Molecular Genetics Flashcards
For smaller fragments( hundreds of oligonucleotids)
Polyacylamide
For separation of larger fragments( thousands of oligonucleotides)
Agarose
Enzymes that cut DNA at highly specific sequences within the molecule
Restriction endonucleases
Cleaves DNA at random sites that can be more than 1,000bp
A. Type I
B. Type II
C. Type III
A.
Cleaves DNA about 25bp from the recognition sequence
A. Type I
B. Type II
C. Type III
C.
Requires atp
Cleaves the DNA within the recognition sequence itself; requires no ATP
A. Type I
B. Type II
C. Type III
B.
DNA being sequenced acts as a template
Sanger method
DNA to be sequenced is cleaved at a specific bases by subjecting it to 4 different treatments in separate aliquots
Maxam and gilbert
Piperadine treatment
A. A+G
B. G only
C. C+T
D. C only
B.
Hydrazine treatment
A. A+G
B. G only
C. C+T
D. C only
C.
Acid; piperadine treatment
A. A+G
B. G only
C. C+T
D. C only
A.
Hydrazine in 1.5M NaCl
A. A+G
B. G only
C. C+T
D. C only
D.
Cloning vector: accepts DNA pieces about 6-10kb long
A. Phages
B. Cosmids
C. Plasmids
D. Bacterial artificial chromosome
C.
Can accept DNA fragments 10-20 kb long
A. Phages
B. Cosmids
C. Plasmids
D. Bacterial artificial chromosome
A.
Can carry DNA inserts 35-50kb long
A. Phages
B. Cosmids
C. Plasmids
D. Bacterial artificial chromosome
B.
