Curved Gram Negative bacilli

Question 1

IMViC: ++–

Answer 1

E. coli
Edwardsiella
Proteus

Question 2

IMViC: -+–

Answer 2

Salmonella typhi
Shigella

Question 3

IMViC: –++

Answer 3

K. pneumoniae

Question 4

hydrogen sulfide producers

Answer 4

Proteus
Edwardsiella
Salmonella

Question 5

non motile

Answer 5

Shigella
Klebsiella
Yersina enterocolitica

Question 6

DNase (+)

Answer 6

Serratia marcesens

Question 7

Gas producers

Answer 7

Klebsiella
Enterobacter
Serratia
Hafnia

Question 8

PDA (+)

Answer 8

Proteus
Morganella
Providencia

Question 9

urease posiitve

Answer 9

Klebsiella
Proteus
Morganella
Providencia

Question 10

differentiates E. coli O157:H7 from kost E. coli

Answer 10

MUG Test (-) blue fluorescence
Sorbitol MacConkey agar (-l

Question 11

test for delayed lactose fermenters (beta galactosidase and lactose permease)

Answer 11

ONPG test

Question 12

decarboxylation product of lysine

Answer 12

cadaverine

Question 13

decarboxylation product of ornithine

Answer 13

putrescine

Question 14

decarboxylation of arginine

Answer 14

citrulline

Question 15

principle of indole

Answer 15

tryptophanase production yields indole and pyruvate fron tryptone
indole reacts with p-aminobenzaldehyde creating cherry red color

Question 16

methyl red test

Answer 16

mixed acid fermentation of glucose causes acidity of indicator

Question 17

principle of VP test

Answer 17

butylene glycol pathway of glucose acetoin production
acetoin with 40% KOH and alpha naphtol produces pink diacetyl

Question 18

simmons citrate principle

Answer 18

citrate as the only source of carbon and ammonium phosphate for nitrogen causes alkalinization of medium

Question 19

recovered from feces through cold enrichment

Answer 19

Yersinia enterocolitica

Question 20

Y. enterocolitica is subculturer into

Answer 20

Cefsulodin, Irgasan, Novobiocin

Question 21

Y. enterocolitica colonies in CIN medium

Answer 21

bulls eye appearance