where does Francisella survive?
Water, soil, moist hay, straw, decaying carcasses
reservoirs for Francisella
Mostly rabbits, also aquatic rodents, rats, squirrels, lemmings and mice
Vectors of Francisella
Ticks, mosquitos and biting flies
Which bacteria is a bioterrorism agent?
Tularemia
Category A bioterrorism agents
Anthrax, Plague, Tularemia, Smallpox
Tularemia vaccine
Partial protection
Legionella
GN rods
Not transmitted or spread from person to person
Requires special media (BCYE)
Poor gram stain
Culture looks like ground glass
Oxidase and Catalase positive
Strains of Legionella
L. pneumonophila and L. micdadiae
L. pneumonophila
90% of cases, majority of serotypes 1-6
L. micdadiae
Most common after L. pneumonophila
What antibiotics are in a MTM plate
Vancomycin, Nystatin, and Colistin