Module 7 Flashcards

1
Q

What information has to be documented when inputting data?

A

Initiation of test request

Patient info

Collection/draw list and schedules

Preparation of labels

Verification of specimen collection

Accession records/log sheets

Creation of work lists

Test results

Storage of test results

Dissemination of test results

Billing

Work unit tabulation

Supply inventory/ordering

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2
Q

How are requests initiated?

A

Outpatients- paper req, staff enters data

Hospitals- req is entered into the system and will appear in the lab data files

STAT and ASAP tests are printed automatically

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3
Q

What is necessary when entering data regarding collection lists and schedules?

A

Prioritize, decide when to collect what.

Draw list indicates collection requests.

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4
Q

When should specimens be collected?

A

Outpatients- collect ASAP

Hospital- early morning, collect a specific test on a number of patients all at once

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5
Q

How is collection verified?

A

Date, time and initials or collection code is recorded on the tube and req.

Collected code can be entered to patient’s data.

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6
Q

How are accessions recorded?

A

Log sheets record collections.

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7
Q

Who is responsible for work lists?

A

Each department

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8
Q

How are results recorded?

A

On department-specific reqs.

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9
Q

How are results stored?

A

Copy of the req and results are retained by the lab.

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10
Q

How are results delivered?

A

By hand

Couriers or porters

Telephoned reports are followed up with a written report

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11
Q

What is work unit tabulation?

A

Each procedure is designated a certain number of work units depending on the complexity and time required.

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12
Q

How is specimen identification verified? What has to be checked?

A

Must ensure the specimen is suitable.

Check for correct:
Patient info
Type of specimen
Volume of specimen
Collection date and time
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13
Q

What makes a specimen unacceptable?

A

Inadequate ID

NSQ sample

Incorrect collection- may be able to use an alternate test

Hemolyzed blood samples- usable unless grossly hemolyzed

Incorrect transportation- usually performed but not with leaking specimens

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14
Q

What is the rejection process?

A

Notify supervisor

Recollect

Notify unit/physician

Enter comment

Don’t discard sample until problem is resolved

Urine and serum samples that can’t be frozen should be refrigerated for up to a week

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15
Q

How are STAT specimens handled?

A

Have priority, must be processed immediately.

Warn tech, use special centrifuge or STAT flag.

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16
Q

How are tubes centrifuged?

A

EDTA- placed on mixer, sedimentation within 12hrs, reticulocyte counts within 72hrs, refrigerated up to 24hr

Plasma- spun immediately, PST for 10min at 1000-1300G, NaCit for 15min at 1500G

Serum- allow adequate time for coag prior, red top 30-60min wait, SST 30 min wait for 10min at 1000-1300G, clot activator 5-15min wait, 37°C to speed coag

17
Q

How are specimens received on ice centrifuged?

A

Precooled 4°C centrifuge

18
Q

What do delays in separation result in?

A

Falsely increased serum/plasma values due to more K in RBCs (diffuses out).

Accelerated by refrigeration.

Serum and plasma should be separated within 2hrs, kept at room temp for no longer than 8hrs.

19
Q

What do tubes have to be inspected for after centrifugation?

A

Red tube- separation of clot and serum, free cells or fibrin strands, hemolysis

SST- barrier appearance, RBC/fibrin strands, visible hemolysis

Plasma- clots/fibrin strands, RBCs, visible hemolysis

20
Q

How are tubes handled after centrifugation?

A

Stoppers are pulled straight off.

Separation within 2hrs.

Can use direct sampling equipment or sampler devices (through stopper).

21
Q

What is aliquoting?

A

Moving a sample to another tube.

Can’t mix serum and plasma in the same tube.

22
Q

How is aliquoting organized?

A

Cross check info

Determine number of aliquot tubes, label

Remove stopper

Cross check labels on specimen and aliquot tubes

Use a fresh Pasteur pipette to aliquot

Replace tubes in proper rack position

Stopper aliquot tubes

Double check labels and distribute

23
Q

How are micro specimens collected?

A

In capillary or microtainer tubes.

1.5x the required volume.

24
Q

How are micro specimens centrifuged?

A

Microcentrifuge

3min at 2000G

25
Q

How are chilled specimens handled?

A

Refrigerated centrifuge and chilled tubes.

26
Q

What are lipemic samples?

A

Chylomicrons are fat particles in blood, make the specimen appear cloudy.

27
Q

How are lipemic samples centrifuged?

A

Ultracentrifuge

28
Q

What are examples of light sensitive specimens?

A

Neonatal bilirubin, carotene, Vit A and B6, methotrexate.

29
Q

How are light sensitive specimens stored?

A

Amber tube or wrapped in foil or brown paper bag

30
Q

How must frozen specimens be treated and what happens if they are not?

A

Must be mixed well before aliquoting.

If unmixed, aliquoted tests give low analyte values and remaining specimen gives high values.

31
Q

How long are general documents stored for?

A

Worksheets and QC records- 2yrs

Workload management data- 5yrs

Maintenance records- life of instrument plus 2yrs

Reqs- 1 month min

32
Q

How long are patient reports and charts stored for?

A

10 yrs

Paediatric- til the age of majority plus 2yrs or 10yrs (whatever’s longest)

33
Q

How long are specimens kept for?

A

Chemistry- one day after final report

Hematology- blood specimens one day after final report, normal slides after one week, abnormal slides and bone marrow after 10yrs

Transfusion medicine- specimens for 7 days after post transfusion, transfused units/segments for 7 days after post transfusion, patient data file is kept indefinitely

Microbiology- specimens for one day after final report, CSF for one week, gram stains after reading

Autopsy records- paraffin blocks/slides for 10yrs, wet tissue for 8wks after final reports

34
Q

What is the best way to perform data entry?

A

Computerization- streamlines and simplifies procedures and reduces the number of errors.