Module 2.1- Bacterial Morphology and Cytology Flashcards
The general shape of individual bacterium is usually discernible with:
light microscope
revealed distinct anatomical features of bacteria
electron microscope (1950s)
range of bacteria diameter
0.2 - 2.0 micrometers
range of length of bacteria
2-8 micrometers
three basic shapes of bacteria
> cocci
bacilli
spiral
usually spherical-shaped bacteria but can be oval, elongated or flattened on one side
cocci (clue: berries)
cocci may remain attached in groups due to
incomplete separation of cell during division
cocci that remain in pairs after division of a bacterial cell in one plane
Diplococci
cocci in chain-like patterns following division in one plane
Streptococci
cocci in groups of four resulting from division of bacterial cell in two planes
tetrads
cocci in cube-like groups of eight produced when bacterial cell divides in three planes
Sarcinae
cocci in grapelike clusters that form when bacterial cell divides in multiple planes
Staphylococci (clue: staphyle, bunch of grapes)
cocci in grapelike clusters that form when bacterial cell divides in multiple planes
Staphylococci (clue: staphyle, bunch of grapes)
rod-shaped and characteristically long and slender
Bacilli (clue: meaning little staffs)
bacilli that are oval and look so much like cocci
coccobacilli
difference between coccobacilli and cocci
Bacilli divide only across their short axis
most common type of bacilli
single rods
bacilli that appear in pairs after division
Diplobacilli
bacilli that occur in chains
streptobacilli
bacilli that are arranged in angular patterns that look like X, Y, V, and L” configuration
Cuneiforms (clue: looks like Chinese letters)
how cuneiforms form
bending of the bacterial cell at the point of division
example of cuneiform bacteria
Corynebacterium diphtheriae
bacilli in “picket fence” or cigar packet arrangement (side by side)
Palisades
how palisades form
slipping (sliding) of the bacterial cells during division
bacteria that have one or more twists; they are never straight.
spirals
spirals that look like rods curved into a form resembling a comma.
vibrios
spirals that have helical (i.e., coiling) shape, like corkscrews and have fairly rigid bodies
spirilla
spirals that are helical and flexible
spirochetes
most bacteria belong to this where they maintain a single shape
monomorphic (mono, one; morph, form)
bacteria which can have many shapes, not just one
pleomorphic (pleo, many; morph, form),
three (3) architectural regions of a bacterial cell
> cell envelope
appendages
cytoplasmic region
structure that encloses the cytoplasm of the cells, essentially the protective unit
cell envelope
three layers of the cell envelope (stratified structure)
> cell membrane
cell wall
glycocalyx
all cells have this part of the cell envelope
cell membrane
almost all bacteria have this part of the cell envelope
cell wall
only some bacteria have this part of the cell envelope
glycocalyx
thin, delicate membrane surrounding the cytoplasm and separating it from the environment.
cell membrane
two structural components of the cell membrane
> phospholipid bilayer (40%)
> proteins (60%)
two components of the phospholipid bilayer
> polar, hydrophilic glycerol head outside
> two non-polar hydrophobic fatty acid tails inward
The arrangement of the lipids and proteins to form a membrane is called the
fluid mosaic model
component that is found in eukaryotic cell membrane but not in bacterial cell membrane
sterols
only bacteria which have sterols to protect from osmotic lysis beacuse they do not have cell wall
genus Mycoplasma
Functions of the cell membrane
- selective permeability
- site of transport system
- site for biosynthesis
- specialized enzyme system
- chemotaxis
- participates in reproduction
- site of antibiotic action
The most important function of the plasma membrane
serve as a selective barrier
the property of the cell membrane that allow certain molecules to move through the membrane while restricting others
Semipermeability
uncharged molecules with molecular weights of about 100 Daltons which pass through the bacterial cell membrane freely
water
he free movement of water through the cell membrane from a region of lower solute concentration to a region of higher solute concentration
osmosis
Medium where the concentration of solutes is the same inside and outside of the cell. There is no net gain or loss of water, so the
cell will retain its original shape
isotonic medium
Medium where the solute concentration outside of the cell is lower than in the inside of the cell, and water enters the cell.
hypotonic medium
Limits a bacterium’s accumulation of water.
