Molecular Biology in Medicine

Question 1

Restriction Fragment Length Polymorphism

Answer 1

• often used in tandem with Southern blot
• is a difference in homologous DNA sequences that is detected by the presence of fragments of different lengths after digesting with specific restriction enzymes

Question 2

What macromolecule is Southern blot associated with?

Answer 2

DNA

Question 3

What two tests run on agarose?

Answer 3

Southern Blotting and northern blotting

Question 4

what 3 tests run on polyacrylamide?

Answer 4

RPA, Western blotting and Gel shift

Question 5

Northern Blot is associated with which macromolecule?

Answer 5

RNA

Question 6

How does ribonuclease protection assay measure RNA levels?

Answer 6

hybridize target RNA with labeled probe

probe is protected when you digest non-hybridized RNA with RNAse

Question 7

what macromolecule does Western blot detect?

Answer 7

protein

Question 8

What is ELISA used for?

Answer 8

used to detect proteins, pathogens and can be quantitative or qualitative

Question 9

What is Chromatin Immunoprecipitation (ChIP) used for?

Answer 9

evaluating proteins bound to DNA

ex transcription factors bound to gene promoters

Question 10

You are trying to evaluate transcription factors bound to gene promoters, what is the best molecular biology tool to use?

Answer 10

-Chromatin Immunoprecipitation

Question 11

a researcher is trying to get a set amount of DNA for analysis. He has two samples. Sample A takes 6 cycles of PCR to get the required amount while sample B take 3 cycle. Describe the difference in starting amounts

Answer 11

Sample B had 6x’s as much DNA compared to sample A

Question 12

what do microarrays measure?

Answer 12

-measure gene expression by converting RNA to cDNA which is then quantified

Question 13

what technique could be used to detect a point mutation?

Answer 13

Sanger sequencing

Question 14

electrophoresis would be associated with which methodology?
A. Northern Blot
B. Dot Blot
C. ELISA
D. Microarray
E. Klenow reaction

Answer 14

A. Northern blot

Question 15

Which pair of techniques is the most appropriate choice to determine the relative levels of 3' end processing RNA of a RNA with multiple polyadenylation sites. The differences in RNA processing efficiency at these sites would result in RNA of different lengths.
A. Western Blot and EISA
B. Southern blot and Western Blot
C. Southern blot and Northern blot
D. RPA and Northern Blot
E. Dot blot and southern blot

Answer 15

D. RPA and Northern Blot

Question 16

Assuming you want ti identify a point mutation in DNA, which of the following would be the most appropriate technique to use?
A. Northern blot
B. Microarray
C. Western blot
D. Dot blot
E. Sanger sequencing

Answer 16

E. Sanger Sequencing

Question 17

In order to detect the presence of a specific macromolecule in a blot, you would need to use a:
A. primer
B. cDNA library
C. Restriction enzyme
D. Probe
E. Expression vector

Answer 17

D. probe

Question 18

PCR typically requires which specific combination?
A. array chip, amino acids, polymerase, primer
B. DNA template, polymerase, nucleotides, primer
C. Restriction enzymes, probes, nucleotides, polymerase, gel
D. gel, primers, probes, DNA template, restriction enzyme
E. DNA template, restriction enzyme, probes, primers, nucleotides

Answer 18

B. DNA template, polymerase, nucleotides, primer