Laboratory Techniques Flashcards
1
Q
How to know blotting procedures?
A
“SNOWS DROPO DAA-D”
2
Q
With a Direct ELIZA, you are using patients blood to look for?
A
Antigens because we add antibody
“Antigens Bind Directly”
3
Q
With a Indirect ELIZA, you are using someone else
s blood to look for?
A
Antibodies
4
Q
PCR is useful as _______ tool? Examples? In what disease is it particularly useful?
A
diagnostic tool
Neonatal HIV, Herpes Encephalitits
Useful for phenylalanine in CFTR region
5
Q
RT-PCR what does it stand for and what is it useful for in detecting?
A
Reverse Transcriptase PCR
RNA viruses like HIV, enterovirus and Norwalk virus
6
Q
Southern Blotting will detect? Northern Blotting? Western Blotting? Southwestern Blotting?
A
Southern: DNA
Northern: RNA
Western: Proteins
Southwestern: Transcription Factors