non expendable cell wall
The escape of the cytoplasm from cells without rigid cell walls when it ruptures due to high pressure
plasmoptysis
medium where the solute concentration is higher outside of the cell, and water leaves the cell.
hypertonic medium
Hypertonic medium causes the cell membrane and cytoplasm to shrink away from the cell wall. This is known as:
plasmolysis
cytoplasm rupture is referred to as
plasmoptysis
cytoplasm shrinking is referred to as
plasmolysis
Membrane proteins that mediate the passage of solutes through the cell membrane
carrier proteins or permeases
Because carrier proteins mediate solute passge, transport systems are therefore:
carrier-mediated
show specificity for solute
Three types of transport systems among bacteria
> facilitated diffusion
active transport
group translocation
The least common type of transport system in bacteria (e.g., glycerol uniporter in E. coli)
Facilitated diffusion
Used for transport of most solutes like amino acids, ions, and sugars
active transport
Used for accumulation of many ions, amino acids, or sugars into the bacterium
Active transport
Transports at a rate faster that diffusion alone
active transport
Used primarily for transport of sugars
(e.g., glucose specifically enters the channel from outside. but to enter the cytoplasm, it must be sequentially converted into pyruvic acid, which is the key metabolic intermediate, then bacteria process the pyruvic acid using a variety of fermentation pathways.)
group translocation
Is solute modified during facilitated diffusion?
No
Is solute modified during active transport?
No
Is solute modified during group translocation?
Yes
Does facilitated diffusio have energy expenditure?
No
Does active transport have energy expenditure?
Yes
Does group translocation have energy expenditure?
Yes
Does Facilitated Diffusion move against concentration gradient?
No
Does Active transport move against concentration gradient?
Yes
Does Group translocation move against concentration gradient?
Yes
Function that where the cell membrane allows for production of components that make up the bacterial cell wall and appendages.
Site for biosynthesis
Funnction of the cell membrane where it contains enzymes involved in many metabolic processes such as cell wall synthesis, membrane synthesis and DNA replication.
Specialized enzyme system
Bacterial ability to move/swim in response to environmental stimuli which is facilitated by their cell membrane
chemotaxis
Component of the bacterial cell membrane which determines the quality and quantity of certain chemicals in the environment
sensing protein
Bacterial cell swimming towards useful nutrients
positive chemotaxis
Bacterial cell swimming away from harmful substances
negative chemotaxis
Other types of tactic responses in bacteria which provides the evidence for the ecological advantage (survival) in bacteria.
phototaxis (towards light source)
aerotaxis (towards oxygen)
magnetotaxis (in response to Earth’s magnetic field)
reproduction of bacteria
binary fission
cytoplasmic invaginations of the cell membrane in the form of stacks or vesicles which coordinates DNA replication and segregation with septum formation
mesosomes
another function of mesosome
significantly increase the membrane surface area without increasing the cell size, allowing the cell greater activity for respiration and active transport.
What does mesosome do during cell division?
- attaches the DNA where it is replicated
- draws the 2 DNA molecules in opposite direction while the septum is formed between 2 chromosomal components
- 2 progeny cells form after septum formation
A type of antibiotic that pokes holes in the lipid bilayer
Polymyxin
chemical agents that dissolve the lipid bilayer resulting in leaking out of the cytoplasmic contents
alcohol and some detergents
how the cell dies when cytoplasmic contents are leaked
lysis
The rigid layer surrounding the cell membrane in most bacteria.
Cell wall
The strength of cell wall is primarily due to this substance which can only be found in bacteria
peptidoglycan
Other terms for peptidoglycan
murein or mucopeptide
The peptidoglycan is composed of:
Carbohydrate (CHO) backbone & peptide chains
The carbohydrate backbone of peptidoglycan is a polymer of disaccharides consisting of alternating units of
- N-acetylmuramic acid (NAM)
2. N-acetylglucosamine (NAG)
The peptide chain of peptidoglycan is composed of ________ and hence is called __________
4 amino acids
tetrapeptide
The peptide chain of peptidoglycan is attached to the:
N-acetylmuramic acid (NAM)
A single layer of peptidoglycan is a network of adjacent sugar chains bound together through the:
peptide chains