Molecular Biochemistry Flashcards
snRNP function in? Name some antibodies?
Splicesome
i.e. Anti-smith, anti-U1 RNP
Anti-smith antibodies is highly specific for?
SLE
Splicesomes function to splice?
pre-mRNA
Three Stop Codons?
UAG, UGA, UAA
DNA polymerase III vs polymerase I
Polymerase III- Elongates leading and lagging strand
Polymerase I-Degrades RNA primer
Fluoroquinolones inhibit? What do these class of enzymes do?
Topoisomerase II and IV
Add or Remove supercoils
Telomerase activity is increased in?
Cancer cells
Frameshift vs nonsense vs missense vs silent. Rank from worst to best
Frameshift, non sense, missense, silent
Worst to Best
UGA has a mutation for UAG, what is this mutation called?
Silent=> both produce termination sequences
Sickle cell disease (substitution of glutamic acid with valine) is a example of?
missense
Nonsense mutations result in early? Why?
Stop Codon
Because the C terminal would be completely removed
Duchenne muscular dystrophy is an example of what kind of mutation?
Frameshift mutation
Frameshift mutations produce?
Truncated, nonfunctional protein
For a lac operon, a repressor protein exists when there is?
No Lactose
How does low glucose increase CAP activity?
low glucose=>increased cAMP=>CAP=>transcription