Module 1.3 - Phytochemical Tests (Cardenolides and Bufadienolides) Flashcards
1
Q
Positive result for Keller-Killiani test
A
Purple color at the junction
2
Q
Remarks for Keller-Killiani test
A
Presence of 2 - deoxysugar
3
Q
Positive result for Lieberman-Burchard test
A
Colors ranging from blue to Green, red, pink, purple or violet
4
Q
Remarks for Lieberman-Burchard test
A
Presence of steroid/triterpenoid skeleton
5
Q
Positive result for Salkowski’s test
A
Red color
6
Q
Remarks for Salkowski’s test
A
Presence of unsaturated sterol and/or triterpenes
7
Q
Process for Keller Killiani test
A
- Extract with chloroform, evaporate to dryness
- Add 0.4 mL of glacial acetic acid containing a trace amount of ferric chloride
- Transfer to small test tube, ass 0.5 mL of conc. Sulphuric acid by the side of the test tube
- Blue color appears in the acetic acid layer
8
Q
Process for Libermann-Burchard test
A
- 2 mg of dry extract was dissolved in Acetic anhydride
- Heated to boiling
- Cooled
- add 1 mL of conc. Sulphuric acid was added along the sides of the test tube
- Formation of a pink/red color indicates triterpenoids
9
Q
Positive result for Liebermann-Burchard’s test for STEROIDS
A
Green color
10
Q
Process for Liebermann-Burchard test for STEROIDS
A
- 2 mg of dry extract dissolved in Acetic anhydride
- Heated to boiling
- Cooled
- 1 mL of conc. Sulphuric acid added to sides of test tube
11
Q
Positive result for Salkowski’s test for STEROIDS
A
Red color
12
Q
Process for Salkowski’s test for STEROIDS
A
- 2 mg of dry extract was shaken with chloroform
- Sulphuric acid slowly added to the chloroform layer by the sides of the test tube
