Module 12 Flashcards
How does Proteus grow on media?
BAP- grey moist, swarming
MAC- NLF, may spread
XLD, HE, SS- good growth
Not inhibited in selective enrichment media
What is the ID of Proteus?
Glucose pos with gas
Lactose neg (NLF)
Sucrose neg for mirabilis and pos for vulgaris
H2S pos
K/A, gas, H2S for mirabilis
A/A, gas, H2S for vulgaris
Deaminase pos
Urease pos
Indole neg for mirabilis and pos for vulgaris
Ornithine pos for mirabilis and neg for vulgaris
Motile
What is the rapid ID for Proteus?
Swarming on BAP
Spot indole pos for vulgaris, neg for mirabilis
What plates are used to inhibit Proteus and prevent the swarming that may overwhelm other bacteria?
CNA/PEA
What is the clinical significance of Proteus?
NF
Nosocomial infections
Mirabilis is susceptible to ampicillin (vulgaris is resistant)
What is the treatment for Proteus?
Ciprofloxacin, third gen cephalosporins, meropenem, urinary drugs, piperacillin/tazonactam
What are the most commonly isolated Providencia species?
Rettgeri and stuartii
How does Providencia grow on media?
BAP- grey moist
MAC- NLF
XLD, HE, SS- NLF, no black centres
What is the ID results for Providencia?
Glucose pos with gas
Lactose neg (NLF)
Sucrose pos
H2S neg
K/A, may show has
ONPG neg
Deaminase pos
Urease variable
Ornithine neg
Indole pos
What is the clinical significance of Providencia?
Insignificant in stool cultures
Nosocomial infections
What is the treatment for Providencia?
Third or fourth gen cephalosporins, aztreonam, imipenem, some fluoroquinolones, aminoglycosides and SXT
How does Morganella morganii grow on media?
BAP- grey moist
MAC- NLF
XLD, HE, SS- NLF, no black centres
What are the ID results for Morganella?
Glucose pos with little gas
K/A
Lactose neg
Sucrose neg
H2S neg
Deaminase pos
Urease pos
ONPG neg
Indole pos
Ornithine pos
How is Morganella differentiated from Shigella?
Pos deaminase and urease
What is the clinical significance of Morganella?
NF
Nosocomial infections
How is Morganella treated?
Piperacillin, ticarcillin, third and fourth gen cephalosporins, carbapenems, aztreonam, fluoroquinolones, aminoglycosides and chloramphenicol
What are the three species of Yersinia that are human pathogens?
Pestis
Psuedotuberculosis
Enterocolotica
What is the optimum temp for Yersinia?
25-30°C
How does Yersinia grow on media?
BAP- tiny grey moist overnight, 2-3mm after 36-48 hrs
MAC- tiny NLF
XLD, HE, SS- tiny NLF
What media is used to isolate Yersinia?
CIN/YERS
What are the ingredients of CIN/YERS?
Peptone and yeast
Mannitol and neutral red
Crystal violet
Cefsulodin
Irgasan
Novobiocin
How do Yersinia colonies appear on CIN/YERS?
Dark red centre
Bullseye
What is the clinical significance of Yersinia enterocolitica?
Not NF
Gastroenteritis
Septicaemia
What is the treatment for Yersinia?
Aminoglycosides, SXT, 3rd gen cephalosporins, tetracyclines, fluoroquinolones, ciprofloxacin, levofloxacin, nalidixic acid
What species of Proteus are most commonly isolated?
Mirabilis and vulgaris
