Ex 1 Instrumentation in Microbiology

Question 1

Three important properties of glass in microbiology laboratories

Answer 1

> transparent
stable/non reactive
easily malleable

Question 2

thin glass tube which is used to hold small amount of material (sample or culture broth) for testing

Answer 2

plain glass tube

Question 3

test tube with screw cap cover used for holding materials, especially for transport and growing of cultures in broth

Answer 3

screw capped tube

Question 4

screw capped tube with smaller test tubes inserted upside down inside it, which is used to detect production of gas by microorganisms (fermentation test)

Answer 4

Durham fermentation tube

Question 5

glass ware with a closed vertical arm extending into a bulbous portion with a tapered opening used in observing organism doing anaerobic fermentation

Answer 5

Smith fermentation tube

Question 6

shallow glass or plastic flat bottomed dish with a lid used to culture bacteria on specially prepared solid media

Answer 6

Petri Dish

Question 7

Narrow neck and mouth that enlarge gradually to a flat bottom, usually graduated which is used to swirl content without spilling

Answer 7

Erlenmeyer Flask

Question 8

Long cylindrical tubes usually held upright by a stable base that is substantially wider than the vertical stem and has calibration markers

Answer 8

Graduated cylinder

Question 9

Measuring pipettes that have graduations extending to the tip

Answer 9

serologic pipette

Question 10

disposable plastic or nichrome wire which is used to aseptically transfer microorganisms from the broth, slant, or agar cultures to other media

Answer 10

Inoculating Loop and Needle

Question 11

Stainless steel rack that holds microscopic slide during staining

Answer 11

staining rack

Question 12

plastic, steel or wooden material that allows 5 or 20 degrees tilt for media to harden at an angle for incubating liquid cultures

Answer 12

Slant rack

Question 13

An insulated and enclosed device that provides an optimal condition of temperature, humidity, and other environmental conditions required for growth of organisms

Answer 13

incubator

Question 14

A laboratory equipment made from a container filled with heated water used to incubate samples at a constant temperature over a long period of time

Answer 14

water bath

Question 15

Incubator and water bath should be recorded every:

Answer 15

every day and before opening

Question 16

Liquids usually used for cleaning glassware

Answer 16

sodium dichromate sulphuric acid cleaning solution
nitric acid
fuming sulphuric acid
alcohol
water

Question 17

How to test cleanliness of glassware

Answer 17

1. See whether reagent grade water covers the vessel with a continuous film or if little drops are left behind
2. Observe water spots inside or outside the vessel

Question 18

Process which is applied to quantitative measuring or metering of equipment to assure its accurate operation throughout its measuring limit

Answer 18

calibration

Question 19

The steps taken to confirm and record the proper operation of equipment at a given point of time in the range in which tests are performed

Answer 19

validation