molecular biology<3 Flashcards

1
Q

what are eukaryotes Mrna structural feactures

A

semi loop - involved in regulating efficiency of translation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

define ribosomes

A

large riboneucleoproteins that synthesis polypeptides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

overview of translation

A

Mina acts as template for amino acid chain
3 bases on RNA= one amino acid
Mrna reads on ribosome
Trina brings amino acids to ribosome and join to previous

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

one amino acid is coded from by

A

triplet of bases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

how many stop codons

A

3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what is the wobble hypothesis

A

first two bp have most specify, 3rd is less stringent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

three stages of translation are

A

initiation
elongation
termination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

initiation and elongation require what

A

energy GTP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

translation what is the start codon?

A

AUG (methionie)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

termination what are the stop codons

A

UAA
UGA
UAG

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

translation takes place where

A

ribosome in cytoplasm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

what is transfer RNA

A

small pieces of DNA with a 3D structure (clover leaf)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

one end of transfer DNA has —— —– attached and other has ———–

A

amino acid

anticodon

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

how many ribosomes can work on same polypeptide?

A

several

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

what does several ribosomes working on same stretch of DNA do?

A

enables several proteins to be made at once

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

amino acid residues attach to each other with

A

peptide bond

17
Q

what do release factors do?

A

recognise stop codon abd modify ribosome to activate ribosymes which sever polypeptide from final TRNA

18
Q

what happens if DNA sequence changes

A

alternative MRNA made and therefore, alternative amino acid sequence

19
Q

name types of mutations

A

point

frameshift

20
Q

point mutation is when

A

one or a few base pairs are effected

substitutions, insetrions, deletions

21
Q

frameshift mutation is when

A

neucleotide triplets after mutation displaced

insertions/deletions

22
Q

what is substitutions

A

1 base pair replaced

23
Q

what is nonsence codon

A

codon changed to termination codon
polypeptide too short
inactive protein produced

24
Q

what is a silent mutation

A

base changed but amino acid is the same