Module 1.4 - Phytochemical Tests (Anthraquinone Glycosides) Flashcards
Positive Result for Borntrager’s test
Red/rose pink coloration in the lower ammonia-cal layer
Process for Borntrager’s test
- Boil test material with 1 mL of dilute sulphuric acid in a test tube for 5 min
- Centrifuge or filter while hot, filtrate, cool, and shake with an equal volume of dichloromethane
- Separate the lower dichloromethane layer and shake with hald its volume with dilute ammonia
PRESENCE OF ANTHRAQUINONES
Positive Result for Modified Borntrager’s test
- 200 mg of powdered herb with 4 mL of alcohol KOH fr 2-3 in test tube
- Dilute with 4 mL of water and filter
- Acidify with HCl
- Cool, add few drops of H2O2, shake well with 5 mL of ether
- Allow to separate into test tube
- Shake with 2 mL of dilute solution of Ammonium hydroxide
PRESENCE OF ANTHRAQUINONES
Positive result for Bate-Smith and Metcalf Test Method
Strong red or violet color
Remarks for Bate-Smith and Metcalf Test Method
Presence of Leucoantho-cyanin
Positive result for Wilstatter
“cyanidin” Test Method
Colors ranging from orange to red, Presence of y-benzopyrone nucleus: crimson, and magenta and occasionally to green or blue
Remarks for Wilstatter “cyanidin” test
Presence of y-benzopyrone nucleus
Positive result for Gelatin Test
Jelly precipitate
Remarks for Gelatin Test
Presence of tannins
Positive result for Ferric Chloride test
Blue-black color
Brownish-green color
Remarks for Ferric Chloride test
Presence of hydrolyzable tannins
Presence of nonhydrolyzable/condensed tannins
(FERRIC CHLORIDE TEST) Phenol, resorcinol, Ortho cresol, Para cresol
Violet or blue colouration
(FERRIC CHLORIDE TEST) Catechol
Green colouration
(FERRIC CHLORIDE TEST) Hydroquinone
Violet or transient blue color
(FERRIC CHLORIDE TEST) Pyrogallol
Blue rapidly changing to red
