Molecular Genetic Techniques Flashcards
1
Q
What technique is used to determine the presence or abscence of certain DNA?
A
PCR
2
Q
What methods are used to determine the size of the PCR analysis product?
A
Gel elctrophoresis by:
- oligonucleotide ligation assay
- direct sequencing
3
Q
What is allele specific mutation detection?
A
it distinguishes between 2 allels that may only differ by a single nucleotide.
4
Q
What technique is used to detect fragile X syndrome?
A
Southern blotting
5
Q
Why do we use DNA sequencing?
A
to detect unknown mutations.
- tells you exact position of mutation
- type of mutation
- uses PCR products
6
Q
What DNA is sequenced by clonal sequencing?
A
PCR products
