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MBIO 360 > Module 5 > Flashcards

Module 5 Flashcards

1
Q

What are clue cells?

A

Squamous epithelial cells coated with gram neg/variable coccoid bacilli

Suggest vaginosis

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2
Q

What are the growth requirements for Gardnerella vaginalis?

A

Facultative anaerobe

Enhanced with CO2 and moisture

35°C

Fastidious- requires enrichment

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3
Q

What is Columbia agar base?

A

Contains starch and most growth factors

Human blood can be added (Gardnerella vaginalis gives beta hemolysis)

SPS is inhibitory

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4
Q

What nonselective media is there for Gardnerella vaginalis?

A

Chocolate agar- isovitale X

Vaginalis (V) agar- Columbia base with peptone and 5% human blood

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5
Q

What selective media is there for Gardnerella vaginalis?

A

Human blood bilayer tween (HBT) agar

Columbia base with protease peptone

Colistin

Nalidixic acid

Amphotericin B

Tween 80- enhances growth

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6
Q

What are the cultural characteristics of Gardnerella vaginalis?

A

24- tiny, pinpoint

48- 2mm, beta hemolysis, on choc tiny, grey, no surrounding zone

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7
Q

What is the presumptive ID of Gardnerella vaginalis?

A

Beta hemolysis on human BAP

Small gram neg/variable coccobacilli

Catalase pos

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8
Q

What is the definitive ID for Gardnerella vaginalis?

A

Biochem testing

Starch hydrolysis pos

Hippurate hydrolysis pos

Rafinose neg

Inhibited by S. viridans

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9
Q

What antimicrobials are used on Gardnerella vaginalis?

A

Vaginosis- metronidazole

Other sites- penicillin and ampicillin

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10
Q

What is the pathogenicity of Gardnerella vaginalis?

A

Small numbers are normal vaginal flora

Increases during vaginosis (decrease in lactobacilli, increase in pH)

Vaginal discharge- many squamous epithelial cells, few PMNs

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11
Q

What other organisms are present in bacterial vaginosis?

A

Mobiluncus- small curved gram neg rods

Bacteroides- rounded, pleomorphic gram neg rods with vacuoles

Mycoplasma hominis

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12
Q

What are the findings for bacterial vaginosis?

A

Vaginal pH > 4.7

Thin, homogenous, milky discharge (not purulent, few PMNs)

Fishy smell with KOH added

Clue cells and pos Nugent score

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13
Q

How is bacterial vaginosis diagnosed?

A

Direct smear

Lack of lactobacilli

Clue cells

Mobiluncus

Gardnerella

Bacteroides

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14
Q

What does a Nugent score indicate?

A

Whether or not a direct smear is indicative of vaginosis

High- yes

Low- no

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15
Q

What are the cellular characteristics of Gardnerella vaginalis?

A

Gram neg but variable (pos earlier)

Small pleomorphic rods

No capsules, flagella or spores

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MBIO 360 (14 decks)

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