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MBIO 360 > Module 9 > Flashcards

Module 9 Flashcards

1
Q

What types of commercial I’d systems are there?

A

Minikit ID- API 20

Enterotube

Minitek

Microscan

Crystal ID

Vitek

MALDI-TOF

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2
Q

What are the positive results for API 20?

A

ONPG- yellow

ADH- red

Lys and orn decarboxylase- red

Citrate- blue

H2S- black

Urease- pink

Deaminase- red/brown

Indole- red

VP- red

Gelatin- diffuse black

Carbs- yellow

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3
Q

What are the positive results for enterotube?

A

Carbs- yellow

Gas- raised wax over glucose

Decarboxylases- purple

Indole- red

VP- red

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4
Q

What is the advantage of using minitek?

A

Flexibility of choosing different substrates

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5
Q

What can microscans be used for?

A

Species ID or susceptibility testing

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6
Q

Why is for mic acid used with MALDI-TOF?

A

Breaks down cell walls so proteins can be ionized

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7
Q

What are the advantages of MALDI-TOF?

A

Time

Cost

No purity plates

Easy training

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8
Q

What systems are there for interpreting test results?

A

Checkerboard matrix/ ID charts

Flow diagrams

Numerical coding systems- binary code specific to organism generated based on results

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MBIO 360 (14 decks)

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